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Fig. 3 | Respiratory Research

Fig. 3

From: Complementary roles of KCa3.1 channels and β1-integrin during alveolar epithelial repair

Fig. 3

Impact of KCa3.1 inhibition on ATII cell motility. ATII cell migration was evaluated using Boyden-type assays (a) and single cell tracking in live time-lapse videomicroscopy (b). The number of migrating ATII cells were measured over an 18-h period in the absence (0) or presence of 5, 10 or 20 μM TRAM-34 and compared in the absence (a, no fibronectin coating, left panel) or presence of fibronectin (a, right panel) (5 fields/insert, 3 inserts/condition, n = 4-6). 2D cell migration rates (b) were evaluated by ATII single-cell tracking (~15 cells/field, 2 fields/condition/experiment, n = 6) over a 24-h period in the absence (0) or presence of TRAM-34 (5, 10 and 20 μM). Representative trajectories of ATII cells in the absence or presence of a fibronectin matrix, in control condition and in the presence of 20 μM TRAM-34 are presented in the top panel (b). Colors indicate 0-h (blue) to 24-h (red) time points. *p < 0.05

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