Fig. 6

Knockdown of ROCK1 significantly inhibits TGF-β-induced EMT and cell migration and invasion of NSCLC cells. a-b. Transwell assay of the ROCK1-silenced A549 and SPC-A1 cells after treated with or without TGF-β1 (5 ng/ml) for 24 h. c. Western blot analysis of Snail, N-cadherin, Vimentin, and MMP2 expression in A549 and SPC-A1 cells after siRNA (si-ROCK1 or si-NC) transfection for 24 h and treated with exogenous TGF-β1 (5 ng/ml) for additional 24 h. d. A working model of the mechanistic interaction of miR-335-5p and ROCK1 in the control of TGF-β1-induced cell migration and invasion. ***P < 0.001