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Influence of β2 adrenergic receptor genotype on risk of nocturnal ventilation in patients with Duchenne muscular dystrophy

Respiratory Research volume 20, Article number: 221 (2019) Cite this article

Abstract

Duchenne muscular dystrophy (DMD) is a progressive neuromuscular disease resulting in severe respiratory derangements. As such, DMD patients are at a high risk of nocturnal hypoventilation, thereby requiring nocturnal ventilation (NV). To this end, NV is an important clinical milestone in the management of DMD. Emerging evidence suggests that ß2 adrenergic receptors (ADRB2) may play a role in determining respiratory function, whereby more functional ADRB2 genotype variants (e.g., Gly16) are associated with improved pulmonary function and respiratory muscle strength. These findings suggest that the more functional ADRB2 genotype may help to preserve respiratory function in patients with DMD. The purpose of this study was to identify the influence of ADRB2 genotype on the risk of NV use in DMD. Data from the CINRG Duchenne Natural History Study including 175 DMD patients (3–25 yrs) were analyzed focusing on ADRB2 genotype variants. Time-to-event analyses were used to examine differences in the age at prescription of full-time NV use between genotypes. There were no differences between genotype groups in age, height, weight, corticosteroid use, proportion of ambulatory patients, or age at loss of ambulation. DMD patients expressing the Gly16 polymorphism had a significantly (P < 0.05) lower mean age at NV prescription compared with those patients expressing the Arg16 polymorphism (21.80 ± 0.59 yrs. vs 25.91 ± 1.31 yrs., respectively). In addition, a covariate-adjusted Cox model revealed that the Gly16 variant group possessed a 6.52-fold higher risk of full-time NV use at any given age compared with the Arg16 polymorphism group. These data suggest that genetic variations in the ADRB2 gene may influence the age at which DMD patients are first prescribed NV, whereby patients with the Gly16 polymorphism are more likely to require NV assistance at an earlier age than their Arg16 counterparts.

Background

It is estimated that 32% of Duchenne muscular dystrophy (DMD) patients suffer from nighttime alveolar hypoventilation as a result of sleep-disordered breathing [1]. Sleep-disordered breathing in this population is a result of respiratory weakness, and is associated with cardiac morbidity, neurocognitive deficits, and impaired lung function [1, 2]. As respiratory muscle weakness progresses, it results in the inability to generate a sufficient respiratory pressure to maintain adequate alveolar ventilation, leading to constant alveolar hypoventilation [3]. In patients with DMD, sleep-disordered breathing tends to precede daytime hypoventilation, which is a significant contributor to hospitalization and mortality rates [4]. The age at first use of nocturnal ventilation (NV) is therefore considered an important clinical milestone in these patients, and indicates significant respiratory derangement [1, 5]. It is therefore important to understand the factors which affect the risk of nocturnal hypoventilation, such that we may identify novel therapeutic interventions to delay dependence on NV, and offset the progression to day-time ventilator assistance, ultimately decreasing hospitalization and mortality rates, and improving quality-of-life.

Respiratory muscle weakness in DMD is characterized by a progressive loss in the ability to generate respiratory pressures, resulting in severe ventilatory derangements [6, 7]. Decreases in respiratory pressure and airflow generation can be attributed to a primary weakness of the diaphragm secondary to intramuscular remodeling in DMD [6]. This remodeling is characterized by an increased resting diaphragm thickness due to an infiltration and deposition of non-contractile elements (pseudo-hypertrophy), concomitant with the loss of sarcomeres in series [8, 9]. These dystrophic alterations together adversely affect the length-tension relationship and contractility of the diaphragm [8]. Due to the progressive nature of respiratory weakness in DMD, it is imperative that therapeutic targets are identified to slow respiratory muscle degradation, and thus decrease the risk of NV prescription.

One novel pathway that has been identified as capable of attenuating skeletal muscle degradation in DMD is the β2-adrenergic receptor (ADRB2) coupled pathway [10,11,12]. β2-adrenergic receptors influence bulk muscle size, strength and regeneration [13, 14]. ADRB2 stimulation has also been shown to: (i) increase diaphragmatic cross-sectional area, strength, and contractility [11, 12, 15]; (ii) improve mucocilliary clearance [16,17,18]; (iii) inhibit inflammatory pathways [19,20,21,22,23]; and to (iv) inhibit calpain activity and decrease its concentration [24,25,26,27,28]. In light of the above, the functionality of ADRB2 may play a significant role in the protection of respiratory muscles from dystrophic pathways, and provide a novel therapeutic target in DMD to delay the dependence on NV.

The single nucleotide polymorphism (SNP) rs1042713, situated within the coding sequence of ADRB2, causes the substitution of an Arginine (Arg) with a glycine (Gly) at amino acid position 16 of the receptor, improving its functionality. In fact, the Gly16 polymorphism is globally expressed, has a higher receptor density, and is more resistant to receptor down-regulation than its Arg16 counterpart [29, 30]. The Gly16 polymorphism is associated with sustained bronchodilation at rest and following intense exercise, and improved lung function in healthy pediatric patients and patients with heart failure [31,32,33]. The association between the Gly16 polymorphism and improved lung function in healthy and diseased populations suggests a possible therapeutic target for preserving respiratory function and delaying NV dependence in DMD patients. However, to date, no studies have investigated the influence of ADRB2 genotype on respiratory insufficiency in this clinical population.

The aim of this study was to examine the influence of ADRB2 genotype on the risk of full-time NV prescription in patients with DMD. Given that ADRB2 stimulation may provide several benefits to respiratory function (e.g., increased diaphragmatic contractility, improved mucocilliary clearance, inhibited inflammatory pathways and calpain activity), we hypothesized that DMD patients with the more “functional” ADRB2 polymorphism (i.e., Gly16) would demonstrate a lower risk of NV use compared with DMD patients expressing the less functional polymorphism (i.e., Arg16).

Methods

Participants

Data analyzed for this study were a part of a larger dataset from the Cooperative International Neuromuscular Research Group Duchenne Natural History Study (CINRG-DNHS). All participants included in this study and/or their legal guardians consented specifically to genotyping of genetic variants for research purposes, and the study was approved by local institutional or ethics review boards at each participating institution. One-hundred seventy-five patients with a clinical diagnosis of DMD (ages 3–25 years at entry into the study) were identified and included in the dataset used in this study. Patients were followed for a maximum of 9.7 years. This study focused on a functional ADRB2 protein-altering variant at codon 16 employing an Exome Chip (a genotyping chip focused on variants situated within gene-coding portions of the genome). Exome Chip genotyping and data cleaning methods in the CINRG-DNHS cohorts have been previously described [34].

Data analyses

Patient age, height, weight, corticosteroid use, ambulatory status, and forced vital capacity (FVC) at entry into the study were obtained from the CINRG-DNHS database. All reported patient heights were calculated from ulnar length, as described by others [35]. Corticosteroid use was derived from clinically reported start and stop dates. Nocturnal ventilation status was determined by the clinic visit at which full-time NV (e.g., Bi-PAP mask, n = 40; Bi-PAP nasal pillows, n = 9; C-PAP, n = 3; and mouthpiece, n = 3) was first prescribed to the patient.

Statistical analyses

Group demographics were compared using a one-way analysis of variance (ANOVA). To investigate differences among the specific genotype groups, a Tukey honest significant difference (HSD) post-hoc comparison was used. Genotype differences in the risk of NV use at any given age were estimated by a Kaplan-Meier analysis and the log-rank test. A Cox proportional hazard (PH) model was used to examine the effects of genotype variant on the risk of NV in DMD patients, after adjusting for patient age, weight, ambulatory status, corticosteroid use, and FVC. Time dependence of the covariates was ruled out after inspection of the Schoenfeld residuals scores [36]. We chose to group ADRB2 genotypes according to a dominant model for the Gly allele, i.e. homozygous for Arg at amino acid spot 16 (henceforth named “Arg16 group”, n = 26) vs. homozygous or heterozygous for Gly at amino acid 16 (“Gly16 group”, n = 149) because preliminary analyses of the data demonstrated phenotypic similarity of heterozygotes with Gly homozygotes. Furthermore, this dominant model is consistent with previous literature [32, 33, 36]. For all covariates entered into the Kaplan-Meier and Cox PH analyses, outliers were identified as observations above or below the 95th percentile for residual deviance score. Additional observations were removed from the analysis if they did not have an FVC within 6 months of study entry or were already on NV at study entry (i.e. left-censored). All statistical comparisons were made using a statistical software packages (RStudio; RStudio Inc., Boston, MA, USA, version 1.1.456). Statistical analyses were considered significant if P < 0.05.

Results

Subject characteristics

Genotypes at rs1042713 were as follows: AA (Arg/Arg) n = 26, AG (Arg/Gly) n = 90, and GG (Gly/Gly) n = 59. This genotype distribution does not violate Hardy-Weinberg equilibrium, and the observed minor allele frequency of 0.41 is close to that reported in global populations (0.42 reported in ExAC). There were no differences in age, height, weight, corticosteroid use, number of ambulatory patients, or age of loss of ambulation between genotype groups (Table 1). Furthermore, there was no difference in age at loss of ambulation between Gly16 and Arg16 genotype groups (10.96 ± 0.23 yrs. vs 11.93 ± 0.75 yrs., respectively). A total of 78 patients were non-ambulatory at entry into the study, consisting of 65 patients in the Gly16 group, and 13 patients in the Arg16 group (72 and 50% of the genotype sample populations, respectively, χ2 p = 0.058).

Table 1 Subject characteristics
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Kaplan-Meier analysis

Two observations were identified as outliers in a preliminary time-to-event analysis of age at NV dependence, and were omitted from further analyses (173 observations remaining). The results of the Kaplan-Meier analysis are illustrated in Fig. 1. The mean and median age at first use of NV are presented in Table 2. DMD participants with the Gly16 polymorphism demonstrated a higher risk (P < 0.05) of use of NV at any given age compared with those expressing the Arg16 polymorphism (21.80 ± 0.59 yrs. vs 25.91 ± 1.31 yrs., respectively).

Fig. 1
figure 1

The cumulative risk of NV in DMD patients stratified by ADRB2 genotype. The cumulative risk of nocturnal ventilation in DMD patients was calculated from the survival curve produced by the Kaplan-Meier analysis

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Table 2 Kaplan-Meier mean and median ages at first use of NV in patients with DMD
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Cox proportional Hazard

Thirty-one observations were omitted from further analyses (144 observations remaining). These observations were identified as outliers (n = 9), as not having an FVC measure (n = 7), or being on full-time NV use at entry into the study (i.e. left-censored, n = 15) in a preliminary analysis of the Cox PH model. The results of the Cox PH analysis are presented in Table 3. Patient age at study entry, ambulatory status, and FVC were strong, negative predictors of the risk of NV assistance in patients with DMD at any given age (P < 0.05). In contrast, ADRB2 genotype was identified as a strong, positive predictor of NV use in DMD patients (P < 0.05). Specifically, those DMD participants with the Gly16 genotype variant were approximately 6.52 times more likely to be given NV assistance at any given age than those with the Arg16 polymorphism. Patient weight was also identified as a positive predictor of full-time NV use (P < 0.05). The cumulative risk of NV use in DMD patients stratified by ADRB2 genotype are presented in Fig. 2.

Table 3 Cox regression analysis of the NV risk at any given age in patients with DMD
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Fig. 2
figure 2

The cumulative risk of NV in DMD patients stratified by ADRB2 genotype. The cumulative risk function was obtained from Cox regression modeling of the risk of nocturnal ventilation where genotype group, patient age at entry into the study, ambulatory status, corticosteroid-use, weight, and FVC were entered into the model as covariates. The risk curves was produced for each genotype variant by holding all other covariates in the Cox model constant at their respective means (Age = 12.2 yrs.; Mass = 43.1 kg; Corticosteroid-use = 3.4 yrs.; Ambulatory status = 0.60; FVC = 1.71)

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Discussion

The original hypothesis of this study was that DMD patients with the Gly16 polymorphism would have a reduced risk of using NV at any given age compared with those patients expressing the Arg16 polymorphism. The rationale for this hypothesis lay on the basis that ADBR2 stimulation may: increase diaphragmatic cross-sectional area, strength, and contractility [11, 12, 15]; improve mucocilliary clearance [16,17,18]; inhibit inflammatory pathways [19,20,21,22,23]; and inhibit calpain activity and decrease concentration [24,25,26,27,28]. As such, it was expected that possessing the “functional” ADBR2 genotype (i.e., Gly16) would confer a positive effect on retarding the progressive loss of respiratory function and, by extension, delaying the age at dependence on NV in DMD patients. Our results did not support this hypothesis. Instead, the present study demonstrated that DMD patients with the Gly16 polymorphism display an approximate 6.5-fold per-year increase in the risk of becoming dependent on NV than those with the Arg16 variant. We propose two potential explanations for these unexpected findings: that the Gly16 polymorphism may (i) increase the rate of contraction-induced injuries and (ii) may increase intracellular [Ca2+].

ADRB2 stimulation increases myofibril expression of type IIa myosin heavy chain isoform in animal models, promoting a fiber-type shift from slow-oxidative to more fast-glycolytic muscle fibers [37, 38]. Type II fibers have an increased peak contractile strength compared with type I fibers [39]. Indeed, healthy adults expressing the Gly16 polymorphism often display increased muscular strength and power relative to their Arg16 counterparts [36]. While it may seem at first that increased strength is beneficial for respiratory muscle fatigue and weakness, the augmentation of contractile force likely increases the number, and rate of contraction-induced injury in DMD patients. Dystrophin-deficient muscle fibers lack adequate membrane stability to efficiently distribute forces associated with myofibril contraction across the sarcolemma [40]. As a result, dystrophin-deficient muscle fibers are highly susceptible to contraction-induced injuries — a significant component of muscle fiber degradation in DMD [41]. Further, the type I to type II fiber type shift renders the muscle fiber more susceptible to eccentric contraction-induced damage, and increased fatigability [42, 43]. In light of the above, it is at least conceivable that the Gly16 polymorphism, by increasing the rate of contraction-induced injuries, hastens the progression of respiratory muscle weakness in DMD patients, leading to a higher risk of NV use.

A secondary explanation for our current findings is that DMD patients with the Gly16 polymorphism, by virtue of increased ADRB2 activity [29, 30], may have incurred additional skeletal muscle damage due to relatively higher intracellular concentrations of Ca2+ ([Ca2+]). In murine models, ADRB2 stimulation enhances Ca2+ efflux from the sarcoplasmic reticulum via a G-protein linked pathway [44], promoting a rise in intracellular [Ca2+]. This facilitatory effect of ADRB2 stimulation on rising intracellular [Ca2+] is amplified by the abnormally low gene expression of regucalcin in DMD patients, i.e., the principal Ca2+-binding protein in the diaphragm [45]. Further to the above, the potentially higher rate of contraction-induced injuries in DMD patients with the Gly16 polymorphism (see above) may promote further Ca2+ influx into the cytosol from the extracellular space [46]. It is emphasized that increased intracellular [Ca2+] promotes calpain activity, upregulating proteolytic cellular damage and myofibril degradation [44, 45]. As such, we speculate that the Gly16 polymorphism is associated with poorer intracellular [Ca2+] homeostasis and greater calpain activity, the result of which may hasten the onset of respiratory muscle weakness, leading to a higher risk of NV use than those patients expressing the Arg16 polymorphism.

Implications of our findings

Pilot studies have shown an improvement in isometric knee-extensor strength and manual muscle test scores in DMD patients following 12 weeks of ADRB2 agonist treatment [47]. A larger follow-up study also demonstrated that ADRB2 agonist treatment improves lean body mass and time to walk/run 30 ft. in patients with DMD [48]. Importantly, however, both study populations consisted of young DMD patients whose ages ranged between 5 to 11 years. We emphasize here that although our findings demonstrate that the Gly16 polymorphism confers a higher risk of NV use at any given age, the absolute difference in absolute risk between Gly16 and Arg16 groups was marginal for preadolescent and adolescent patients (Fig. 3). Indeed, the absolute risk difference between genotype groups was less than 1% up to 22 years of age in our cohort of DMD patients. The difference in risk of NV between groups widened thereafter to reach > 25% at patient ages of 27 years and older. One may infer from these observations that the increase in risk of NV use in the Gly16 compared with the Arg16 polymorphism is relatively trivial below the adolescent years, and becomes more important when DMD patients enter the second decade of life. Further studies are needed to examine whether the effects of the Gly16 polymorphism on respiratory outcomes in DMD are indeed dependent on patient age.

Fig. 3
figure 3

The absolute difference in risk of NV in DMD patients stratified by ADRB2 genotype. The risk curve is representative of the absolute difference in the cumulative risk for each genotype. The cumulative risks for each genotype was obtained from the Cox regression modeling of the risk of nocturnal ventilation where genotype group, patient age at entry into the study, ambulatory status, corticosteroid-use, weight, and FVC were entered into the model as covariates. The reference lines denote the absolute difference in risk between genotype variants at the ages of 22 and 27 years of age. Note that the difference in risk of NV between Arg16 and Gly16 genotype groups is marginal (< 1%) at ages below 22, yet widens greatly to over 25% by the age of 27 years

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Other factors influencing risk of nocturnal ventilation in DMD

Our Cox regression analysis demonstrated that patient age at entry into the study, ambulatory status, weight, and FVC were also significant predictors of the risk of NV in DMD patients. Firstly, it is expected that FVC should confer a protective (i.e., negative) effect on the risk of NV use in DMD as FVC decline is strongly correlated with the onset of nocturnal hypoventilation and the need for NV assistance in patients with DMD [49]. In fact, FVC is the primary clinical measure of respiratory function in this population and is often used as a metric for the need for NV prescription [49]. Secondly, it is not surprising that ambulatory status decreased the risk of NV in our cohort, seeing that DMD patients who are ambulatory typically display a better clinical prognosis [50]. Thirdly, patient weight was also identified as a significant, negative risk factor of NV – an expected finding given that weight is negatively associated with respiratory function and ambulatory status in this population [49]. And finally, patient age at entry into the study was also a negative predicator of the risk of NV use. This may be due to a number of patients who were not prescribed NV during the study follow-up period were also older at entry into the study. One explanation may be these patients having entered into the study at a later age as a result of being healthier and living longer without severe respiratory weakness or derangement.

Methodological considerations

It is emphasized that data for this study was collected prior to the publication of the 2018 DMD Care Considerations and the DNHS protocol did not specify when clinicians had to implement NV (e.g. at a certain FVC, pCO2, or nocturnal desaturation event threshold). Therefore, the clinically adopted thresholds may vary between clinics [49]. However, recommendations for the monitoring of sleep quality, symptoms of sleep-disordered breathing, and annual polysomnography with continuous CO2 to detect nighttime hypoventilation and the need for NV precede the current DMD Care Considerations [51]. As such, it may be assumed patients were properly monitored and that clinical standards of care were similar between populations.

Conclusions

The findings of the present study indicate that DMD patients with the Gly16 ADRB2 polymorphism are approximately 6.5 times more likely to require full-time NV assistance at a given age compared with those patients expressing the Arg16 polymorphism. Despite prior speculation that ADRB2 stimulation may be a useful therapeutic approach, our data suggest caution in using ADRB2 agonist treatment for post-adolescent DMD patients at risk for nocturnal hypoventilation.

Availability of data and materials

The data that support the findings of this study are available from CINRG but restrictions apply to the availability of these data, which were used under license for the current study, and so are not publicly available. Data are however available from the authors upon reasonable request and with permission of CINRG.

Abbreviations

ADRB2:

Beta-2 adrenergic receptor

ANOVA:

Analysis of variance

Arg:

Arginine

Ca2+ :

Calcium

CINRG-DNHS:

Cooperative International Neuromuscular Research Group Duchenne Natural History Study

DMD:

Duchenne muscular dystrophy

FVC:

Forced vital capacity

Gly:

Glycine

HSD:

Honest significant difference

NV:

Nocturnal ventilation

PH:

Proportional hazard

References

  1. Hoque R. Sleep-disordered breathing in Duchenne muscular dystrophy: an assessment of the literature. J Clin Sleep Med. 2016;12(6):905–11.

    Article  PubMed  PubMed Central  Google Scholar 

  2. Schechter MS. Technical Report: Diagnosis and Management of Childhood Obstructive Sleep Apnea Syndrome. Pediatrics. 2002;109(4):69e.

    Article  Google Scholar 

  3. Toussaint M, Steens M, Soudon P. Lung function accurately predicts Hypercapnia in patients with Duchenne muscular dystrophy. Chest. 2007;131(2):368–75.

    Article  PubMed  Google Scholar 

  4. Bach JR, Ishikawa Y, Kim H. Prevention of pulmonary morbidity for patients with Duchenne muscular dystrophy. Chest. 1997;112(4):1024–8.

    Article  CAS  PubMed  Google Scholar 

  5. Wollinsky KH, Kutter B, Geiger PM. Long-term ventilation of patients with Duchenne muscular dystrophy: experiences at the neuromuscular Centre Ulm. Acta Myol. 2012;31(3):170–8.

    PubMed  PubMed Central  Google Scholar 

  6. De Bruin PF, Ueki J, Bush A, Khan Y, Watson A, Pride NB. Diaphragm thickness and inspiratory strength in patients with Duchenne muscular dystrophy. Thorax. 1997;52(5):472–5.

    Article  PubMed  PubMed Central  Google Scholar 

  7. Bianchi C, Baiardi P. Cough peak flows: standard values for children and adolescents. Am J Phys Med Rehabil. 2008;87(6):461–7.

    Article  PubMed  Google Scholar 

  8. Beck J, Weinberg J, Hamnegård C-H, Spahija J, Olofson J, Grimby G, et al. Diaphragmatic function in advanced Duchenne muscular dystrophy. Neuromuscul Disord. 2006;16(3):161–7.

    Article  PubMed  Google Scholar 

  9. Khirani S, Ramirez A, Aubertin G, Boulé M, Chemouny C, Forin V, et al. Respiratory muscle decline in duchenne muscular dystrophy. Pediatr Pulmonol. 2014;49(5):473–81.

    Article  PubMed  Google Scholar 

  10. Church JE, Trieu J, Sheorey R, Chee AYM, Naim T, Baum DM, et al. Functional β-Adrenoceptors are important for early muscle regeneration in mice through effects on myoblast proliferation and differentiation. PLoS One. 2014;9(7):e101379.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  11. Silva MT, Wensing LA, Brum PC, Câmara NO, Miyabara EH. Impaired structural and functional regeneration of skeletal muscles fromβ2-adrenoceptor knockout mice. Acta Physiol. 2014;211(4):617–33.

    Article  CAS  Google Scholar 

  12. Smith WN, Dirks A, Sugiura T, Muller S, Scarpace P, Powers SK. Alteration of contractile force and mass in the senescent diaphragm with β2-agonist treatment. J Appl Physiol. 2002;92(3):941–8.

    Article  CAS  PubMed  Google Scholar 

  13. Connuck DM, Sleeper LA, Colan SD, Cox GF, Towbin JA, Lowe AM, et al. Characteristics and outcomes of cardiomyopathy in children with Duchenne or Becker muscular dystrophy: a comparative study from the pediatric cardiomyopathy registry. Am Heart J. 2008;155(6):998–1005.

    Article  PubMed  PubMed Central  Google Scholar 

  14. Kaspar RW, Allen HD, Montanaro F. Current understanding and management of dilated cardiomyopathy in Duchenne and Becker muscular dystrophy. J Am Acad Nurse Pract. 2009;21(5):241–9.

    Article  PubMed  PubMed Central  Google Scholar 

  15. Pellegrino MA, D'Antona G, Bortolotto S, Boschi F, Pastoris O, Bottinelli R, et al. Clenbuterol antagonizes glucocorticoid-induced atrophy and fibre type transformation in mice. Exp Physiol. 2003;89(1):89–100.

    Article  CAS  Google Scholar 

  16. Sanderson MJ, Dirksen ER. Mechanosensitive and Beta-adrenergic control of the Ciliary beat frequency of mammalian respiratory tract cells in culture. Am Rev Respir Dis. 1989;139(2):432–40.

    Article  CAS  PubMed  Google Scholar 

  17. Bennett WD. Effect of β-adrenergic agonists on mucociliary clearance. J Allergy Clin Immunol. 2002;110(6):S291–S7.

    Article  CAS  PubMed  Google Scholar 

  18. Verdugo P, Johnson NT, Tam PY. Beta-adrenergic stimulation of respiratory ciliary activity. J Appl Physiol. 1980;48(5):868–71.

    Article  CAS  PubMed  Google Scholar 

  19. Farmer P, Pugin J. β-Adrenergic agonists exert their “anti-inflammatory” effects in monocytic cells through the IκB/NF-κB pathway. Am J Phys Lung Cell Mol Phys. 2000;279(4):L675–L82.

    CAS  Google Scholar 

  20. Ye RD. β-Adrenergic agonists regulate NF-κB activation through multiple mechanisms. Am J Phys Lung Cell Mol Phys. 2000;279(4):L615–L7.

    CAS  Google Scholar 

  21. Talmadge J, Scott R, Castelli P, Newman-Tarr T, Lee J. Molecular pharmacology of the beta-adrenergic receptor on THP-1 cells. Int J Immunopharmacol. 1993;15(2):219–28.

    Article  CAS  PubMed  Google Scholar 

  22. De Paepe B, Creus KK, Martin J-J, De Bleecker JL. Upregulation of chemokines and their receptors in duchenne muscular dystrophy: potential for attenuation of myofiber necrosis. Muscle Nerve. 2012;46(6):914–6.

    Article  CAS  Google Scholar 

  23. Barros Maranhão J, de Oliveira MD, Maurício AF, de Carvalho SC, Ferretti R, Pereira JA, et al. Changes in calsequestrin, TNF-α, TGF-β and MyoD levels during the progression of skeletal muscle dystrophy inmdxmice: a comparative analysis of the quadriceps, diaphragm and intrinsic laryngeal muscles. Int J Exp Pathol. 2015;96(5):285–93.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  24. Navegantes LCC, Resano NMZ, Migliorini RH, Kettelhut ÍC. Role of adrenoceptors and cAMP on the catecholamine-induced inhibition of proteolysis in rat skeletal muscle. Am J Physiol Endocrinol Metab. 2000;279(3):E663–E8.

    Article  CAS  PubMed  Google Scholar 

  25. Navegantes LCC, Machado CR, Resano NMZ, Migliorini RH, Kettelhut IC. ß2-agonists and cAMP inhibit protein degradation in isolated chick (Gallus domesticus) skeletal muscle. Br Poult Sci. 2003;44(1):149–54.

    Article  CAS  PubMed  Google Scholar 

  26. Shiraha H, Glading A, Chou J, Jia Z, Wells A. Activation of m-Calpain (Calpain II) by epidermal growth factor is limited by protein kinase a phosphorylation of m-Calpain. Mol Cell Biol. 2002;22(8):2716–27.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Reiken S, Lacampagne A, Zhou H, Kherani A, Lehnart SE, Ward C, et al. PKA phosphorylation activates the calcium release channel (ryanodine receptor) in skeletal muscle. J Cell Biol. 2003;160(6):919–28.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Parr T, Sensky PL, Arnold MK, Bardsley RG, Buttery PJ. Effects of epinephrine infusion on expression of Calpastatin in porcine cardiac and skeletal muscle. Arch Biochem Biophys. 2000;374(2):299–305.

    Article  CAS  PubMed  Google Scholar 

  29. Tang W. The Arg16Gly polymorphism of the β2-adrenergic receptor and left ventricular systolic function. Am J Hypertens. 2003;16(11):945–51.

    Article  CAS  PubMed  Google Scholar 

  30. Daly CJ, McGrath JC. Previously unsuspected widespread cellular and tissue distribution of β-adrenoceptors and its relevance to drug action. Trends Pharmacol Sci. 2011;32(4):219–26.

    Article  CAS  PubMed  Google Scholar 

  31. Groenning BA, Nilsson JC, Sondergaard L, Fritz-Hansen T, Larsson HBW, Hildebrandt PR. Antiremodeling effects on the left ventricle during beta-blockade with metoprolol in the treatment of chronic heart failure. J Am Coll Cardiol. 2000;36(7):2072–80.

    Article  CAS  PubMed  Google Scholar 

  32. Kim WJ, Oh Y-M, Sung J, Lee YK, Seo JB, Kim N, et al. CT scanning-based phenotypes vary with ADRB2 polymorphisms in chronic obstructive pulmonary disease. Respir Med. 2009;103(1):98–103.

    Article  PubMed  Google Scholar 

  33. Zhang G, Hayden CM, Khoo S-K, Laing IA, Turner S, Landau L, et al. Association of haplotypes of β2-adrenoceptor polymorphisms with lung function and airway responsiveness in a pediatric cohort. Pediatr Pulmonol. 2006;41(12):1233–41.

    Article  CAS  PubMed  Google Scholar 

  34. Bello L, Kesari A, Gordish-Dressman H, Cnaan A, Morgenroth LP, Punetha J, et al. Genetic modifiers of ambulation in the cooperative international neuromuscular research group Duchenne natural history study. Ann Neurol. 2015;77(4):684–96.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. Gauld LM, Kappers J, Carlin JB, Robertson CF. Height prediction from ulna length. Dev Med Child Neurol. 2004;46(7):475–80.

    Article  PubMed  Google Scholar 

  36. Kelley EF, Johnson BD, Snyder EM. Beta-2 adrenergic receptor genotype influences power output in healthy subjects. J Strength Cond Res. 2017;31(8):2053–9.

    Article  PubMed  PubMed Central  Google Scholar 

  37. Hagg A, Colgan TD, Thomson RE, Qian H, Lynch GS, Gregorevic P. Using AAV vectors expressing the β2-adrenoceptor or associated Gα proteins to modulate skeletal muscle mass and muscle fibre size. Sci Rep. 2016;6:23042.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Gehrig SM, Koopman R, Naim T, Tjoakarfa C, Lynch GS. Making fast-twitch dystrophic muscles bigger protects them from contraction injury and attenuates the dystrophic pathology. Am J Pathol. 2010;176(1):29–33.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  39. Kupa EJ, Roy SH, Kandarian SC, Luca CJD. Effects of muscle fiber type and size on EMG median frequency and conduction velocity. J Appl Physiol. 1995;79(1):23–32.

    Article  CAS  PubMed  Google Scholar 

  40. Prior TW. Duchenne muscular dystrophy diagnostics: it only gets better but some of the same challenges remain. Clin Chem. 2015;61(6):792–3.

    Article  CAS  PubMed  Google Scholar 

  41. Lynch GS. Role of contraction-induced injury in the mechanisms of muscle damage in muscular dystrophy. Clin Exp Pharmacol Physiol. 2004;31(8):557–61.

    Article  CAS  PubMed  Google Scholar 

  42. Vijayan K, Thompson JL, Norenberg KM, Fitts RH, Riley DA. Fiber-type susceptibility to eccentric contraction-induced damage of hindlimb-unloaded rat AL muscles. J Appl Physiol. 2001;90(3):770–6.

    Article  CAS  PubMed  Google Scholar 

  43. Ryall JG, Lynch GS. The potential and the pitfalls of beta-adrenoceptor agonists for the management of skeletal muscle wasting. Pharmacol Ther. 2008;120(3):219–32.

    Article  CAS  PubMed  Google Scholar 

  44. Kim MO, Na SI, Lee MY, Heo JS, Han HJ. Epinephrine increases DNA synthesis via ERK1/2s through cAMP, Ca2+/PKC, and PI3K/Akt signaling pathways in mouse embryonic stem cells. J Cell Biochem. 2008;104(4):1407–20.

    Article  CAS  PubMed  Google Scholar 

  45. Doran P, Dowling P, Donoghue P, Buffini M, Ohlendieck K. Reduced expression of regucalcin in young and aged mdx diaphragm indicates abnormal cytosolic calcium handling in dystrophin-deficient muscle. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 2006;1764(4):773–85.

    Article  CAS  Google Scholar 

  46. Navegantes LCC, Baviera AM, Kettelhut IC. The inhibitory role of sympathetic nervous system in the Ca2+−dependent proteolysis of skeletal muscle. Braz J Med Biol Res. 2009;42(1):21–8.

    Article  CAS  PubMed  Google Scholar 

  47. Fowler EG, Graves MC, Wetzel GT, Spencer MJ. Pilot trial of albuterol in Duchenne and Becker muscular dystrophy. Neurology. 2004;62(6):1006–8.

    Article  CAS  PubMed  Google Scholar 

  48. Skura CL, Fowler EG, Wetzel GT, Graves M, Spencer MJ. Albuterol increases lean body mass in ambulatory boys with Duchenne or Becker muscular dystrophy. Neurology. 2008;70(2):137–43.

    Article  CAS  PubMed  Google Scholar 

  49. Birnkrant DJ, Bushby K, Bann CM, Alman BA, Apkon SD, Blackwell A, et al. Diagnosis and management of Duchenne muscular dystrophy, part 2: respiratory, cardiac, bone health, and orthopaedic management. Lancet Neurol. 2018;17(4):347–61.

    Article  PubMed  PubMed Central  Google Scholar 

  50. Koeks Z, Bladen CL, Salgado D, van Zwet E, Pogoryelova O, McMacken G, et al. Clinical outcomes in Duchenne muscular dystrophy: a study of 5345 patients from the TREAT-NMD DMD global database. J Neuromuscul Dis. 2017;4(4):293–306.

    Article  PubMed  PubMed Central  Google Scholar 

  51. Bushby K, Finkel R, Birnkrant DJ, Case LE, Clemens PR, Cripe L, et al. Diagnosis and management of Duchenne muscular dystrophy, part 2: implementation of multidisciplinary care. Lancet Neurol. 2010;9(2):177–89.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The authors are sincerely grateful for the patients that dedicated their time and agreed to participate in the collection of data used in this study. This study could not have been done without their participation.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Department of Kinesiology, University of Minnesota, Minneapolis, MN, USA

    Eli F. Kelley & Eric M. Snyder

  2. Department of Cardiovascular Diseases, Mayo Clinic, RO_GE_MN_10, 1216 2nd Street SW, Rochester, MN, 55902, USA

    Eli F. Kelley & Troy J. Cross

  3. University of California Davis Medical Center, Sacramento, CA, USA

    Craig M. McDonald

  4. Binghamton University – SUNY, Binghamton, NY, USA

    Eric P. Hoffman

  5. Center for Genetic Medicine, Children’s Research Institute, Children’s National Health System, Washington, DC, USA

    Eric P. Hoffman

  6. Department of Neurosciences, University of Padova, Padova, Italy

    Luca Bello

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  1. Eli F. Kelley
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  4. Craig M. McDonald
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Consortia

CINRG Investigators

Contributions

Conceptualization, EK, TC, and LB; Methodology, EK, TC, and LB; Investigation, CINRG; Data Curation, EK, CM, EH, and LB; Writing – Original Draft Preparation, EK; Writing – Review and Editing, EK, TC, ES, CM, EH, and LB; Visualization, EK, TC, and LB. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Eli F. Kelley.

Ethics declarations

Ethics approval and consent to participate

All participants included in this study and/or their legal guardians consented specifically to genotyping of genetic variants for research purposes, and the study was approved by local institutional or ethics review boards at each participating institution.

Consent for publication

These data were approved for publication by the CINRG Publication Committee and were determined to adhere to their ethical publication guidelines and that all data were presented clearly and without falsification.

Competing interests

The authors declare that they have no competing interests.

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Kelley, E.F., Cross, T.J., Snyder, E.M. et al. Influence of β2 adrenergic receptor genotype on risk of nocturnal ventilation in patients with Duchenne muscular dystrophy. Respir Res 20, 221 (2019). https://doi.org/10.1186/s12931-019-1200-1

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  • DOI: https://doi.org/10.1186/s12931-019-1200-1

Keywords

Respiratory Research

ISSN: 1465-993X

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