Fig. 1

Over-expression of miR-335-5p inhibits NSCLC cell motility and migration and invasion. a-b Transwell assay of the cell migratory and invasive activity in A549 and SPC-A1 cell lines transfected with miR-335-5p mimics. c A wound-healing assay was performed to observe the effect of miR-335-5p transfection in cells. d The expression level of N-cadherin, Vimentin and E-cadherin by quantitative RT-PCR analysis in A549 and SPC-A1 cell lines with miR-335-5p over-expression. e Western blot analysis of Snail, N-cadherin, Vimentin, and MMP2 after transfected with miRNA mimics (miR-335-5p or miR-NC) for 48 h. **P < 0.01; ***P < 0.001