Budesonide and formoterol effects on rhinovirus replication and epithelial cell cytokine responses
© Bochkov et al.; licensee BioMed Central Ltd. 2013
Received: 8 May 2013
Accepted: 28 September 2013
Published: 4 October 2013
Combination therapy with budesonide and formoterol reduces exacerbations of asthma, which are closely associated with human rhinovirus (RV) infections in both children and adults. These data suggest that budesonide and formoterol inhibit virus-induced inflammatory responses of airway epithelial cells.
To test this hypothesis, bronchial epithelial (BE) cells were obtained from airway brushings of 8 subjects with moderate-to-severe allergic asthma and 9 with neither asthma nor respiratory allergies. Cultured BE cells were incubated for 24 hours with budesonide (1.77 μM), formoterol (0.1 μM), both, or neither, and then inoculated with RV-16 (5×106 plaque forming units [PFU]/mL). After 24 hours, viral replication (RV RNA), cytokine secretion (CXCL8, CXCL10, TNFα, IFN-β, IL-28) and mRNA expression (CXCL8, CXCL10, TNF, IFNB1, IL28A&B) were analyzed.
RV infection induced CXCL10 protein secretion and IFNB1 and IL28 mRNA expression. Drug treatments significantly inhibited secretion of CXCL10 in mock-infected, but not RV-infected, BE cells, and inhibited secretion of TNFα under both conditions. Neither budesonide nor formoterol, alone or in combination, significantly affected viral replication, nor did they inhibit RV-induced upregulation of IFNB1 and IL28 mRNA. Overall, RV replication was positively related to CXCL10 secretion and induction of IFNB1 and IL28 mRNA, but the positive relationship between RV RNA and CXCL10 secretion was stronger in normal subjects than in subjects with asthma.
Budesonide and formoterol can inhibit BE cell inflammatory responses in vitro without interfering with viral replication or production of interferons. These effects could potentially contribute to beneficial effects of budesonide/formoterol combination therapy in preventing RV-induced asthma exacerbations.
KeywordsBudesonide Formoterol Human rhinovirus Bronchial epithelial cells Asthma
Infections with human rhinoviruses (RVs) are commonly associated with increased symptoms and acute exacerbations of asthma that require treatment in the emergency department or hospitalization. In fact, RV infections are more often detected during acute exacerbations than any other respiratory virus [1, 2]. This close association has led to research efforts directed at identifying treatment to prevent RV-induced exacerbations of asthma.
Several medications have shown efficacy for prevention of asthma exacerbations in general. Combination therapy with an inhaled corticosteroid (ICS) together with long-acting beta agonist (LABA) is particularly effective at preventing exacerbations [3, 4]. Results of clinical studies also suggest that both ICS and combination medications prevent exacerbations during the common cold season, and exacerbations that are associated with symptoms of the common cold [5, 6].
Defining mechanisms of these effects is important to improving the efficacy of medications that prevent exacerbations, and several mechanisms have been proposed. First, RVs initiate infections by first binding to cell surface receptors on airway epithelial cells. Notably, ICS can downregulate in vitro expression of intercellular adhesion molecule 1 (ICAM-1), which is the cellular receptor for 89 of the 100 canonical RV-A and RV-B types [7, 8]; however, glucocorticoid treatment did not prevent the increased ICAM-1 expression after experimental RV-16 infection . Second, RVs cause illnesses in part by inducing release of pro-inflammatory chemokines and cytokines from infected epithelial cells. These factors include chemoattractants for neutrophils (e.g. CXCL8), mononuclear cells and T cells (e.g. CXCL10), and cytokines such as TNFα that can potentiate inflammatory cell functions [10–13]. ICS can inhibit the RV-induced production of both pro-inflammatory mediators  and type I interferons (e.g. IFN-α) that have antiviral effects . Clinical studies have shown evidence of this effect in vivo in that either systemic or topical corticosteroids were associated with increased RV replication after experimental inoculation with virus; however, cold severity was not affected .
We hypothesized that the combination of budesonide and formoterol would inhibit virus-induced inflammatory responses of primary airway epithelial cells in vitro. To test this hypothesis, we conducted a series of experiments with bronchial epithelial (BE) cells that were obtained from subjects with or without allergic asthma. As a secondary objective, we compared viral replication and RV-induced responses in cells from subjects with and without allergic asthma.
FEV1 % predicted
Allergen Skin Test
Bronchial epithelial cells
BE cells from bronchial brushings were cultured as described previously [11, 18]. Briefly, the cells were removed from the brush by scraping and vigorous agitation in Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12, Life Technologies, Grand Island, NY), and then placed in tissue culture in collagen coated plasticware at 37°C (5% CO2) in Bronchial Epithelial Growth Medium (BEGM, Lonza, Walkersville, MD) in 25 cm2 flasks (passage 0), then expanded in 75 cm2 flasks (passage 1), and finally seeded in 12-well plates (passage 2). Purified and concentrated RV-16  was diluted in BEGM with a reduced concentration (10-8 M) of hydrocortisone (low Hc BEGM) just before infection. Stock solutions of budesonide (anhydrous) and formoterol fumarate (dihydrate) were prepared in DMSO at a concentration of 10 mM, subsequent dilutions were made in low Hc BEGM (final concentration of DMSO applied to cells was ≤ 0.01%). When the cell monolayers (duplicate wells) from each study subject (passage 2) were approximately 70-80% confluent, they were pre-treated for 24 h with budesonide (1.77 μM), formoterol (0.1 μM), both (at recommended therapeutic ratio of 17.7:1), or neither (0.01% DMSO drug vehicle), and then either infected with 0.25 mL of RV-16 (5×106 plaque forming units [PFU]/mL), or mock-infected with medium alone for 2 h at 34°C. Cell monolayers were then washed three times with PBS before adding fresh low Hc BEGM. After 24 h incubation at 34°C (5% CO2), cell supernatants were collected, the monolayers were washed with phosphate buffered saline and lysed by adding TRIzol Reagent (Invitrogen, Carlsbad, CA). Supernatant and cell lysate samples were stored in microcentrifuge tubes at -80°C until RNA extraction and cytokine analysis.
The major receptor group RV-16 was chosen for this study because major group viruses replicate well in cultured BE cells without causing marked cytopathic effects , and thus is a good model of in vivo infections . Stocks of virus were grown in HeLa cells and purified by sucrose gradient centrifugation as described previously .
Measurement of viral and cellular RNAs
Real-time PCR primers used for mRNA quantification
Amplicon size (bp)
Measurement of cytokine proteins
CXCL8, CXCL10, TNFα and IL-28 concentrations were assessed using human Cytokine / Chemokine Milliplex map kits (Millipore, Temecula, CA) according to the manufacturer’s instructions. Luminex® 100 instrument (Luminex Corporation, Austin, TX) was used to run plates and generate quantitative data. Sensitivity of the assays (minimum detectable concentration) was 0.3 pg/mL (CXCL8), 2.2 pg/mL (CXCL10), 7.9 pg/mL (IL-28) and 0.1 pg/mL (TNFα). IFN-β secretion was determined by ELISA (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions. The lower limit of IFN-β detection was 50 pg/mL.
Mixed-effects ANOVA models were used for all analyses to account for repeated measures in subjects. All responses were log-transformed for analysis except CXCL8 protein. Means and confidence intervals (CIs) of log-transformed responses were back-transformed and reported on the original scale of measurement. In the absence of significant interaction effects, the main effects for asthma status, RV versus mock treatment, and drug, are reported. Associations between viral RNA and cytokine mRNA and protein levels were tested in linear models of CXCL10 with RV, asthma, and RV × asthma interaction as covariates. Pearson correlation coefficients were calculated for relationships between RV and cytokine mRNA and protein. P values < 0.05 were considered statistically significant.
Drug effects on cytokine induction
Effects of drugs on cytokine secretion by BE cells from all subjects (mean † , 95% CI, n = 17)
3940 (3454, 4426)
3546 (2935, 4157)
4279 (3901, 4657)
3780 (3292, 4268)
7.2 (5.3, 9.8)
2.7 (1.6, 4.6)**
3.8 (2.4, 6.1)**
3.3 (1.8, 5.8)**
6.7 (3.9, 11)
0.5 (0.2, 1.4)**
2.4 (0.7, 7.8)*
0.8 (0.2, 2.6)**
4163 (3540, 4786)
3504 (2993, 4015)**
4116 (3675, 4557)
3634 (3164, 4104)*
44 (7.0, 249)
29 (9.8, 84)
34 (11, 112)
34 (12, 99)
8.1 (4.7, 14)
1.5 (0.5, 4.6)**
2.7 (0.8, 9.2)**
1.3 (0.4, 4.4)**
2.2 (1.4, 3.2)
2.0 (1.2, 3.8)
2.8 (1.8, 4.3)
2.6 (1.7, 4.0)
32 (14, 74)
19 (8.9, 42)
24 (9.4, 61)
17 (6.7, 42)
In RV-infected samples, treatment with both drugs alone and the combination inhibited secretion of TNFα, but effects on CXCL10 were not significant (Table 3). Treatment with either budesonide or the combination of budesonide and formoterol also inhibited CXCL8 secretion after RV infection. There were no significant treatment effects of budesonide, formoterol, or the combination on RV-induced IFNB1 or IL28 mRNAs (p = 0.27 and 0.14 respectively, Table 3).
Asthma effects on cytokine induction
Effects of asthma status on baseline (Mock) and RV-induced cytokine secretion and interferon mRNA expression (geometric mean, 95% CI)
P value (asthma vs. normal)
3.4 (2.2, 5.1)
5.3 (3.5, 8.2)
50.6 (21.6, 118.1)**
10.1 (4.2, 24.4)
3.2 (1.3, 7.9)
1.4 (0.6, 3.5)
4.7 (1.9, 11.3)*
1.4 (0,6, 3.5)
IFNB1 mRNA (fold increase)
3.4 (2.2, 5.3)
1.5 (1.0, 2.5)
IL28 mRNA (fold increase)
49 (21, 112)
9.2 (3.8, 22)
To determine whether this difference was related to measurement of protein vs. mRNA, we also compared RV RNA to induction of CXCL10 mRNA. RV RNA was positively correlated with CXCL10 mRNA in both groups (no asthma r = 0.61, p < 0.0001; asthma r = 0.57, p < 0.0001), but the slope was significantly greater in the no asthma group (interaction term p = 0.0008; Figure 4D). In this case, a 10-fold increase in RV RNA was associated with a 56-fold increase (95% CI 14, 230) in CXCL10 mRNA in the normal group, compared to a 2.8-fold increase (95% CI 1.2, 6.8) in CXCL10 mRNA in the asthma group.
In addition to serving as host cells for viral infections, airway epithelial cells are important initiators of the local antiviral immune response through the production of chemokines, pro-inflammatory cytokines, and interferons . These studies were conducted to determine the effects of budesonide, formoterol and the combination of these two drugs on RV-induced cytokine responses of cultured primary BE cells. Because primary cells obtained by bronchoscopy are usually low in number and have limited growth potential, we have used high but still clinically relevant concentration of drugs that had shown strong inhibitory effects in vitro[24–26] instead of testing multiple drug concentrations. The results show that RV infection stimulated CXCL10 secretion and an antiviral response as indicated by induction of IFNB1 and IL28 mRNA. Secretion of CXCL10 was significantly inhibited by budesonide, formoterol and their combination in mock-infected cells, but none of the treatments significantly affected RV-16-induced CXCL10 secretion. Notably, budesonide, formoterol or their combination did not significantly affect antiviral responses as measured by IFNB1 and IL28 mRNA expression, nor did they significantly affect viral replication. In contrast, treatment with these drugs inhibited TNFα secretion with or without infection, and budesonide or combination of budesonide and formoterol inhibited CXCL8 secretion after infection. When considered together, these findings suggest that budesonide and formoterol hinder BE cell secretion of specific pro-inflammatory cytokines without affecting epithelial antiviral responses.
Another goal of these studies was to test for allergic asthma effects on epithelial cell immune responses to RV. In cells from normal individuals, RV infection induced secretion of CXCL10 and TNFα; these responses were significantly blunted in the asthma group. Furthermore, although levels of interferon protein secretion were undetectable in most cultures, viral infection consistently upregulated IFNB1 and IL28 mRNA, and these responses were also lower in the asthma group. Some [27, 28], but not all [17, 18, 29], previous studies have suggested that epithelial cell interferon responses are lower in BE cells from subjects with asthma, and these low interferon responses were inversely related to viral replication. In contrast, interferon responses were positively related to viral replication in our study, and the lower interferon responses observed in the asthma group were associated with a trend for lower viral replication. Although the reason for lower replication in the asthma group is unclear, we speculate that use of inhaled or oral corticosteroids by the subjects with asthma could have suppressed ICAM-1 expression, and that this suppression may have persisted through several cell generations in tissue culture. Interestingly, post hoc analyses suggest that allergic asthma status also affected the relationship between viral replication and induction of CXCL10, a chemokine for Th1 cells and mononuclear cells that is strongly induced by RV infection both in vitro and in vivo[10, 17, 30]. In our study, the positive correlation between RV RNA and CXCL10 mRNA and protein was blunted in subjects with allergic asthma (Figure 4C and 4D). Additional studies are warranted to determine whether this effect is also present in vivo.
Budesonide and formoterol combination therapy has been used extensively in the treatment of asthma, and controls daily symptoms as well as reducing the risk for asthma exacerbations . The fact that most asthma exacerbations in children and about half of those in adults have viral infection as a contributing factor  suggests that combination therapy may specifically inhibit virus-induced exacerbations. Our findings as well as some previously published in vitro results [24, 25] suggest that budesonide and formoterol-mediated reduction of BE cell inflammatory responses may contribute to this effect. Skevaki et al.  have shown that the combination of budesonide and formoterol administered post infection suppressed RV-induced pro-inflammatory cytokines (CCL5, CXCL8 and CXCL10) and the remodeling-associated growth factor (VEGF) in BE cells in a synergistic or additive manner. In our experimental model, pre-treatment with budesonide alone or in combination with formoterol also inhibited pro-inflammatory cytokines (CXCL10, TNFα and CXCL8) at baseline and/or after RV infection. Synergistic or additive suppression of RV-induced chemokines was also observed after pre-treatment of cultured BE cells with fluticasone propionate and salmeterol . In RV-stimulated peripheral blood mononuclear cells, the combination of budesonide and formoterol inhibited CXCL10 and also antiviral responses (IFN-α secretion and expression of the type I interferon inducible genes) . In contrast, we did not observe significant inhibitory effects of the drugs on RV-induced upregulation of CXCL10 secretion or type I and III interferon genes (e.g. IFNB1 and IL28) in primary BE cells. Interestingly, RV-16 infection has been shown to reduce glucocorticoid sensitivity of airway epithelial cells in vitro through activation of c-Jun N-terminal kinase (JNK) and nuclear factor κB (NF-κB) signaling pathways; however, potential effects of combined treatment with beta agonists have not been studied .
This study has several strengths, and some limitations, that should be considered in interpreting these results. Strengths of the study include the use of primary cultures of BE cells, as well as including cells from donors who were characterized for allergy and asthma. The donors had moderate or severe allergic asthma with well-characterized abnormalities in lung function. Furthermore, we used highly purified preparations of RV-16, as well as sensitive and specific viral diagnostics (real-time RT-PCR) for these studies. One limitation of the study is that the asthma and no asthma groups also differed in allergic status, and so some of the differences attributed to asthma could be mediated by allergy. In addition, each of the subjects with asthma was treated with inhaled or oral corticosteroids, and these medications were continued up until the time of bronchoscopy for reasons of safety. Although effects of the corticosteroid preparations may have been partially washed out under the conditions in tissue culture, pre-study treatment could have influenced findings in vitro. These in vitro studies enabled us to isolate responses of BE cells to virus and drugs, however, it is obvious that epithelial cell responses to viral infection as well as viral clearance are affected by other cells and cytokines in the airway milieu [33, 34], and correlation with clinical findings is needed to establish relevance in vivo. It has been shown that the proportion of RV-16-infectible airway epithelial cells is relatively low (less 10%) in both monolayer cultures and bronchial tissue samples infected ex vivo primarily due to a low basal expression of ICAM-1, the receptor for RV-16 and other major group viruses . Therefore, the modest magnitude of virus-specific cytokine induction in these experiments was likely due to restriction of infection to the small subset of BE cells that express ICAM-1.
In summary, these findings indicate that budesonide and formoterol can inhibit inflammatory responses in vitro in primary BE cells without affecting viral replication or interfering with induction of the antiviral genes IFNB1 and IL28. These effects could potentially contribute to beneficial effects of budesonide and budesonide/formoterol combination therapy in preventing RV-induced asthma exacerbations in vivo. Furthermore, our results raise additional questions about the etiology and potential clinical significance of asthma-related differences in BE cell responses to RV infection.
The authors thank Ms Susan Costello for her technical assistance. This work was supported by investigator-initiated research grant from AstraZeneca.
- Friedlander SL, Busse WW: The role of rhinovirus in asthma exacerbations. J Allergy Clin Immunol. 2005, 116: 267-273. 10.1016/j.jaci.2005.06.003.PubMedView ArticleGoogle Scholar
- Johnston SL, Pattemore PK, Sanderson G, Smith S, Lampe F, Josephs L, et al: Community study of role of viral infections in exacerbations of asthma in 9–11 year old children. BMJ. 1995, 310: 1225-1229. 10.1136/bmj.310.6989.1225.PubMedPubMed CentralView ArticleGoogle Scholar
- Wells KE, Peterson EL, Ahmedani BK, Severson RK, Gleason-Comstock J, Williams LK: The relationship between combination inhaled corticosteroid and long-acting beta-agonist use and severe asthma exacerbations in a diverse population. J Allergy Clin Immunol. 2012, 129: 1274-1279. 10.1016/j.jaci.2011.12.974.PubMedPubMed CentralView ArticleGoogle Scholar
- Chung KF, Caramori G, Adcock IM: Inhaled corticosteroids as combination therapy with beta-adrenergic agonists in airways disease: present and future. Eur J Clin Pharmacol. 2009, 65: 853-871. 10.1007/s00228-009-0682-z.PubMedView ArticleGoogle Scholar
- Bateman ED, Harrison TW, Quirce S, Reddel HK, Buhl R, Humbert M, et al: Overall asthma control achieved with budesonide/formoterol maintenance and reliever therapy for patients on different treatment steps. Respir Res. 2011, 12: 38-10.1186/1465-9921-12-38.PubMedPubMed CentralView ArticleGoogle Scholar
- Reddel HK, Jenkins C, Quirce S, Sears MR, Bateman ED, O'Byrne PM, et al: Effect of different asthma treatments on risk of cold-related exacerbations. Eur Respir J. 2011, 38: 584-593. 10.1183/09031936.00186510.PubMedView ArticleGoogle Scholar
- Silvestri M, Serpero L, Petecchia L, Sabatini F, Cerasoli F, Rossi GA: Cytokine-activated bronchial epithelial cell pro-inflammatory functions are effectively downregulated in vitro by ciclesonide. Pulm Pharmacol Ther. 2006, 19: 210-217. 10.1016/j.pupt.2005.05.006.PubMedView ArticleGoogle Scholar
- Sabatini F, Silvestri M, Sale R, Serpero L, Raynal ME, Di BP, et al: Modulation of the constitutive or cytokine-induced bronchial epithelial cell functions in vitro by fluticasone propionate. Immunol Lett. 2003, 89: 215-224. 10.1016/S0165-2478(03)00142-1.PubMedView ArticleGoogle Scholar
- Grunberg K, Sharon RF, Hiltermann TJ, Brahim JJ, Dick EC, Sterk PJ, et al: Experimental rhinovirus 16 infection increases intercellular adhesion molecule-1 expression in bronchial epithelium of asthmatics regardless of inhaled steroid treatment. Clin Exp Allergy. 2000, 30: 1015-1023. 10.1046/j.1365-2222.2000.00854.x.PubMedView ArticleGoogle Scholar
- Spurrell JC, Wiehler S, Zaheer RS, Sanders SP, Proud D: Human airway epithelial cells produce IP-10 (CXCL10) in vitro and in vivo upon rhinovirus infection. Am J Physiol Lung Cell Mol Physiol. 2005, 289: L85-L95. 10.1152/ajplung.00397.2004.PubMedView ArticleGoogle Scholar
- Schroth MK, Grimm E, Frindt P, Galagan DM, Konno SI, Love R, et al: Rhinovirus replication causes RANTES production in primary bronchial epithelial cells. Am J Respir Cell Mol Biol. 1999, 20: 1220-1228. 10.1165/ajrcmb.20.6.3261.PubMedView ArticleGoogle Scholar
- Kirchberger S, Majdic O, Stockl J: Modulation of the immune system by human rhinoviruses. Int Arch Allergy Immunol. 2007, 142: 1-10. 10.1159/000095993.PubMedView ArticleGoogle Scholar
- Kallal LE, Lukacs NW: The role of chemokines in virus-associated asthma exacerbations. Curr Allergy Asthma Rep. 2008, 8: 443-450. 10.1007/s11882-008-0084-9.PubMedView ArticleGoogle Scholar
- Edwards MR, Johnson MW, Johnston SL: Combination therapy: synergistic suppression of virus-induced chemokines in airway epithelial cells. Am J Respir Cell Mol Biol. 2006, 34: 616-624. 10.1165/rcmb.2005-0385OC.PubMedView ArticleGoogle Scholar
- Davies JM, Carroll ML, Li H, Poh AM, Kirkegard D, Towers M, et al: Budesonide and formoterol reduce early innate anti-viral immune responses in vitro. PLoS One. 2011, 6: e27898-10.1371/journal.pone.0027898.PubMedPubMed CentralView ArticleGoogle Scholar
- Gustafson LM, Proud D, Hendley JO, Hayden FG, Gwaltney JM: Oral prednisone therapy in experimental rhinovirus infections. J Allergy Clin Immunol. 1996, 97: 1009-1014. 10.1016/S0091-6749(96)80077-7.PubMedView ArticleGoogle Scholar
- DeMore JP, Weisshaar EH, Vrtis RF, Swenson CA, Evans MD, Morin A, et al: Similar colds in subjects with allergic asthma and nonatopic subjects after inoculation with rhinovirus-16. J Allergy Clin Immunol. 2009, 124: 245-52. 10.1016/j.jaci.2009.05.030. 252PubMedPubMed CentralView ArticleGoogle Scholar
- Bochkov YA, Hanson KM, Keles S, Brockman-Schneider RA, Jarjour NN, Gern JE: Rhinovirus-induced modulation of gene expression in bronchial epithelial cells from subjects with asthma. Mucosal Immunol. 2010, 3: 69-80. 10.1038/mi.2009.109.PubMedPubMed CentralView ArticleGoogle Scholar
- Lee WM, Wang WS, Rueckert RR: Complete sequence of the RNA genome of human rhinovirus 16, a clinically useful common cold virus belonging to the ICAM-1 receptor group. Virus Genes. 1995, 9: 177-181. 10.1007/BF01702661.PubMedView ArticleGoogle Scholar
- Mosser AG, Vrtis R, Burchell L, Lee WM, Dick CR, Weisshaar E, et al: Quantitative and qualitative analysis of rhinovirus infection in bronchial tissues. Am J Respir Crit Care Med. 2005, 171: 645-651. 10.1164/rccm.200407-970OC.PubMedView ArticleGoogle Scholar
- Sherry B, Rueckert R: Evidence for at least two dominant neutralization antigens on human rhinovirus 14. J Virol. 1985, 53: 137-143.PubMedPubMed CentralGoogle Scholar
- He JQ, Sandford AJ, Wang IM, Stepaniants S, Knight DA, Kicic A, et al: Selection of housekeeping genes for real-time PCR in atopic human bronchial epithelial cells. Eur Respir J. 2008, 32: 755-762. 10.1183/09031936.00129107.PubMedView ArticleGoogle Scholar
- Holgate ST, Roberts G, Arshad HS, Howarth PH, Davies DE: The role of the airway epithelium and its interaction with environmental factors in asthma pathogenesis. Proc Am Thorac Soc. 2009, 6: 655-659. 10.1513/pats.200907-072DP.PubMedView ArticleGoogle Scholar
- Korn SH, Jerre A, Brattsand R: Effects of formoterol and budesonide on GM-CSF and IL-8 secretion by triggered human bronchial epithelial cells. Eur Respir J. 2001, 17: 1070-1077. 10.1183/09031936.01.00073301.PubMedView ArticleGoogle Scholar
- Skevaki CL, Christodoulou I, Spyridaki IS, Tiniakou I, Georgiou V, Xepapadaki P, et al: Budesonide and formoterol inhibit inflammatory mediator production by bronchial epithelial cells infected with rhinovirus. Clin Exp Allergy. 2009, 39: 1700-1710. 10.1111/j.1365-2222.2009.03307.x.PubMedView ArticleGoogle Scholar
- Strandberg K, Palmberg L, Larsson K: Effect of budesonide and formoterol on IL-6 and IL-8 release from primary bronchial epithelial cells. J Asthma. 2008, 45: 201-203. 10.1080/02770900801890372.PubMedView ArticleGoogle Scholar
- Wark PA, Johnston SL, Bucchieri F, Powell R, Puddicombe S, Laza-Stanca V, et al: Asthmatic bronchial epithelial cells have a deficient innate immune response to infection with rhinovirus. J Exp Med. 2005, 201: 937-947. 10.1084/jem.20041901.PubMedPubMed CentralView ArticleGoogle Scholar
- Contoli M, Message SD, Laza-Stanca V, Edwards MR, Wark PA, Bartlett NW, et al: Role of deficient type III interferon-lambda production in asthma exacerbations. Nat Med. 2006, 12: 1023-1026. 10.1038/nm1462.PubMedView ArticleGoogle Scholar
- Lopez-Souza N, Favoreto S, Wong H, Ward T, Yagi S, Schnurr D, et al: In vitro susceptibility to rhinovirus infection is greater for bronchial than for nasal airway epithelial cells in human subjects. J Allergy Clin Immunol. 2009, 123: 1384-1390. 10.1016/j.jaci.2009.03.010.PubMedPubMed CentralView ArticleGoogle Scholar
- Wark PA, Bucchieri F, Johnston SL, Gibson PG, Hamilton L, Mimica J, et al: IFN-gamma-induced protein 10 is a novel biomarker of rhinovirus-induced asthma exacerbations. J Allergy Clin Immunol. 2007, 120: 586-593. 10.1016/j.jaci.2007.04.046.PubMedView ArticleGoogle Scholar
- Gern JE: The ABCs of rhinoviruses, wheezing, and asthma. J Virol. 2010, 84: 7418-7426. 10.1128/JVI.02290-09.PubMedPubMed CentralView ArticleGoogle Scholar
- Papi A, Contoli M, Adcock IM, Bellettato C, Padovani A, Casolari P, et al: Rhinovirus infection causes steroid resistance in airway epithelium through nuclear factor kappaB and c-Jun N-terminal kinase activation. J Allergy Clin Immunol. 2013, (in press) http://dx.doi.org/10.1016/j.jaci.2013.05.028Google Scholar
- Korpi-Steiner NL, Valkenaar SM, Bates ME, Evans MD, Gern JE, Bertics PJ: Human monocytic cells direct the robust release of CXCL10 by bronchial epithelial cells during rhinovirus infection. Clin Exp Allergy. 2010, 40: 1203-1213. 10.1111/j.1365-2222.2010.03546.x.PubMedPubMed CentralView ArticleGoogle Scholar
- Kato A, Favoreto S, Avila PC, Schleimer RP: TLR3- and Th2 cytokine-dependent production of thymic stromal lymphopoietin in human airway epithelial cells. J Immunol. 2007, 179: 1080-1087.PubMedPubMed CentralView ArticleGoogle Scholar
- Mosser AG, Brockman-Schneider RA, Amineva SP, Burchell L, Sedgwick JB, Busse WW, et al: Similar frequency of rhinovirus-infectable cells in upper and lower airway epithelium. J Infect Dis. 2002, 185: 734-743. 10.1086/339339.PubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.