- Open Access
miR-335-5p inhibits TGF-β1-induced epithelial–mesenchymal transition in non-small cell lung cancer via ROCK1
Respiratory Research volume 20, Article number: 225 (2019)
Significant evidence has shown that the miRNA pathway is an important component in the downstream signaling cascades of TGF-β1 pathway. Our previous study has indicated that miR-335-5p expression was significantly down-regulated and acted as a vital player in the metastasis of non-small cell lung cancer (NSCLC), however the underlying mechanism remained unclear.
The differential expression level of miR-335-5p and ROCK1 were determined by qRT-PCR and IHC analysis in human tissue samples with or without lymph node metastasis. Transwell assay was conducted to determine cell ability of migration and invasion. SiRNA interference, microRNA transfection and western blot analysis were utilized to clarify the underlying regulatory mechanism.
We showed that down-regulated expression of miR-335-5p and up-regulated expression of ROCK1 in NSCLC tissues were associated with lymph node metastasis. Over-expresion of miR-335-5p significantly inhibited TGF-β1-mediated NSCLC migration and invasion. Furthermore, luciferase reporter assays proved that miR-335-5p can bind to 3′-UTR of ROCK1 directly. Moreover, we confirmed that siRNA-mediated silencing of ROCK1 significantly diminished TGF-β1-mediated EMT and migratory and invasive capabilities of A549 and SPC-A1 cells.
This is the first time to report that miR-335-5p regulates ROCK1 and impairs its functions, thereby playing a key role in TGF-β1-induced EMT and cell migration and invasion in NSCLC.
Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related death worldwide [1, 2]. Although great progress has been made, the five-year survival rate of NSCLC patients are still below 15% . It is reported that more than 90% of deaths are attributed to metastasis in solid tumors, including NSCLC . Hence, in the study of the definition of the mechanism in NSCLC metastasis, a detailed understanding should be very important.
MicroRNAs (miRNAs) belong to small noncoding RNAs and the roles they play in tumor development are mediated by downstream signaling networks which can further regulate multiple cellular functions like proliferation and migration [5,6,7,8]. In particular, comprehensive expression analysis has shown that multiple miRNAs contributed to the development of lung cancer [9, 10]. Among them, miR-335-5p is mainly reported to function as a tumor suppressor of migration and invasion. In gastric cancer, miR-335-5p acts as a metastasis suppressor via targeting Bcl-w and specificity protein 1 . MiR-335-5p over-expression can inhibit MCF-7 and MDA-MB-231 cell proliferation and motility partially by directly attenuating EphA4 expression in breast cancer . Research in colorectal cancer indicated that patients with reduced miR-335-5p expression had a poorer overall survival and that miR-335-5p over-expression can not only inhibit tumor cells migration and invasion in vitro but also inhibit lung and liver metastasis in vivo, suggesting as a potential therapeutic target for colorectal cancer . The role of miR-335-5p played in bladder cancer (BC) showed that miR-335-5p could suppress BC cell proliferation and migration by up-regulating CRKL expression . However, how miR-335-5p functions in mediating tumor metastasis in NSCLC is poorly known [15, 16].
On the basis of miRNA arrays shown in our previous study, down-regulated expression of miR-335-5p is reported in NSCLC tissues when compared to adjacent tissues . In another previous study, we suggested that miR-335-5p functions as a crucial regulator in the pathogenesis of NSCLC. Over-expression of miR-335-5p in A549 and H226 lung cancer cells can inhibit cell proliferation and metastasis through down-regulating CPNE1 expression, mediated via inactivation of EGFR signaling pathway . Here, we predicted new potential target mRNAs of miR-335-5p using computational algorithms that may involved in metastasis of NSCLC. Interestingly, among all possible target mRNAs, we mainly focus on Rho associated protein kinase 1 (ROCK1). ROCK1 is a serine/threonine kinase protein and a direct downstream effector of Rho-GTPase signaling [19, 20]. So far the well-established role of ROCK1 supported a positive function in tumor growth. There is a growing body of evidence indicated that up-regulated expresion of ROCK1 is closely associated with β-catenin and c-myc, and thus involved in cell proliferation, cell apoptosis and cell cycle control [21, 22]. In addtion, ROCK1 can regulate tumor cell migration and invasion partially based on its role in regulating actin cytoskeleton [23, 24]. Growing evidence has also shown that the RhoA/ROCK axis can be activated as another noncanonica downstream signaling pathway in TGF-β1-induced epithelial-mesenchymal transition (EMT). It is demonstrated that LncRNA DANCR /miR-27a-3p axis can regulate EMT via ROCK1/LIMK1/COFILIN1 pathway in hepatocellular carcinoma progression . In human colon adenocarcinoma, ART1 could regualte EMT process by regulating ROCK1/AKT/β-catenin pathway . In NSCLC, FPPS could induce TGF-β1-induced lung cancer cell invasion and EMT via RhoA/ROCK1 pathway . Interestingly, we found that ectopic miR-335-5p could inhibit TGF-β1-induced EMT and invasion of NSCLC cells.
It is well known that the transforming growth factor β1 (TGF-β1) superfamily takes crucial part in cell proliferation, apoptosis, differentiation and angiogenesis [28, 29]. As a double-edged sword, TGF-β1 inhibits cell growth in early stages . In contrast, TGF-β1 can also induce EMT to promote tumor cell invasion in some degree . EMT is significant for morphogenesis during the process of embryonic development and for early-stage tumors in conversion into advanced invasive malignancies, along with suppressed expression of E-cadherin and induction of N-cadherin and Vimentin [31, 32]. Accumulating evidence shows that microRNAs contribute to TGF-β1 signaling-induced EMT in various cancers. Autocrine TGF-β/ZEB /miR-200 signaling network is active and can regulate epithelial-mesenchymal transition in breast cancer . In NSCLC, our previous study proved that miR-205 can moderate TGF-β1-induced EMT in A549 cell lines via repression on smad4 .
Our current study is to focus on elucidating the mechanism of metastasis regarding miR-335-5p in NSCLC. Data from our analysis indicated that miR-335-5p can suppress TGF-β1-induced EMT by binding to ROCK1 directly in NSCLC. Our study provided crucial evidence to illuminate the molecular and cellular mechanism of miR-335-5p-mediated suppression of metastasis, which suggested that miR-335-5p may be a promising therapeutic target for highly aggressive NSCLC.
Materials and methods
Sixty NSCLC tissue samples were collected from patients between 2012 and 2016 at the respiratory department of the First Affiliated Hospital of Soochow University. All participants were provided with written informed consent at the time of recruitment. All cases had clinically and pathologically confirmed diagnoses of NSCLC in accordance with the Revised International System for Staging Lung Cancer. Meanwhile none of these cases had received any other treatments before tissue sampling. All collected samples were further stored in a freezed condition at − 80 °C.
ROCK1 and miR-335-5p expression in NSCLC and correlation analysis with EMT markers and overall survival
The GEPIA database (http://gepia.cancer-pku.cn/) was used to generate survival curves between ROCK1 expression and overall survival of NSCLC patients. Meanwhile we extracted data from oncoLnc database (http://www.oncolnc.org/) to validate the association between miR-335-5p expression and overall survival. To further verify the association between miR-335-3p or ROCK1 expression and EMT status in patient samples, we applied the Linkedomics database (http://www.linkedomics.org) to retrieve data on ROCK1 or miR-335-5p expression and EMT-associated marker like N-cadherin, E-cadherin, Vimentin and Snail. Meanwhile MMPs family members like MMP2 and MMP9 were also involved.
Immunohistochemical (IHC) analysis was conducted in our previou study . Briefly, the sections were incubated with ROCK1 antibody (diluted to 1:100; Santa Cruze Technology, sc-17,994) overnight at 4 °C, and then incubated with the biotinylated secondary antibodies. The reactions were developed using the DAB Kit (BD Bioscience, San Jose, CA, USA), and the sections were counterstained with hematoxylin.
All cell lines in this study including 16HBE, A549, HCC827, H1299, H1975, SPC-A1, H226, H1650 and H460 were obtained from the Cell bank of the Chinese Academy of Sciences (Shanghai, China) and maintained in Gibco-1640 or RPMI-1640 medium (Sigma, South Logan, UT, USA) supplemented with 10% fetal bovine serum (Gibco, South America, SA, USA) and antibiotics (Invitrogen, CA, USA) at 37 °C in a humidified incubator which contains 5% CO2. In some experiments, cells were cultured in special medium containing additional 5 ng/ml TGF-β1 for 48 h.
Plasmid construction, and luciferase reporter assay
A549 and SPC-A1 cells were used for luciferase assay. In order to generate psiCHECK2-ROCK1–3′-UTR-wild type and relevant mutant plasmids, a 233 bp fragment located in the position 472–478 of ROCK1 3′- UTR which contains a potential miR-335-5p target sequence or a mutated fragment were cloned into psiCHECK2 dual luciferase vector (Promega, Madison, WI, USA). Cells were plated in 24-well plate at a optimum density of 2 × 104/well and cotransfected with wild or mutant synthetic plasmid along with miR-335-5p mimics or miR-NC using jetprime reagent (Life Technologies) at the following day. 24–48 h later, cells were lysed and Dual-Luciferase Reporter Assay kit (Promega) was carried out to examine luciferase activity. Renilla luciferase activities were setted as inner control for the comparision of firefly luciferase. Each experiment was performed for three times.
MiR-335-5p mimics, plasmid siRNA, and cell transfection
A549 and SPC-A1 cells were seeded in 6-well plates. When cells had reached 40–60% confluence, we performed transfection in accordance with the manufacturer’s instructions using jetprime reagent (Invitrogen). Cells were collected at 48–72 h after transfection for further experiments. MiR-335-5p mimics, ROCK1 siRNA (Si-ROCK1) and corresponding control were purchased from GenePharma company (Suzhou, China). The target siRNA sequences were as follows: ROCK1si-1 5′-GCAGAUGAAACAGGAAAUA-3′, ROCK1 si-2 5′-GGCAGAGGAAGAAUAUAAA-3′.
Wound healing assay
After transfection for 48 h with miR-335-5p or si-ROCK1, A549 and SPC-A1 cells were plated into a 6-well plate with a density of 2.5 × 105/well. At day 2, we gently scratched the monolayer using a 10 μl pipette tip and make sure the tip perpendicular to the bottom of the well during the process. After scratching, the wells were gently washed twice with PBS to remove the detached cells and replaced with fresh medium for an additional 12–24 h. The representative images of gap distance were acquired with microscope and quantitatively analysed and plotted with Photoshop.
Migration and invasion assay
Transwell chambers (Corning, New York, NY, USA) were used to test the cell migration and invasion ability. For migration assay, cells were suspended and plated on uncoated chambers. The only difference between these two assays was that the inserts in invasion assay were needed to be pre-coated with diluted Matrigel matrix (BD Science, Sparks, MD, USA) at 37 °C for 2 h. For both assays, tumor cells were transfected with si-NC or si-ROCK1 for 48 h. In some conditions, cells were transfected with miR-NC or miR-335-5p mimics for 48 h, and then cultured in medium containing TGF-β1. Cells were trypsinized, and then 5 × 104 cells diluted in 200 μl medium with 1% FBS were seeded into the upper champer. 800 μl RPMI-1640 medium with 10% FBS was added to the bottom of each chamber. After incubation for 24 h, non-invasive cells were removed and the remaining cells attached the inserts were fixed with methanol for 30 min, then dry for 10 min in room temperature, stained with 0.1% crystal violet overnight to make staining completely. At last we washed inserts three times with PBS. Cells were then photographed and counted. Each experiment was performed in triplicate.
RNA extraction, cDNA synthesis, and quantitative real-time PCR analysis
RNAiso Plus reagent (Takara, Osaka, Japan) was used to extract total RNA from cells and tissues in accordance with the manufacturer′s protocol. 1 μg of total RNA was reverse-transcripted using a reverse transcriptase M-MLV kit (Takara, Osaka, Japan). mRNA expression was detected by qPCR using SYBR Master Mixture (Takara, Osaka, Japan) on an ABI Step One Plus Real-Time PCR system (Applied Biosystems, Foster City, CA, USA). Primers used for qRT-PCR were supplied by Guangzhou Ribo BioCompany (Guangzhou, China). The detailed sequences involved in this study were showed in Additional file 1 Table S1. β-actin and U6 were used as internal control. The 2-ΔΔCt method was applied to figure out the relative expression. Each experiment was repeated in triplicate.
Western blot assay
PBS was needed to be pre-cooled before experiment. After washed with PBS and 1 x RIPA lysis buffer was added to cells (Cell Signaling Technology, Danvers, MA, USA) with 1:100 phosphatase inhibitor and 1:100 protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA). 10% SDS-PAGE was used to separate protein and then transferred to nitrocellulose membranes (Millipore, Billerica, MA, USA). At room temperature, membranes were blocked with 5% BSA or 5% milk for 1 h which was then dissolved in TBST buffer containing 0.1% Tween-20. Later we incubated with corresponding primary antibodies overnight at 4 °C and the appropriate secondary antibodies. After washing four times with TBST, protein detection was performed by chemiluminescence (Pierce, Rockford, IL, USA). Antibodies used in this research included anti-MMP2 (D8N9Y), anti-N-cadherin, anti-Vimentin (RV202, BD Biosciences, USA), anti-Snail (C15D3, Cell Signaling Technology, Danvers, MA, USA), and anti-mouse or rabbit secondary antibodies (Cell Signaling Technology).
All data are presented as mean ± standard deviation (SD). P < 0.05 was set as statistically significant analyzed by Student’s t-test. All statistical analysis were calculated using GraphPad Prism 6.0 (GraphPad, San Diego, CA, USA) and SPSS 17.0 software (SPSS, Chicago, IL, USA).
MiR-335-5p over-expression inhibits the migratory ability of NSCLC cells
To evaluate the function of miR-335-5p in NSCLC, we first over-expressed miR-335-5p and then assessed the possible effects of miR-335-5p on cell migration. Results from transwell assay showed that over-expression of miR-335-5p can inhibit the migratory ability in both A549 (Fig. 1a) and SPC-A1 cells (Fig. 1b). Wound healing assay was further carried out to verify the function of miR-335-5p in regulating cell migration of A549 and SPC-A1 cells. As demonstrated in Fig. 1c, when compared with transfection with miR-NC, cells transfected with the miR-335-5p mimics lower the speed migrating towards the scratch. Consistent with these observations, qRT-PCR analysis and western blot analysis revealed decreased expression levels of Snail, N-cadherin,Vimentin and MMP2 in cells over-expressed miR-335-5p (Fig. 1d and e). In addition, no significant changes were detected regarding MMP9 and other associated transcriptional factors like Slug, ZEB1 and ZEB2 (Additional file 2 Figure S1).
MiR-335-5p inhibits TGF-β1-induced EMT, cell migration and invasion of NSCLC cells
In next step, the transwell assays were performed to clarify how miR-335-5p functions to induce cell invasion mediated by TGF-β1. We found that the TGF-β1-induced increase of migration and invasion abilities were inhibited after transfection with miR-335-5p in A549 or SPC-A1 cells (Fig. 2a and b). Furthermore, we performed western blot analysis to deeply clarify the role of miR-335-5p in TGF-β1-induced EMT in NSCLC cells. As shown in Fig. 2c, miR-335-5p over-expression significantly inhibited the TGF-β1-induced up-regulation of Snail, N-cadherin, Vimentin and MMP2 in both A549 and SPC-A1 cells. Our results suggested that miR-335-5p inhibited TGF-β1-induced migration and invasion in NSCLC cells.
MiR-335-5p targets ROCK1 via 3′-UTR-binding in NSCLC cells
To explore the mechanism by which miR-335-5p regulates NSCLC cell progression, we searched for potential regulatory targets of miR-335-5p on the basis of bioinformatics methods. Using publicly available databases (Target Scan Human: http://www.targetsca n.org/), we identified that miR-335-5p can bind to the complementary sequence of 3′-UTR of ROCK1 (Fig. 3a). To validate this hypothesis, ROCK1 wildtype and mutated reporter plasmids were generated and A549 and SPC-A1 cells were used to perform dual-luciferase reporter assays. The data manifested that the luciferase activity was largely inhibited with miR-335-5p over-expresssion when cells transfected with wildtype construct but did not with the mutant construct (Fig. 3b and c). In addition, qRT-PCR and western blot analysis exhibited that ROCK1 expression in A549 and SPC-A1 cells was detected to be reduced after transfection with miR-335-5p mimics (Fig. 3d and e). Collectively, our data certified that miR-335-5p can target 3′-UTR of ROCK1 mRNA directly in NSCLC cells.
Reduced miR-335-5p expression and increased ROCK1 expression are associated with lymph node metastasis in NSCLC tissues
The expression level of miR-335-5p and ROCK1 in 60 paired NSCLC tissues were further classified to subgroups according to presence or absence of lymph node metastasis (Additional file 3 Table S2). Reduced miR-335-5p expression was associated with lymph node metastasis in NSCLC tissues when compared to NSCLC tissues without metastasis (Fig. 4a). Meanwhile, based on data extracted form GEPIA and oncoLnc database, we further explored that patients with higher miR-335-5p expression was associated with more favorable overall survival (Fig. 4b). In particular, we found that miR-335-5p expression was negatively related with MMP2 and Vimentin expression in 447 patient samples from linkedomics database (Fig. 4c). In addition to miR-335-5p, up-regulated ROCK1 expression was found in NSCLC tissues with lymph node metastasis, displaying poorer overall survival (Fig. 4d and e). And linkedomics database indicatd that ROCK1 expression was positively related with MMP2 and Vimentin expression in 515 patient samples (Fig. 4f). Moreover, we performed IHC analysis to compare ROCK1 expression in 10 patients samples with or without lymph node metastasis seperately. The data demonstrated that ROCK1 was mainly located in cell cytoplasm and membrane. In lymph node metastasis group, ROCK1 expression was found to be higher in 6 NSCLC tissues out of 10 NSCLC tissues. However, in 10 non-lymph node meatstasis tissues, only 2 tissues showed high ROCK1 expression. And the representative images were shown in Fig. 4g.
Knockdown of ROCK1 inhibits TGF-β1-induced EMT, cell migration and invasion of NSCLC cells
Multiple studies indicated that ROCK1 functions as an oncogene, and that ROCK1 over-expression promotes invasion and metastasis in lung cancer cells . However, the role of ROCK1 has not been fully explored in TGF-β1-induced EMT, especially in lung cancer. To address this, we first inhibited ROCK1 using two specific small interfering RNAs (siRNAs) and found that ROCK1 expression was significantly reduced in A549 and SPC-A1 cells (Fig. 5a and b). In wound healing assays, cells transfected with si-ROCK1 migrated towards the scratch more slowly (Fig. 5c and d). Transwell assay showed similar results (Fig. 5e and f). Moreover, western blot revealed decreased protein levels of Snail, N-cadherin, Vimentin and MMP2 in cells with ROCK1 knockdown (Fig. 5g).
Next, the role ROCK1 played in promoting metastasis of NSCLC cells induced by TGF-β1 was evaluated. In the presence of TGF-β1, knockdown of ROCK1 can obviously diminished EMT and the migration and invasion of A549 and SPC-A1 cells (Fig. 6a and b). Consistent with these observations, western blot analysis confirmed that knockdown of ROCK1 noticeably inhibited the TGF-β1-induced up-regulated expression of Snail, N-cadherin, Vimentin and MMP2 in A549 and SPC-A1 cells (Fig. 6c).
A majority of lung cancer patients are already at an advanced stage of tumor progression when diagnosed at the first time . One of the most studied mechanisms of tumor metastasis is TGF-β1-mediated EMT, which is associated with the conversion of early-stage tumors into invasive malignancies . TGF-β1-mediated signal transduction functions to promote EMT in many cancers like lung cancer . TGF-β1-induced EMT in malignant cells is characterized as suppressed expression of E-cadherin along with increased expression of N-cadherin and Vimentin .
It is well known that TGF-β1 activates multiple intracellular signaling molecules to mediate cellular functions indepedent of Smads. RhoA/ROCK family function as downstream effectors in the TGF-β1-induced non-Smad signaling pathways [35, 36]. ROCK1 and ROCK2 are two isoforms in ROCK family and display similarities in their kinase activity domains . Human ROCK1 locates in chromosome 18 (18q11.1) . ROCK1 is proved to function as a positive regulator in promoting invasion and metastasis in many types of solid tumors, including bladder , hepatocellular carcinoma , breast cancer  and lung cancers [42, 43].
In this study, we first elucidated how miR-335-5p regulated NSCLC metastasis. EMT can be induced by TGF-β1 signal in NSCLC , and a study reported that miRNA-335-5p can bind to genes involved in non-canonical TGF-β1 signalling pathway to suppress cell invasion . We verified the impact of miR-335-5p on EMT induced by TGF-β1 in NSCLC cells. Our data demonstrated that NSCLC cells over-expressing miR-335-5p showed decreased migration and invasion abilities. Meanwhile siRNA-mediated silencing of ROCK1 distinctly diminished EMT induced by TGF-β1 and the migration and invasion in A549 and SPC-A1 cells. Further, we revealed that down-regulated expression of miR-335-5p and up-regulated expression of ROCK1 in NSCLC tissues were closely relevant to lymph node metastasis. Taken together, our data suggested that miR-335-5p over-expression can negatively regulate the expression of ROCK1 and TGF-β1-induced EMT and cell migration and invasion in NSCLC cells.
In conclusion, our study is the first to report that miR-335-5p regulates ROCK1 and impairs its functions, thereby playing a key role in TGF-β1-induced EMT and cell migration and invasion in NSCLC (Fig. 6d). Our findings illuminate the inner binding interaction between ROCK1 and miR-335-5p in NSCLC metastasis, and underscore the significance of miR-335-5p as a novel target for therapeutic intervention in advanced NSCLC.
Availability of data and materials
The original source data and material will be available upon resonable request. And the Kaplan-Meier survival curves were obtained from GEPIA database (http://gepia.cancer-pku.cn/) and oncoLnc database (http://www.oncolnc.org/). The association analysis is available in the Linkedomics database (http://www.linkedomics.org).
Non-small cell lung cancer
Rho associated protein kinase 1
Real-time polymerase chain reaction
Transforming growth factor-β1
Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, et al. Cancer statistics in China, 2015. CA Cancer J Clin. 2016;66(2):115–32.
Siegel RL, Miller KD, Jemal A. Cancer statistics, 2017. CA Cancer J Clin. 2017;67(1):7–30.
Wang T, Nelson RA, Bogardus A, Grannis FW Jr. Five-year lung cancer survival: which advanced stage nonsmall cell lung cancer patients attain long-term survival? Cancer. 2010;116(6):1518–25.
Gupta GP, Massague J. Cancer metastasis: building a framework. Cell. 2006;127(4):679–95.
Guo H, Ingolia NT, Weissman JS, Bartel DP. Mammalian microRNAs predominantly act to decrease target mRNA levels. Nature. 2010;466(7308):835–40.
Markou A, Tsaroucha EG, Kaklamanis L, Fotinou M, Georgoulias V, Lianidou ES. Prognostic value of mature microRNA-21 and microRNA-205 overexpression in non-small cell lung cancer by quantitative real-time RT-PCR. Clin Chem. 2008;54(10):1696–704.
Lu J, Getz G, Miska EA, Alvarez-Saavedra E, Lamb J, Peck D, et al. MicroRNA expression profiles classify human cancers. Nature. 2005;435(7043):834–8.
Lebanony D, Benjamin H, Gilad S, Ezagouri M, Dov A, Ashkenazi K, et al. Diagnostic assay based on hsa-miR-205 expression distinguishes squamous from nonsquamous non-small-cell lung carcinoma. J Clin Oncol. 2009;27(12):2030–7.
Yanaihara N, Caplen N, Bowman E, Seike M, Kumamoto K, Yi M, et al. Unique microRNA molecular profiles in lung cancer diagnosis and prognosis. Cancer Cell. 2006;9(3):189–98.
Cai J, Fang L, Huang Y, Li R, Yuan J, Yang Y, et al. miR-205 targets PTEN and PHLPP2 to augment AKT signaling and drive malignant phenotypes in non-small cell lung cancer. Cancer Res. 2013;73(17):5402–15.
Xu Y, Zhao F, Wang Z, Song Y, Luo Y, Zhang X, et al. MicroRNA-335 acts as a metastasis suppressor in gastric cancer by targeting Bcl-w and specificity protein 1. Oncogene. 2012;31(11):1398–407.
Dong Y, Liu Y, Jiang A, Li R, Yin M, Wang Y. MicroRNA-335 suppresses the proliferation, migration, and invasion of breast cancer cells by targeting EphA4. Mol Cell Biochem. 2018;439(1–2):95–104.
Sun Z, Zhang Z, Liu Z, Qiu B, Liu K, Dong G. MicroRNA-335 inhibits invasion and metastasis of colorectal cancer by targeting ZEB2. Med Oncol. 2014;31(6):982.
Liu XK, Chen D, Li X. MiR-335 suppresses cell proliferation and migration by upregulating CRKL in bladder cancer. Eur Rev Med Pharmacol Sci. 2019;23(6):2399–408.
Chen QY, Jiao DM, Yan L, Wu YQ, Hu HZ, Song J, et al. Comprehensive gene and microRNA expression profiling reveals miR-206 inhibits MET in lung cancer metastasis. Mol BioSyst. 2015;11(8):2290–302.
Luo LJ, Wang DD, Wang J, Yang F, Tang JH. Diverse roles of miR-335 in development and progression of cancers. Tumour Biol. 2016;37(12):pp15399–15410.
Zhu J, Zeng Y, Xu C, Qin H, Lei Z, Shen D, et al. Expression profile analysis of microRNAs and downregulated miR-486-5p and miR-30a-5p in non-small cell lung cancer. Oncol Rep. 2015;34(4):1779–86.
Tang H, Zhu J, Du W, Liu S, Zeng Y, Ding Z, et al. CPNE1 is a target of miR-335-5p and plays an important role in the pathogenesis of non-small cell lung cancer. J Exp Clin Cancer Res. 2018;37(1):131.
Dvorsky R, Blumenstein L, Vetter IR, Ahmadian MR. Structural insights into the interaction of ROCKI with the switch regions of RhoA. J Biol Chem. 2004;279(8):7098–104.
Jacobs M, Hayakawa K, Swenson L, Bellon S, Fleming M, Taslimi P, et al. The structure of dimeric ROCK I reveals the mechanism for ligand selectivity. J Biol Chem. 2006;281(1):260–8.
Schofield AV, Bernard O. Rho-associated coiled-coil kinase (ROCK) signaling and disease. Crit Rev Biochem Mol Biol. 2013;48(4):301–16.
Rath N, Munro J, Cutiongco MF, Jagiello A, Gadegaard N, McGarry L, et al. Rho kinase inhibition by AT13148 blocks pancreatic ductal adenocarcinoma invasion and tumor growth. Cancer Res. 2018;78(12):3321–36.
Di Blasio L, Gagliardi PA, Puliafito A, Primo L. Serine/Threonine Kinase 3-Phosphoinositide-Dependent Protein Kinase-1 (PDK1) as a Key Regulator of Cell Migration and Cancer Dissemination. Cancers (Basel). 2017;9(3). https://doi.org/10.3390/cancers9030025.
Loirand G. Rho kinases in health and disease: from basic science to translational research. Pharmacol Rev. 2015;67(4):1074–95.
Guo D, Li Y, Chen Y, Zhang D, Wang X, Lu G, et al. DANCR promotes HCC progression and regulates EMT by sponging miR-27a-3p via ROCK1/LIMK1/COFILIN1 pathway. Cell Prolif. 2019;52(4):e12628.
Song GL, Jin CC, Zhao W, Tang Y, Wang YL, Li M, et al. Regulation of the RhoA/ROCK/AKT/beta-catenin pathway by arginine-specific ADP-ribosytransferases 1 promotes migration and epithelial-mesenchymal transition in colon carcinoma. Int J Oncol. 2016;49(2):646–56.
Lin L, Li M, Xu X, Jiang G, Wu L. FPPS mediates TGF-beta1-induced non-small cell lung cancer cell invasion and the EMT process via the RhoA/Rock1 pathway. Biochem Biophys Res Commun. 2018;496(2):536–41.
Massague J. TGF-beta signal transduction. Annu Rev Biochem. 1998;67:753–91.
Kitisin K, Saha T, Blake T, Golestaneh N, Deng M, Kim C, et al. Tgf-Beta signaling in development. Sci STKE. 2007;2007(399):cm1.
David CJ, Huang YH, Chen M, Su J, Zou Y, Bardeesy N, et al. TGF-beta tumor suppression through a lethal EMT. Cell. 2016;164(5):1015–30.
Kang Y, Massague J. Epithelial-mesenchymal transitions: twist in development and metastasis. Cell. 2004;118(3):277–9.
Thiery JP. Epithelial-mesenchymal transitions in tumour progression. Nat Rev Cancer. 2002;2(6):442–54.
Gregory PA, Bracken CP, Smith E, Bert AG, Wright JA, Roslan S, et al. An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition. Mol Biol Cell. 2011;22(10):1686–98.
Zeng Y, Zhu J, Shen D, Qin H, Lei Z, Li W, et al. Repression of Smad4 by miR205 moderates TGF-beta-induced epithelial-mesenchymal transition in A549 cell lines. Int J Oncol. 2016;49(2):700–8.
Moustakas A, Heldin CH. Non-Smad TGF-beta signals. J Cell Sci. 2005;118(Pt 16:3573–84.
Zhang YE. Non-Smad pathways in TGF-beta signaling. Cell Res. 2009;19(1):128–39.
Montalvo J, Spencer C, Hackathorn A, Masterjohn K, Perkins A, Doty C, et al. ROCK1 & 2 perform overlapping and unique roles in angiogenesis and angiosarcoma tumor progression. Curr Mol Med. 2013;13(1):205–19.
Lock FE, Ryan KR, Poulter NS, Parsons M, Hotchin NA. Differential regulation of adhesion complex turnover by ROCK1 and ROCK2. PLoS One. 2012;7(2):e31423.
Kamai T, Tsujii T, Arai K, Takagi K, Asami H, Ito Y, et al. Significant association of rho/ROCK pathway with invasion and metastasis of bladder cancer. Clin Cancer Res. 2003;9(7):2632–41.
Xue F, Takahara T, Yata Y, Xia Q, Nonome K, Shinno E, et al. Blockade of rho/rho-associated coiled coil-forming kinase signaling can prevent progression of hepatocellular carcinoma in matrix metalloproteinase-dependent manner. Hepatol Res. 2008;38(8):810–7.
Lane J, Martin TA, Watkins G, Mansel RE, Jiang WG. The expression and prognostic value of ROCK I and ROCK II and their role in human breast cancer. Int J Oncol. 2008;33(3):585–93.
Chen J, Ye L, Zhang L, Jiang WG. Placenta growth factor, PLGF, influences the motility of lung cancer cells, the role of rho associated kinase, Rock1. J Cell Biochem. 2008;105(1):313–20.
Vigil D, Kim TY, Plachco A, Garton AJ, Castaldo L, Pachter JA, et al. ROCK1 and ROCK2 are required for non-small cell lung cancer anchorage-independent growth and invasion. Cancer Res. 2012;72(20):5338–47.
Lynch J, Fay J, Meehan M, Bryan K, Watters KM, Murphy DM, et al. MiRNA-335 suppresses neuroblastoma cell invasiveness by direct targeting of multiple genes from the non-canonical TGF-beta signalling pathway. Carcinogenesis. 2012;33(5):976–85.
We thank all patients who participated in this study for their cooperation. We also thank the native English speaking scientists of Elixigen Company (Huntington Beach, California) for editing our manuscript.
This work was supported by grants from the Postgraduate Research & Practice Innovation Program of Jiangsu Province (No. KYCX18_2525), the Suzhou Key Laboratory for Respiratory Medicine (No. SZS201617), the Clinical Medical Center of Suzhou (No. Szzx201502), the Jiangsu Provincial Key Medical Discipline (No. ZDXKB2016007), the Projects of Medical Science and Technology Development Plan of Yancheng City (No. YK2017003 to H-C. Tang).
Ethics approval and consent to participate
All participants were provided with written informed consent at the time of recruitment. And this study was approved by the Ethics Committee of the First Affiliated Hospital of Soochow University.
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The sequences of primers involved in our study.
The expression of MMP9 and associated transcriptional factors Slug, ZEB1 and ZEB2 after transfected with miR-335-5p mimics in A549 and SPC-A1 cells.
Clinicopathological features of NSCLC tissues from 30 patients with non-lymph node metastasis and 30 patients with lymph node metastasi.
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Du, W., Tang, H., Lei, Z. et al. miR-335-5p inhibits TGF-β1-induced epithelial–mesenchymal transition in non-small cell lung cancer via ROCK1. Respir Res 20, 225 (2019). https://doi.org/10.1186/s12931-019-1184-x
- Non-small cell lung cancer
- Transforming growth factor beta 1
- Rho-associated protein kinase 1