Table 2 Real-time PCR primers used for mRNA quantification
From: Budesonide and formoterol effects on rhinovirus replication and epithelial cell cytokine responses
Target gene | Sequence( 5’-3’) | Primer length | Amplicon size (bp) |
|---|---|---|---|
PPIA-f† | TCATCTGCACTGCCAAGACTG | 21 | |
PPIA-r† | CATGCCTTCTTTCACTTTGCC | 21 | 71 |
IFNB1-f | AGGACGCCGCATTGACC | 17 | |
IFNB1-r | ATTCCAGCCAGTGCTAGATGA | 21 | 83 |
IL28-f | GCTGACCGTGACTGGAGCA | 19 | |
IL28-r | GACAGGGACTTGAACTGGGCTA | 22 | 93 |
TNF-f | TCGGCCCGACTATCTCGAC | 19 | |
TNF-r | GCGTTTGGGAAGGTTGGAT | 19 | 94 |
CXCL10-f | AAGCTCTACTGAGGTGCTATGTT | 23 | |
CXCL10-r | TGGGAAAGGTGAGGGAAATA | 20 | 72 |
CXCL8-f | CAGTTTTGCCAAGGAGTGCTAA | 22 | |
CXCL8-r | GGTGGAAAGGTTTGGAGTATGTC | 23 | 69 |