- Open Access
Complementary roles of KCa3.1 channels and β1-integrin during alveolar epithelial repair
© Girault et al. 2015
Received: 25 February 2015
Accepted: 21 August 2015
Published: 4 September 2015
Extensive alveolar epithelial injury and remodelling is a common feature of acute lung injury and acute respiratory distress syndrome (ARDS) and it has been established that epithelial regeneration, and secondary lung oedema resorption, is crucial for ARDS resolution. Much evidence indicates that K+ channels are regulating epithelial repair processes; however, involvement of the KCa3.1 channels in alveolar repair has never been investigated before.
Wound-healing assays demonstrated that the repair rates were increased in primary rat alveolar cell monolayers grown on a fibronectin matrix compared to non-coated supports, whereas an anti-β1-integrin antibody reduced it. KCa3.1 inhibition/silencing impaired the fibronectin-stimulated wound-healing rates, as well as cell migration and proliferation, but had no effect in the absence of coating. We then evaluated a putative relationship between KCa3.1 channel and the migratory machinery protein β1-integrin, which is activated by fibronectin. Co-immunoprecipitation and immunofluorescence experiments indicated a link between the two proteins and revealed their cellular co-distribution. In addition, we demonstrated that KCa3.1 channel and β1-integrin membrane expressions were increased on a fibronectin matrix. We also showed increased intracellular calcium concentrations as well as enhanced expression of TRPC4, a voltage-independent calcium channel belonging to the large TRP channel family, on a fibronectin matrix. Finally, wound-healing assays showed additive effects of KCa3.1 and TRPC4 inhibitors on alveolar epithelial repair.
Taken together, our data demonstrate for the first time complementary roles of KCa3.1 and TRPC4 channels with extracellular matrix and β1-integrin in the regulation of alveolar repair processes.
Extensive damage and remodelling of the alveolar epithelium occur in various lung pathologies, including acute lung injury (ALI) and its more severe form, acute respiratory distress syndrome (ARDS) [1–3]. Alveolar regeneration, which is crucial to restore alveolar epithelial integrity and function, is thus a critical component of ARDS resolution and patient recovery [1, 2, 4].
After damage, several cellular events are engaged in an attempt to restore alveolar integrity, including changes in cell-matrix adhesion through the action of matrix metalloproteinases and integrin receptors, cytoskeleton reorganization, cell spreading and migration, as well as cell proliferation and differentiation . These complex processes integrate multiple mechanisms and proteins, which are regulated by various components such as growth factors, growth factor receptors and downstream signalling pathways [5–7]. Integrins play an active role in epithelial repair, not only by creating a link between the ECM and cell cytoskeleton but also by interacting with proteins involved in cell migration and proliferation, including growth factor receptors, protein kinases as well as ion channels [8–11]. β1-integrin, for example, has been shown to regulate alveolar type II (ATII) cell migration on fibronectin matrix . Moreover, increased levels of fibronectin and collagen have been detected in lung tissues from patients with ARDS .
Increasing evidence also indicates a function of potassium (K+) channels in the regulation of epithelial repair processes . More precisely, silencing or inhibition of different types of K+ channels has been reported to decrease epithelial cell proliferation [15–18], motility [15, 16, 19–23] and differentiation , as well as epithelial wound repair [15, 16, 24–26]. Our data on primary rat ATII cells previously highlighted an involvement of two types of K+ channels, i.e. KvLQT1 and KATP, in the control of cell proliferation, motility and repair . A role for KCa3.1 channels in airway ion transport [27, 28], as well as repair processes of several epithelial tissues [16, 22, 29] has also been established; however, the contribution of this channel in alveolar repair has not been explored before.
The mechanisms whereby K+ channels control epithelial repair processes may be multiple, including changes in membrane potential, cell volume and shape, [Ca2+]i and various signalling pathways (for review see ). In addition, several reports indicated that different types of K+ channels (e.g. BKCa, Kv1.3, hERG, GIRK, Kir4.2) could also directly interact with migratory machinery proteins, such as β1-integrins [30–33]. However, to the best of our knowledge, a relationship between the KCa3.1 and β1-integrin in epithelial cells has never been investigated before.
Based on these data, we postulated that KCa3.1 and β1-integrin play a complementary role during alveolar epithelial repair. We thus evaluated the roles of extracellular fibronectin matrix, β1-integrin and KCa3.1 channels in alveolar repair processes, especially cell migration, proliferation and wound healing after mechanical injury. Finally, the regulation and complementary function of TRPC4 Ca2+ channels were explored.
Alveolar epithelial type II cell isolation and primary culture
Alveolar epithelial cells were isolated from rat lungs according to a procedure approved by our institutional animal care committee (CIPA) of Centre de Recherche du Centre Hospitalier de l’Université de Montréal (CRCHUM) in accordance with the Canadian Council of Animal Care (CCAC) standards. Alveolar epithelial type II (ATII) cells were isolated from adult male Sprague–Dawley rats (6–7 weeks), according to a well-established protocol [15, 34–36]. In brief, the lungs were washed to remove blood cells and alveolar macrophages before treatment with elastase (Worthington, Lakewood, NJ, USA). They were then minced, and the resulting suspension was filtered. Alveolar cells were collected and purified using a differential adherence technique , which enhances the purity of the ATII cell pool up to 86 % [35, 38]. Although most of the macrophages are bound on IgG coated plates during this differential adherence technique, some retained in the final post-IgG cell mix (along with some red blood cells), where they likely constituted a significant proportion of non-alveolar cells in the cell prep at day 0 . This freshly isolated cell suspension was seeded on Petri dishes (Corning, Fisher Scientific Ltd., Nepean, ON, Canada) or on glass slides (VWR International, Mississauga, ON, Canada) and adherent alveolar epithelial cells were cultured in minimal essential medium (MEM; Gibco, Life Technologies Inc., Burlington, ON, Canada) containing 10 % FBS (Gibco, Life Technologies Inc.), 0.08 mg/l gentamicin, septra (3 μg/ml trimethoprime + 17 μg/ml sulfamethoxazole), 0.2 % NaHCO3 (Sigma-Aldrich, Oakville, ON, Canada), 10 mM HEPES (Hyclone, Fisher Scientific Ltd.), and 2 mM L-glutamine (Gibco, Life Technologies Inc.), as previously described [15, 35]. The MEM-FBS-septra medium was replaced after 3 days by the same MEM-FBS without septra.
ATII cells, cultured for 3–4 days, were injured mechanically with a pipette tip (six wounds per Petri dish) according to a highly reproducible technique [15, 16, 39–42]. This commonly employed “wound-healing assay” allows for the study of early mechanisms engaged after injury, i.e. cell migration and proliferation . A mark on the Petri dishes enabled us to photograph the wounds at exactly the same place at various times (at time 0 after injury and after 24 h of repair). The rate of wound closure, presented in μm2/h, was calculated with ImageJ software (National Institutes of Health, Bethesda, MD, USA) from the wound area measured after repair compared with the initial wound area, for each wound.
ATII cells were seeded at low density (52,000/cm2) in 35-mm Petri dishes for 3 days in MEM-FBS-septra medium and then exposed or not to TRAM-34 (Sigma-Aldrich) for a 24-h period. Cell growth was evaluated by counting the number of ATII cells by separation with trypsin-EDTA (0.05 %, Gibco, Life Technologies Inc.) before (at day 3, T0) and after (at day 4, T24h) treatment. The absence of drug cytotoxicity was verified by trypan-blue exclusion assay. The number of ATII cells was also counted at day 3 after transfection with negative control or KCa3.1 siRNAs (see section on siRNA transfection).
Cell migration assays
ATII cell migration was first evaluated by Boyden-type chamber assays, as previously described [15, 39]. Briefly, primary ATII cells were separated with trypsin-EDTA (Gibco, Life Technologies Inc.), counted, and their viability was verified with Trypan blue assay (Sigma-Aldrich). A series of experiments was also performed on ATII cells previously transfected with negative control or KCa3.1 siRNAs (see section on siRNA transfection). The cell suspensions (75,000 cells in FBS-free MEM) were placed in the upper compartment of 8-μm pore filters (0.33 cm2, ThinCerts-TC inserts, Greiner Bio-one; MJS Biolynx, Brockville, ON, Canada) coated on the lower side with a gelatin (control condition, Sigma-Aldrich) or fibronectin matrix (Sigma-Aldrich). The lower compartment was filled with FBS-free MEM in the absence or presence of TRAM-34. After an 18-h migration period, the filters were washed with PBS, the cells were fixed with paraformaldehyde-acetone solution and then stained with hematoxylin (Sigma-Aldrich). Non-migrating cells in the upper compartment were scraped off with cotton-tipped applicators (Fisher Scientific Ltd.), whereas migrating cells on the lower face of the filters were counted in different randomly chosen fields under a light-inverted microscope at x20 magnification. Two-D cell migration rates (μm/h) of single cells were also evaluated by single-cell tracking in live-video microscopy experiments. Images were captured at 5-min intervals over a 24-h period by digital camera connected to Zeiss microscope. The migration rates and cell trajectories were analyzed by AxioVision software (Carl Zeiss, Jena, Germany).
Immunoblotting of enriched ATII membrane fractions and co-immunoprecipitation of KCa3.1 and ß1-integrin
For enriched membrane fraction assays, ATII cells were scraped into TRIS-sucrose buffer (20 mM TRIS HCl (Life Technologies Inc.), 5 mM EDTA (ACP, Montréal, QC, Canada), 200 mM sucrose (Sigma-Aldrich), protease inhibitor cocktail (Complete Mini EDTA-free protease inhibitor cocktail, Roche Applied Science, Laval, QC, Canada)) and then homogenized with a glass putter for 1 min. Cell lysates were centrifuged 15 min at 3,000 rpm (4 °C), supernatants were collected and then centrifuged at 38,000 rpm for 1 h30 at 4 °C. Supernatants were harvested and enriched membrane fractions in pellet were resuspended in lysis buffer (150 mM NaCl (ACP), 50 mM Tris–HCl, pH 7.6, 1 % Triton X-100 (Fisher Scientific Ltd.), 0.1 % SDS (Bioshop Canada Inc., Burlington, ON, Canada), protease inhibitor cocktail). Protein concentrations were then measured by Bradford assay and immunoblotting was conducted as described below.
For co-immunoprecipitation assays, ATII cell lysis was performed in buffer 1 (150 mM NaCl, 50 mM Tris–HCl, pH 7.5, 1 % Nonidet P40, and 0.5 % sodium deoxycholate) from the immunoprecipitation kit (protein A, Roche Applied Science) following the manufacturer’s instructions. The lysates were homogenized and isolated by centrifugation. After quantification using the Bradford method, 1–2 mg proteins from the soluble lysate were precleared with 50 μl of 50 % protein A-agarose suspension. The precleared soluble lysates were then incubated for 1–2 h with a rabbit anti-KCa3.1 antibody (APC-064, Alomone Labs, Jerusalem, Israel)  or a mouse anti-β1-integrin antibody (610467, BD Biosciences, Mississauga, ON, Canada). The immunocomplexes were precipitated by overnight incubation at 4 °C with 50 μl of 50 % protein A-agarose suspension. After being washed twice with both buffer 1 (see composition above) and buffer 2 (500 mM NaCl, 50 mM Tris–HCl, pH 7.5, 0.1 % Nonidet P40, 0.05 % sodium deoxycholate) and then once with buffer 3 (10 mM Tris · HCl, pH 7.5, 0.1 % Nonidet P40, 0.05 % sodium deoxycholate), proteins bound to beads were collected by centrifugation and eluted by 30–50 μl of 2X sample buffer (62.5 mM Tris–HCl, pH 6.8, 2 % SDS, 10 % glycerol, 0.2 % bromophenol blue, 4 % β-mercaptoethanol). Control assays, in the absence of lysate or antibodies during IP, have also been performed.
Enriched membrane fraction proteins and immunoprecipitated proteins were then separated by SDS-PAGE and transferred onto nitrocellulose membranes (GE Healthcare, Mississauga, ON, Canada) according to a well-established laboratory protocol [15, 36]. After blocking, the membranes were incubated with anti-KCa3.1 (APC-064, Alomone Labs), anti-β1-integrin (610467, BD Biosciences) or anti-TRPC4 (ACC-018, Alomone Labs) antibodies. After washing, the membranes were incubated with goat anti-rabbit (for KCa3.1 and TRPC4, Cell Signaling Technology, Danvers, MA, USA) and goat anti-mouse (for β1-integrin, Millipore, Etobicoke, ON, Canada) IgG linked to horseradish peroxidase for 1 h. The intensity of each specific band was quantified with ChemiDoc XRS+ Molecular Imager and Image Lab software (Bio-Rad, Mississauga, ON, Canada).
ATII cells were seeded at low confluency on glass coverslips. On day 3, cells were fixed in 4 % paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) in PBS for 20 min and permeabilized with 0.1 % triton X100 in PBS for 10 min at room temperature. After blocking in PBS containing 5 % bovine serum albumin (Fisher Scientific Ltd.) for 1 h30, cells were incubated with specific anti-KCa3.1 and β1-integrin antibodies for 1 h30. Absence of background signal and non-specific staining was verified in control experiments, by omitting primary antibodies. Cells were then incubated with anti-rabbit-CF633 (Sigma-Aldrich, for KCa3.1 detection) or Alexa Fluor 488-conjugated anti-mouse (Life Technologies Inc., for β1-integrin detection) antibodies for 45 min. Slides were finally rinsed and counterstained with DAPI (Sigma-Aldrich). Fluorescent images were captured with an ExiAqua camera (QImaging, Surrey, BC, Canada) connected to an Olympus fluorescence microscope (Olympus, Richmond Hill, ON, Canada).
Small interfering (si)RNA transfections
After isolation, ATII cells in suspension were transfected with a combination of Lipofectamine™ RNAiMAX (Invitrogen) and Universal negative control or KCa3.1 siRNAs (Sigma-Aldrich). Five h after seeding, fresh complete MEM was added onto cells, which were then primary cultured for 3 to 4 days. Efficient transfection (>80 %) was observed in these conditions and KCa3.1 silencing (>70 %) was verified by PCR (see below).
Five μg of total RNA purified from ATII cells with TRIzol reagent (Life Technologies Inc.) was reverse-transcribed into cDNA with MMLV reverse transcriptase (RT, Life Technologies Inc.) in the presence of oligodT primers (Invitrogen, Life Technologies Inc.). cDNAs were amplified with Taq polymerase (Life Technologies Inc.) and specific primers designed from sequences of rat KCa3.1 and TRPC channels: KCa3.1 5′-gctgttcatgactgacaacg-3′ and 5′-catagccaatggtcaggaac-3′, 500-bp product; TRPC1 5′- gattttgggaaatttctaggaatg-3′ and 5′-ctcatgatttgctatcagctgg-3′, 363-bp product; TRPC2 5′- cgttccagtttctcttctggaccat-3′ and 5′- agcatcgtcctcgatcttctgg-3′, 191-bp product; TRPC3 5′- tgatgaggtgaacgaaggtgaactg-3′ and 5′- tgcccacatttgtgccagagtca-3′, 206-bp product; TRPC4 5′- tctgcagatatctctgggaaggatgc-3′ and 5′- aagctttgttcgagcaaatttccattc-3′, 415-bp product; TRPC5 5′- cccggcatgaattcacggag-3′ and 5′- catggtcggcaatgagctggtag-3′, 129-bp product; TRPC6 5′- gagaacataggctatgttctgtatggagtc-3′ and 5′- gccatcatcctcaatttcctgg-3′, 114-bp product; TRPC7 5′- tccctttaacctggtgccgagtc-3′ and 5′- ttcagcatgcccatttccagg-3′, 129-bp product. Primer pairs were designed in distinct exons to avoid genomic DNA amplification. RT-PCR amplification was undertaken according to a well-established laboratory protocol [35, 36, 44]. PCR products were normalized with the β-actin signal for each cDNA sample (β-actin 5′-ctaaggccaaccgtgaaaag-3′ and 5′- gccatctcttgctcgaagtc-3′, 311-bp product). The RT-PCR products were finally separated on agarose gels, stained with SYBR Safe (Life Technologies Inc.). Signals were detected by Typhoon Gel Imager and analyzed by ImageQuant software (Molecular Dynamics, Baie d’Urfé, QC, Canada) [35, 36, 44].
Fura-2 calcium imaging
Intracellular calcium concentration ([Ca2+]i) was monitored using the fluorescent dye Fura-2. Briefly, ATII cells cultured on coverslips were loaded (1 h15 at 37 °C) with 5 μM Fura-2-AM (Life Technologies Inc.) in MEM containing 0.04 % pluronic F127 (Life technologies Inc.) followed by a 45 min de-esterification in MEM. Coverslips with loaded cells were then mounted in the imaging chamber on the stage of an inverted microscope (Nikon TE300, Mississauga, ON, Canada) and bathed with a physiological saline solution (PSS, 140 mM NaCl, 5 mM KCl, 1 mM MgCl2, 2 mM CaCl2, 11.1 mM glucose, 10 mM HEPES, pH 7.4). To measure the Ca2+ permeability through ATII cell plasma membranes, PSS was replaced by the same solution without Ca2+ and supplemented with 1 mM EGTA (PSS 0 Ca2+). Cells were then alternately excited at 340 and 380 nm with a high-pressure mercury lamp via interference filters (Chroma Technology, Brattleboro, VT, USA) mounted on a filter wheel (Sutter Lamba 10-C, Sutter Instrument, Novato, CA, USA) with a dichroic mirror (510/540 nm, Chroma Technology). Fluorescence images were recorded at 5-s intervals by digital camera using Metafluor software (Molecular Devices, Guelph, ON, Canada) and analyzed with Metafluor Analysis (Molecular Devices). Fura-2 fluorescence measurements (corresponding to the mean fluorescence values of 30 cells/field) are presented as [Ca2+]i, which was calculated accordingly to the Grynkiewicz equation , using a Kd of 225 nM. Rmax was determined by the addition of digitonin (4 μM, Sigma-Aldrich) to PSS (2 mM Ca2+) and Rmin by subsequent addition of ethylene glycol-bis ([β-amino-ethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA) at a final concentration of 20 mM.
The data are presented as mean ± standard error of the mean (SEM). Statistical analyses were performed with GraphPad Prism version 5 for Windows (GraphPad software, San Diego, CA, USA) using Wilcoxon signed rank test and one sample sign test. Two-way Anova tests were performed with IBM SPSS Statistics 22 (IBM Corp., Armonk, NY). Differences were considered significant when p < 0.05.
Involvement of KCa3.1 in ATII epithelial repair on fibronectin matrix
We also observed a dose-dependent reduction of fibronectin-stimulated wound-healing rates after KCa3.1 inhibition (15 and 33 % inhibition in the presence of 5 and 10 μM TRAM-34 respectively, Fig. 1b). In a previous study , we demonstrated an involvement of two other types of K+ channels, i.e. KvLQT1 and KATP, during alveolar repair which did not required fibronectin coating. We now verified that the wound healing rate on fibronectin coating (47.0 ± 3.8 × 103 μm2/h) was also significantly reduced in the presence of KvLQT1 and KATP inhibitors (i.e., clofilium (5 μM) and glibenclamide (10 μM), 32.1 ± 5.0 × 103 μm2/h, p < 0.04, not shown). Moreover, the combination of TRAM-34 (20 μM), with clofilium and glibenclamide, elicited an additional inhibitory effect of the repair rates on fibronectin (24.4 ± 4.4 × 103 μm2/h, p < 0.04, not shown).
Our data point to an involvement of KCa3.1 channels in the regulation of ATII wound repair in the presence of a fibronectin matrix. We then undertook complementary experiments to further assess the role of KCa3.1 in two crucial processes of epithelial repair, i.e. cell proliferation and migration.
Impact of KCa3.1 inhibition on ATII cell growth
Involvement of KCa3.1 in ATII cell motility
Impact of KCa3.1 silencing on wound healing, cell proliferation and migration
Wound healing stimulation by KCa3.1 activation
Relationship between KCa3.1 channels and β1-integrin
Based on our data indicating a cooperative role played by KCa3.1 and fibronectin matrix in alveolar wound repair, we then decided to further investigate a possible relationship between the KCa3.1 channel and the β1-integrin, a receptor and signal transducer of fibronectin .
We then assessed a possible assembly of these two proteins using co-immunoprecipitation assays. Expression of β1-integrin and KCa3.1 proteins was first confirmed by western blotting on ATII cell extracts (Fig. 6b, lane 1, « total lysate »), where doublets of 120–105 (mature and immature β1-integrin proteins, IB: β1-integrin) and 51 kDa (IB: KCa3.1) bands were detected using anti-β1-integrin and anti-KCa3.1 antibodies, respectively. Interestingly, KCa3.1 proteins was also detected after immunoprecipitation (IP) using anti-β1-integrin antibody (Fig. 6b, lane 2, « β1-integrin IP », IB: KCa3.1). We also detected β1-integrin proteins, after KCa3.1 immunoprecipitation (Fig. 6b, lane 3, « KCa3.1 IP », IB: β-integrin). This result thus showed for the first time that KCa3.1 could form an immunoprecipitable complex with the β-1 integrin in the ATII cells.
The expression levels of KCa3.1 and β1-integrin proteins in enriched membrane fractions of ATII cells were then compared in the presence or absence of fibronectin coating. We noted that ATII cells grown in the presence of fibronectin matrix expressed a higher level of the KCa3.1 and β1-integrin proteins in their membrane fractions (~50 % and 80 % enhancement, respectively) compared to cells cultured in the absence of coating (Fig. 6c). This observation reinforced the hypothesis of a relationship between β1-integrin and KCa3.1.
Involvement of calcium transport in fibronectin-stimulated ATII wound repair
A final series of experiments was undertaken to examine the involvement of TRPC4 channels, using the specific inhibitor ML-204 , in fibronectin-stimulated alveolar wound repair. As previously observed after KCa3.1 inhibition with TRAM-34 (Fig. 1b), blocking of TRPC4 channels with ML-204 (Fig. 8d, ML-204) did not significantly reduce the wound-healing rates of ATII monolayers in the absence of coating, whereas ML-204 significantly decreased the rates on fibronectin matrix (fibronectin + ML-204). Finally, an additive inhibitory effect of TRAM-34 on wound-healing rates was observed, suggesting a complementary role played by KCa3.1 and TRPC4 channels during ATII epithelial repair on fibronectin (Fig. 8d, fibronectine + ML-204 + TRAM-34).
The observed increases in ATII wound-healing (Fig. 1) and cell migration (Fig. 3) rates on fibronectin confirm importance of this extracellular matrix component in alveolar repair, in agreement with previous studies. Indeed, Kim et al. demonstrated using Boyden chamber assays that alveolar epithelial cell migration was increased in the presence of either soluble or coated fibronectin . A role for this extracellular matrix component has also been shown in other epithelial tissues, including in the cornea .
One of our previous studies  demonstrated that KvLQT1 and KATP channels actively participate in the control of ATII wound healing in the absence of fibronectin coating, whereas our present data (Figs. 1 and 4b) indicate that KCa3.1 did not affect the wound repair rates in these conditions. Interestingly, both KCa3.1 pharmacological inhibition and molecular silencing impaired fibronectin-stimulated ATII wound-repair rates (Figs. 1 and 4b). This discrepancy could be explained by variable basal activity of KvLQT1, KATP and KCa3.1 channels in ATII cells. Indeed, it is possible that KCa3.1 channels require a signal, through β1-integrin activation in presence of fibronectin, to participate in ATII wound-healing processes, whereas KvLQT1 and KATP channels may exhibit sufficient basal activity to regulate alveolar epithelial repair even in absence of coating. In agreement with this hypothesis our data indicated an additive effect after concomitant inhibition of KvLQT1, KATP and KCa3.1 channels on fibronectin matrix. It could also be postulated that the TRAM-34-sensitivity on fibronectin coating could be due to the observed increased expression (Fig. 6c) of KCa3.1/integrin proteins, which cooperate to regulate alveolar repair, migration and proliferation in this condition. A role for KCa3.1 channels in the control of both ATII cell proliferation and migration on fibronectin (Fig. 4) was also confirmed by the siRNA approach. Such an involvement of KCa3.1 in cell motility [52–55] or proliferation [56, 57] had previously been established in other cell types.
The observation that KCa3.1 function in alveolar repair is dependent on the presence of fibronectin suggests a potential link between KCa3.1 and extracellular matrix receptors, such as integrins. In agreement with this hypothesis, our immunofluorescence and co-immunoprecipation experiments (Fig. 6) demonstrate for the first time a cellular co-distribution and a potential physical link between KCa3.1 and β1-integrin in ATII cells. Although such observations have never been reported before in native epithelial cells, physical or functional interactions between other types of K+ channels (e.g. BKCa, Kv1.3, hERG, Kir4.2) and integrins have already been reported in other cell types [30–33]. Levite et al., for example, reported a physical association between Kv1.3 channels and β1-integrin, detected by co-immunoprecipitation assays, in human T cells . The same approach was undertaken in a neuroblastoma cell line to identify a link between hERG1 channel and β1-integrin, which were also co-localized .
In the present study, we observed a significant increase in β1-integrin and KCa3.1 expression in the membrane fraction of ATII cells cultured on fibronectin coating (Fig. 6c). Although our data did not allow us to define which cellular mechanism could explain this observed up-regulation, it has been proposed in other studies that protein phosphorylation, Ca2+ signalling or G proteins could be responsible for the modulation of K+ channel properties by integrins [9, 58]. In agreement with our results, it has been also shown that the level of hERG channel co-immunoprecipitated with β1-integrin is increased after β1-integrin activation .
Due to the Ca2+ sensitivity of KCa3.1 channels, the levels of [Ca2+]i in ATII cells in the absence or presence of a fibronectin matrix were also analyzed. Our data showed that [Ca2+]i is significantly higher in the presence of fibronectin (Fig. 7). This result is in agreement with a study by Sjaastad et al.,  showing that integrin activation in MDCK cells, using microbeads coated with RGD motif, was associated with [Ca2+]i increase, most probably through a Ca2+ influx. Our Fura-2 assays also indicated that membrane Ca2+ permeability in ATII cells was enhanced in the presence of fibronectin. In an attempt to define the identity of the membrane Ca2+ channel(s) that may be involved in that response, we decided to focus on the TRPC family, because TRPC3 channel has already been shown to facilitate the KCa3.1 channel activation . Although we detected all members of the TRPC family (TRPC1-7, , Fig. 8) in ATII cells, only TRPC4 mRNA expression was significantly enhanced in the presence of fibronectin. Moreover, analysis of protein expression in membrane fraction revealed that TRPC4 levels, similarly to KCa3.1 and β-1 integrin, were significantly enhanced in ATII cells cultured on fibronectin coating (Fig. 8c). The signalling pathways responsible for TRPC4 up-regulation were not elucidated in our study; however, it has previously been demonstrated that TRPC4 membrane translocation was dependent of Src phosphorylation after EGF stimulation ; a phosphorylation pathway also known in the regulation of ion channels by integrin [9, 58]. The relative contribution of TRPC4, as well as other types of Ca2+ channels, including voltage-dependent and independent Ca2+ channels to the complex Ca2+ signal during wound repair or after changes in integrin and K+ channel activation has not been evaluated in that study but would deserve further investigation.
Finally, we observed for the first time that TRPC4 channels are involved in ATII wound healing. In fact, we measured a 3-fold stronger inhibition of ATII wound repair rates with the TRPC4 inhibitor ML204 on the fibronectin matrix than in the absence of coating. This effect is in agreement with the observed increased TRPC expression on fibronectin coating. Although a function of TRPC4 channels in alveolar repair has never been reported before, other members of the TRPC family have already been described as regulators of cell migration in non-tumoural cells (TRPC1 and TRPC3 in intestinal epithelial cells or monocytes respectively [61, 62]) or in lung cancer cells (TRPC1, 3, 4 and 6 ). Furthermore, we observed that the combination of KCa3.1 and TRPC4 inhibitors led to an additive inhibitory effect (Fig. 8d), suggesting that these two channels are complementary partners in the control of ATII wound healing on fibronectin.
As described above, our data using KCa3.1 inhibition or silencing demonstrated that KCa3.1 may act as a regulator of the ATII wound-healing repair in vitro. Interestingly, we also showed that the ATII wound-healing rates were improved in the presence of the KCa activator 1-EBIO (Fig. 5). Based on this evidence, it may be postulated that KCa3.1 could play an important role in the alveolar epithelial repair after injury and may thus be identified as a potential therapeutic target for alveolar epithelial repair after acute lung injury in vivo. Such a strategy remains to be further investigated, however.
Our data demonstrate for the first time the complementary roles of fibronectin matrix, β-1 integrin, KCa3.1 and TRPC4 channels in the regulation of alveolar epithelial repair processes (Fig. 9). Moreover, our study highlights an improvement in ATII wound-healing after treatment with 1-EBIO, suggesting use of KCa3.1 activators as potential therapeutic strategy to favor the resolution of acute lung injury in vivo.
This work was supported by Canadian Institutes of Health Research (Grant MOP-111054), the Natural Sciences and Engineering Research Council of Canada (Grant RGPIN/312177-2011), a scholarship from the Centre de Recherche du Centre Hospitalier de l’Université de Montréal (CRCHUM) and Université de Montréal (E. Brochiero), a fellowship from the Canadian Institutes of Health Research training program of the Respiratory Health Network and Fonds de la Recherche du Québec en Santé (A. Girault), a fellowship of the FRQ-S (A. Girault), a studenship of the Faculté de Médecine, Université de Montréal (J. Chebli). The authors acknowledge logistical assistance from the Research Support Office at the Centre de Recherche du Centre Hospitalier de l’Université de Montréal (CRCHUM) as well as advice from Dr Lise Coderre (for data analysis).
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Berthiaume Y, Lesur O, Dagenais A. Treatment of adult respiratory distress syndrome: plea for rescue therapy of the alveolar epithelium. Thorax. 1999;54:150–60.View ArticlePubMedPubMed CentralGoogle Scholar
- Ware LB, Matthay MA. The acute respiratory distress syndrome. N Engl J Med. 2000;342:1334–49.View ArticlePubMedGoogle Scholar
- Shimabukuro DW, Sawa T, Gropper MA. Injury and repair in lung and airways. Crit Care Med. 2003;31:S524–31.View ArticlePubMedGoogle Scholar
- Budinger GR, Sznajder JI. The alveolar-epithelial barrier: a target for potential therapy. Clin Chest Med. 2006;27:655–69.View ArticlePubMedGoogle Scholar
- Crosby LM, Waters CM. Epithelial repair mechanisms in the lung. Am J Physiol Lung Cell Mol Physiol. 2010;298:L715–31.View ArticlePubMedPubMed CentralGoogle Scholar
- Iizuka M, Konno S. Wound healing of intestinal epithelial cells. World J Gastroenterol. 2011;17:2161–71.View ArticlePubMedPubMed CentralGoogle Scholar
- Coraux C, Roux J, Jolly T, Birembaut P. Epithelial cell-extracellular matrix interactions and stem cells in airway epithelial regeneration. Proc Am Thorac Soc. 2008;5:689–94.View ArticlePubMedGoogle Scholar
- Sheppard D. Epithelial integrins. Bioessays. 1996;18:655–60.View ArticlePubMedGoogle Scholar
- Arcangeli A, Becchetti A. Complex functional interaction between integrin receptors and ion channels. Trends Cell Biol. 2006;16:631–9.View ArticlePubMedGoogle Scholar
- Cabodi S, Di Stefano P, Leal MP, Tinnirello A, Bisaro B, Morello V, et al. Integrins and signal transduction. Adv Exp Med Biol. 2010;674:43–54.View ArticlePubMedGoogle Scholar
- Herard AL, Pierrot D, Hinnrasky J, Kaplan H, Sheppard D, Puchelle E, et al. Fibronectin and its alpha 5 beta 1-integrin receptor are involved in the wound-repair process of airway epithelium. Am J Physiol. 1996;271:L726–33.PubMedGoogle Scholar
- Kim HJ, Henke CA, Savik SK, Ingbar DH. Integrin mediation of alveolar epithelial cell migration on fibronectin and type I collagen. Am J Physiol. 1997;273:L134–41.PubMedGoogle Scholar
- Morales MM, Pires-Neto RC, Inforsato N, Lancas T, da Silva LF, Saldiva PH, et al. Small airway remodeling in acute respiratory distress syndrome: a study in autopsy lung tissue. Crit Care. 2011;15:R4.View ArticlePubMedPubMed CentralGoogle Scholar
- Girault A, Brochiero E. Evidence of K+ channel function in epithelial cell migration, proliferation and repair. Am J Physiol Cell Physiol. 2014;306:C307–19.View ArticlePubMedGoogle Scholar
- Trinh NT, Prive A, Kheir L, Bourret JC, Hijazi T, Amraei MG, et al. Involvement of KATP and KvLQT1 K+ channels in EGF-stimulated alveolar epithelial cell repair processes. Am J Physiol Lung Cell Mol Physiol. 2007;293:L870–82.View ArticlePubMedGoogle Scholar
- Trinh NT, Prive A, Maille E, Noel J, Brochiero E. EGF and K+ channel activity control normal and cystic fibrosis bronchial epithelia repair. Am J Physiol Lung Cell Mol Physiol. 2008;295:L866–80.View ArticlePubMedGoogle Scholar
- Roderick C, Reinach PS, Wang L, Lu L. Modulation of rabbit corneal epithelial cell proliferation by growth factor-regulated K(+) channel activity. J Membr Biol. 2003;196:41–50.View ArticlePubMedGoogle Scholar
- Schwingshackl A, Teng B, Ghosh M, West AN, Makena P, Gorantla V, et al. Regulation and function of the two-pore-domain (K2P) potassium channel Trek-1 in alveolar epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2012;302:L93–L102.View ArticlePubMedGoogle Scholar
- Rao JN, Platoshyn O, Li L, Guo X, Golovina VA, Yuan JX, et al. Activation of K(+) channels and increased migration of differentiated intestinal epithelial cells after wounding. Am J Physiol Cell Physiol. 2002;282:C885–98.View ArticlePubMedGoogle Scholar
- Rampino T, Gregorini M, Guidetti C, Broggini M, Marchini S, Bonomi R, et al. KCNA1 and TRPC6 ion channels and NHE1 exchanger operate the biological outcome of HGF/scatter factor in renal tubular cells. Growth Factors. 2007;25:382–91.View ArticlePubMedGoogle Scholar
- Wu WK, Li GR, Wong TM, Wang JY, Yu L, Cho CH. Involvement of voltage-gated K+ and Na + channels in gastric epithelial cell migration. Mol Cell Biochem. 2008;308:219–26.View ArticlePubMedGoogle Scholar
- Schwab A, Wojnowski L, Gabriel K, Oberleithner H. Oscillating activity of a Ca(2+)-sensitive K+ channel. A prerequisite for migration of transformed Madin-Darby canine kidney focus cells. J Clin Invest. 1994;93:1631–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Wang JY, Wang J, Golovina VA, Li L, Platoshyn O, Yuan JX. Role of K(+) channel expression in polyamine-dependent intestinal epithelial cell migration. Am J Physiol Cell Physiol. 2000;278:C303–14.PubMedGoogle Scholar
- Buchanan PJ, McNally P, Harvey BJ, Urbach V. Lipoxin A4 mediated KATP potassium channel activation results in cystic fibrosis airway epithelial repair. Am J Physiol Lung Cell Mol Physiol. 2013;305:L193–201.View ArticlePubMedGoogle Scholar
- Potier M, Tran TA, Chantome A, Girault A, Joulin V, Bougnoux P, et al. Altered SK3/KCa2.3-mediated migration in adenomatous polyposis coli (Apc) mutated mouse colon epithelial cells. Biochem Biophys Res Commun. 2010;397:42–7.View ArticlePubMedGoogle Scholar
- Wei JF, Wei L, Zhou X, Lu ZY, Francis K, Hu XY, et al. Formation of Kv2.1-FAK complex as a mechanism of FAK activation, cell polarization and enhanced motility. J Cell Physiol. 2008;217:544–57.View ArticlePubMedPubMed CentralGoogle Scholar
- Cowley EA, Linsdell P. Characterization of basolateral K+ channels underlying anion secretion in the human airway cell line Calu-3. J Physiol. 2002;538:747–57.View ArticlePubMedPubMed CentralGoogle Scholar
- Mall M, Gonska T, Thomas J, Schreiber R, Seydewitz HH, Kuehr J, et al. Modulation of Ca2 + −activated Cl- secretion by basolateral K+ channels in human normal and cystic fibrosis airway epithelia. Pediatr Res. 2003;53:608–18.View ArticlePubMedGoogle Scholar
- Jin M, Defoe DM, Wondergem R. Hepatocyte growth factor/scatter factor stimulates Ca2 + −activated membrane K+ current and migration of MDCK II cells. J Membr Biol. 2003;191:77–86.View ArticlePubMedGoogle Scholar
- Cherubini A, Hofmann G, Pillozzi S, Guasti L, Crociani O, Cilia E, et al. Human ether-a-go-go-related gene 1 channels are physically linked to beta1 integrins and modulate adhesion-dependent signaling. Mol Biol Cell. 2005;16:2972–83.View ArticlePubMedPubMed CentralGoogle Scholar
- deHart GW, Jin T, McCloskey DE, Pegg AE, Sheppard D. The alpha9beta1 integrin enhances cell migration by polyamine-mediated modulation of an inward-rectifier potassium channel. Proc Natl Acad Sci U S A. 2008;105:7188–93.View ArticlePubMedPubMed CentralGoogle Scholar
- Levite M, Cahalon L, Peretz A, Hershkoviz R, Sobko A, Ariel A, et al. Extracellular K(+) and opening of voltage-gated potassium channels activate T cell integrin function: physical and functional association between Kv1.3 channels and beta1 integrins. J Exp Med. 2000;191:1167–76.View ArticlePubMedPubMed CentralGoogle Scholar
- Yang Y, Wu X, Gui P, Wu J, Sheng JZ, Ling S, et al. Alpha5beta1 integrin engagement increases large conductance, Ca2 + −activated K+ channel current and Ca2+ sensitivity through c-src-mediated channel phosphorylation. J Biol Chem. 2010;285:131–41.View ArticlePubMedGoogle Scholar
- Leroy C, Dagenais A, Berthiaume Y, Brochiero E. Molecular identity and function in transepithelial transport of K(ATP) channels in alveolar epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2004;286:L1027–37.View ArticlePubMedGoogle Scholar
- Leroy C, Prive A, Bourret JC, Berthiaume Y, Ferraro P, Brochiero E. Regulation of ENaC and CFTR expression with K+ channel modulators and effect on fluid absorption across alveolar epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2006;291:L1207–19.View ArticlePubMedGoogle Scholar
- Bardou O, Prive A, Migneault F, Roy-Camille K, Dagenais A, Berthiaume Y, et al. K(+) channels regulate ENaC expression via changes in promoter activity and control fluid clearance in alveolar epithelial cells. Biochim Biophys Acta. 1818;2012:1682–90.Google Scholar
- Dobbs LG, Gonzalez R, Williams MC. An improved method for isolating type II cells in high yield and purity. Am Rev Respir Dis. 1986;134:141–5.PubMedGoogle Scholar
- Brochiero E, Dagenais A, Prive A, Berthiaume Y, Grygorczyk R. Evidence of a functional CFTR Cl(−) channel in adult alveolar epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2004;287:L382–92.View ArticlePubMedGoogle Scholar
- Girault A, Prive A, Trinh NT, Bardou O, Ferraro P, Joubert P, et al. Identification of KvLQT1 K+ channels as new regulators of non-small cell lung cancer cell proliferation and migration. Int J Oncol. 2014;44:838–48.PubMedGoogle Scholar
- Maille E, Trinh NT, Prive A, Bilodeau C, Bissonnette E, Grandvaux N, et al. Regulation of normal and cystic fibrosis airway epithelial repair processes by TNF-alpha after injury. Am J Physiol Lung Cell Mol Physiol. 2011;301:L945–55.View ArticlePubMedGoogle Scholar
- Trinh NT, Bardou O, Prive A, Maille E, Adam D, Lingée S, et al. Improvement of defective cystic fibrosis airway epithelial wound repair after CFTR rescue. Eur Respir J. 2012;40:1390–400.View ArticlePubMedGoogle Scholar
- Bilodeau C, Bardou O, Maille E, Berthiaume Y, Brochiero E. Deleterious impact of hyperglycemia on cystic fibrosis airway ion transport and epithelial repair. J Cyst Fibros. 2015.Google Scholar
- Klein H, Garneau L, Trinh NT, Prive A, Dionne F, Goupil E, et al. Inhibition of the KCa3.1 channels by AMP-activated protein kinase in human airway epithelial cells. Am J Physiol Cell Physiol. 2009;296:C285–95.View ArticlePubMedGoogle Scholar
- Dagenais A, Frechette R, Clermont ME, Masse C, Prive A, Brochiero E, et al. Dexamethasone inhibits the action of TNF on ENaC expression and activity. Am J Physiol Lung Cell Mol Physiol. 2006;291:L1220–31.View ArticlePubMedGoogle Scholar
- Grynkiewicz G, Poenie M, Tsien RY. A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem. 1985;260:3440–50.PubMedGoogle Scholar
- Wei AD, Gutman GA, Aldrich R, Chandy KG, Grissmer S, Wulff H. International Union of Pharmacology. LII. Nomenclature and molecular relationships of calcium-activated potassium channels. Pharmacol Rev. 2005;57:463–72.View ArticlePubMedGoogle Scholar
- Venkatachalam K, Montell C. TRP channels. Annu Rev Biochem. 2007;76:387–417.View ArticlePubMedPubMed CentralGoogle Scholar
- Nilius B, Szallasi A. Transient receptor potential channels as drug targets: from the science of basic research to the art of medicine. Pharmacol Rev. 2014;66:676–814.View ArticlePubMedGoogle Scholar
- Senadheera S, Kim Y, Grayson TH, Toemoe S, Kochukov MY, Abramowitz J, et al. Transient receptor potential canonical type 3 channels facilitate endothelium-derived hyperpolarization-mediated resistance artery vasodilator activity. Cardiovasc Res. 2012;95:439–47.View ArticlePubMedPubMed CentralGoogle Scholar
- Miller M, Shi J, Zhu Y, Kustov M, Tian JB, Stevens A, et al. Identification of ML204, a novel potent antagonist that selectively modulates native TRPC4/C5 ion channels. J Biol Chem. 2011;286:33436–46.View ArticlePubMedPubMed CentralGoogle Scholar
- Nishida T, Nakagawa S, Awata T, Ohashi Y, Watanabe K, Manabe R. Fibronectin promotes epithelial migration of cultured rabbit cornea in situ. J Cell Biol. 1983;97:1653–7.View ArticlePubMedGoogle Scholar
- Cruse G, Singh SR, Duffy SM, Doe C, Saunders R, Brightling CE, et al. Functional KCa3.1 K+ channels are required for human fibrocyte migration. J Allergy Clin Immunol. 2011;128:1303–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Schwab A, Schuricht B, Seeger P, Reinhardt J, Dartsch PC. Migration of transformed renal epithelial cells is regulated by K+ channel modulation of actin cytoskeleton and cell volume. Pflugers Arch. 1999;438:330–7.View ArticlePubMedGoogle Scholar
- Chimote AA, Hajdu P, Kucher V, Boiko N, Kuras Z, Szilagyi O, et al. Selective inhibition of KCa3.1 channels mediates adenosine regulation of the motility of human T cells. J Immunol. 2013;191:6273–80.View ArticlePubMedPubMed CentralGoogle Scholar
- D'Alessandro G, Catalano M, Sciaccaluga M, Chece G, Cipriani R, Rosito M, et al. KCa3.1 channels are involved in the infiltrative behavior of glioblastoma in vivo. Cell Death Dis. 2013;4:e773.View ArticlePubMedPubMed CentralGoogle Scholar
- Grossinger EM, Weiss L, Zierler S, Rebhandl S, Krenn PW, Hinterseer E, et al. Targeting proliferation of chronic lymphocytic leukemia (CLL) cells through KCa3.1 blockade. Leukemia. 2014;28:954–8.View ArticlePubMedGoogle Scholar
- Tao R, Lau CP, Tse HF, Li GR. Regulation of cell proliferation by intermediate-conductance Ca2 + −activated potassium and volume-sensitive chloride channels in mouse mesenchymal stem cells. Am J Physiol Cell Physiol. 2008;295:C1409–16.View ArticlePubMedPubMed CentralGoogle Scholar
- Davis MJ, Wu X, Nurkiewicz TR, Kawasaki J, Gui P, Hill MA, et al. Regulation of ion channels by integrins. Cell Biochem Biophys. 2002;36:41–66.View ArticlePubMedGoogle Scholar
- Sjaastad MD, Lewis RS, Nelson WJ. Mechanisms of integrin-mediated calcium signaling in MDCK cells: regulation of adhesion by IP3- and store-independent calcium influx. Mol Biol Cell. 1996;7:1025–41.View ArticlePubMedPubMed CentralGoogle Scholar
- Odell AF, Scott JL, Van Helden DF. Epidermal growth factor induces tyrosine phosphorylation, membrane insertion, and activation of transient receptor potential channel 4. J Biol Chem. 2005;280:37974–87.View ArticlePubMedGoogle Scholar
- Rao JN, Platoshyn O, Golovina VA, Liu L, Zou T, Marasa BS, et al. TRPC1 functions as a store-operated Ca2+ channel in intestinal epithelial cells and regulates early mucosal restitution after wounding. Am J Physiol Gastrointest Liver Physiol. 2006;290:G782–92.View ArticlePubMedGoogle Scholar
- Zhao Z, Ni Y, Chen J, Zhong J, Yu H, Xu X, et al. Increased migration of monocytes in essential hypertension is associated with increased transient receptor potential channel canonical type 3 channels. PLoS One. 2012;7:e32628.View ArticlePubMedPubMed CentralGoogle Scholar
- Jiang HN, Zeng B, Zhang Y, Daskoulidou N, Fan H, Qu JM, et al. Involvement of TRPC channels in lung cancer cell differentiation and the correlation analysis in human non-small cell lung cancer. PLoS One. 2013;8:e67637.View ArticlePubMedPubMed CentralGoogle Scholar