- Open Access
Endogenous laminin is required for human airway smooth muscle cell maturation
© Tran et al. 2006
- Received: 20 June 2006
- Accepted: 12 September 2006
- Published: 12 September 2006
Airway smooth muscle (ASM) contraction underlies acute bronchospasm in asthma. ASM cells can switch between a synthetic-proliferative phenotype and a contractile phenotype. While the effects of extracellular matrix (ECM) components on modulation of ASM cells to a synthetic phenotype have been reported, the role of ECM components on maturation of ASM cells to a contractile phenotype in adult lung is unclear. As both changes in ECM components and accumulation of contractile ASM are features of airway wall remodelling in asthma, we examined the role of the ECM protein, laminin, in the maturation of contractile phenotype in human ASM cells.
Human ASM cells were made senescence-resistant by stable expression of human telomerase reverse transcriptase. Maturation to a contractile phenotype was induced by 7-day serum deprivation, as assessed by immunoblotting for desmin and calponin. The role of laminin on ASM maturation was investigated by comparing the effects of exogenous laminin coated on culture plates, and of soluble laminin peptide competitors. Endogenous expression of laminin chains during ASM maturation was also measured.
Myocyte binding to endogenously expressed laminin was required for ASM phenotype maturation, as laminin competing peptides (YIGSR or GRGDSP) significantly reduced desmin and calponin protein accumulation that otherwise occurs with prolonged serum deprivation. Coating of plastic cell culture dishes with different purified laminin preparations was not sufficient to further promote accumulation of desmin or calponin during 7-day serum deprivation. Expression of α2, β1 and γ1 laminin chains by ASM cells was specifically up-regulated during myocyte maturation, suggesting a key role for laminin-2 in the development of the contractile phenotype.
While earlier reports suggest exogenously applied laminin slows the spontaneous modulation of ASM to a synthetic phenotype, we show for the first time that endogenously expressed laminin is required for ASM maturation to the contractile phenotype. As endogenously expressed laminin chains α2, β1 and γ1 are uniquely increased during myocyte maturation, these laminin chains may be key in this process. Thus, human ASM maturation appears to involve regulated endogenous expression of a select set of laminin chains that are essential for accumulation of contractile phenotype myocytes.
- Airway Smooth Muscle
- Serum Deprivation
- Airway Smooth Muscle Cell
- Human Airway Smooth Muscle
Remodelling of the airway wall is a feature of chronic asthma and is characterized by a number of structural changes including, but not limited to, increased mass of contractile airway smooth muscle (ASM) , and fibrosis resulting from the accumulation of extracellular matrix proteins (ECM) [2, 3]. ASM is a key determinant of airway hyperresponsiveness and remodelling in asthma. Airway myocytes are thought to have capacity to contribute to remodelling due to their ability for graded, and reversible phenotype switching, which confers broad functional capacity [4, 5]. At one extreme airway myocytes exist in an immature phenotype that is characterised by a high tendency for proliferation, expression and secretion of ECM proteins, and synthesis of inflammatory mediators in response to a number of environmental cues [4–7]. In contrast, myocytes of a mature phenotype serve a primarily contractile function and are marked by a unique repertoire of cytoskeletal and contractile apparatus proteins; including smooth muscle myosin heavy chain, SM22, desmin and calponin [5, 7–9]. Notably, however, there is evidence that contractile smooth muscle cells are capable of expressing ECM components such as glycosaminoglycans  and collagen , suggesting that ASM cells exist in a functional phenotype that is intermediate to the fully synthetic and contractile state.
Laminins are cross-shaped heterotrimeric glycoproteins of the ECM that contain one copy each of an α-, β- and γ-chain [12, 13]. The expression of laminin is tissue dependent and varies at different times during development . In the lung, the most significant changes in the expression pattern of laminin occurs between the pseudoglandular and canalicular stage, during which differentiation of ASM cells is initiated and the structural ordering of the airway wall is established .
Using antibodies that block laminin polymerisation or receptor binding to laminin, Schuger and colleagues [16, 17] showed that lung mesenchymal cell spreading on laminin-containing ECM is required for differentiation of embryonic lung mesenchymal cells into ASM cells. Moreover, similar studies with embryonic mouse organotypic and whole lung cultures reveal laminin is an essential basement membrane component necessary for both pulmonary branching morphogenesis, and for the circumferential alignment of ASM cells around the airway epithelia. Laminin required for ASM differentiation and structural organization of the airway is synthesized, in part, by the developing myocytes themselves, as suppression of α1 laminin chain secretion using brefeldin A prevents myocyte accumulation in the vicinity of developing epithelia . Notably, the airways of asthmatics show greater immunoreactivity for the α2 and β2 laminin chains compared with the airway wall of healthy controls . Also, cultured human ASM cells obtained from asthmatic patients exhibit an altered expression profile of ECM proteins that includes increased collagen type I and decreased laminin α1 chain . Furthermore, cultured human ASM cells stimulated with asthmatic serum produce increased amounts of the γ1 chain of laminin . Collectively, these observations suggest that changes in the endogenous expression of laminin by ASM cells may occur in asthma, and this could be an essential determinant of myocyte phenotype and function during disease pathogenesis.
Hirst and colleagues  showed that primary cultured human ASM cells grown on a laminin matrix proliferated much more slowly, were less responsive to mitogens, and expressed greater abundance of contractile proteins compared with cells grown on a plastic or collagen type I matrix. This suggests that exogenous laminin prevents the spontaneous modulation of contractile ASM cells to a synthetic/proliferative phenotype in cell culture. Given the emerging evidence for ASM phenotype plasticity and its potential association with pathogenesis of features of asthma such as the accumulation of contractile ASM mass and fibrosis of the airway wall, in the current study we investigated whether laminin was required for the maturation of ASM cells to a contractile phenotype in culture. For our studies we used prolonged serum deprivation to induce contractile phenotype maturation in human ASM as we have previously described [9, 23–25]. Based on expression of desmin and calponin, which are well characterised protein markers for the mature/contractile phenotype [8, 26–29], we tested the requirement of endogenously expressed laminin for contractile myocyte maturation. By elucidating the cellular and molecular mechanisms that regulate airway myocyte differentiation and phenotype modulation we hope to better understand the role of laminin in the contribution of ASM cells to the pathogenesis of asthma.
Immortalized human airway smooth muscle cell culture
For all studies at least four senescence-resistant human airway smooth muscle (HASM) cell lines were prepared using MMLV retroviral vectors to facilitate stable integration of the human telomerase reverse transcriptase gene (hTERT) as we have previously described . The primary cultured HASM cells used to generate each cell line were prepared as we have previously described from macroscopically healthy segments of 2nd-to-4th generation main bronchus obtained after lung resection surgery from patients with a diagnosis of adenocarcinoma [30, 31]. All procedures were approved by the Human Research Ethics Board (University of Manitoba). hTERT-expressing HASM cells retain the ability to express markers of the contractile phenotype including smooth muscle myosin heavy chain, calponin, sm-α-actin, and desmin to passage 10 and higher . For all experiments, passages 16–22 cultures were used. To induce 'contractile/mature' phenotype expression in subpopulations of hTERT-HASM, confluent cultures were maintained in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with ITS (insulin 5 μg/ml; transferrin 5 μg/ml, selenium 5 ng/ml) for up to 7 days as we have described .
Human ASM cells were seeded onto 100 mm dishes and left to grow to monolayer confluence in DMEM containing 10% v/v foetal bovine serum (FBS). The cells were then trypsinised and replated onto 6-well plastic, or laminin-coated culture dishes at a seeding density of 1 × 105 cells per well in DMEM containing 0.5% v/v FBS (preliminary experiments confirmed that this seeding density was sufficient to retain confluency in re-seeded cultures). Before subsequent treatments, re-plated cultures were incubated at 37°C for ~16 hrs, to ensure full cell adherence and spreading [22, 32] independent of any influences on ASM proliferation induced by the ECM (as measured from cell counts using the haemocytometer). To induce a contractile phenotype, cells were then serum deprived for up to 7 days in DMEM/ITS, a duration previously shown by our laboratory to be sufficient to induce myocyte maturation and increased expression of the smooth muscle contractile apparatus protein; calponin, and the intermediate filament protein, desmin [9, 33, 34]. Serum-free media was replaced with fresh DMEM/ITS every second day.
To examine the role of laminin in the maturation of HASM cells, laminin-competing peptides (1 μM) were added at the time of serum deprivation and re-added every second day when fresh serum-free media was replaced. The YIGSR pentapeptide corresponds to the 929–933 sequence of the β chain of laminin  and is found to compete with laminin for binding to the laminin receptor. The YIGSR peptide (Sigma, Saint Louis, MO) was reconstituted in distilled water to a stock concentration of 10 mM and then diluted to 1 μM final concentration in serum-free DMEM for use in experiments. The GRGDSP peptide is derived from the amino acid sequence of the α chain of laminin. The RGD amino acid sequence is also found within fibronectin, where it was originally identified as the sequence motif that mediates cell attachment . The GRGDSP and GRADSP peptides (Calbiochem, La Jolla, CA) were reconstituted in 5% acetic acid to a stock concentration of 10 mM and then for use in experiments was diluted in serum-free DMEM to 1 μM final concentration as per previous reports . In pilot experiments (not shown), we determined that the highest concentration of vehicle acetic acid (0.0005%) was well below the threshold concentration for influencing HASM cell proliferation, desmin or calponin protein accumulation.
Laminin coating of culture plates
To examine the effect of exogenous laminin, plastic cell culture plates were coated with various forms of laminin according to previously described methods . For coating experiments, all laminin preparations were reconstituted in sterile PBS and then diluted to 10 μg/ml in PBS. Briefly, each laminin preparation was adsorbed to 6-well plates for 6 hrs at room temperature. Non-specific binding sites were blocked for 30 minutes with PBS containing 0.1% w/v BSA, at room temperature. Prior to seeding of cells onto the laminin-coated plates, the plates were washed with DMEM. The laminin preparations used included laminin prepared from Engelbreth-Holm-Swarm (EHS) murine sarcoma (Sigma, St. Louis, MO) that predominately consists of the laminin trimer α1, β1 and γ1. The degree of homology between full length murine and human laminin is at least 78%, with laminin receptor binding domains exhibiting 100% homology [14, 38]. Two laminin preparations from freshly frozen human placenta tissue were also used (gift from Dr J. Wilkins of the University of Manitoba, Canada). Placental laminin was isolated by affinity purification with monoclonal 4E10 or 5H2 antibodies, which recognize laminin β1 and α2 chains, respectively [39, 40].
Measurement of ASM mature-marker proteins
For analysis of myocyte phenotype, protein lysates were harvested 16 hrs after re-plating (basal/Day 0) and following 7 days serum-deprivation (Day 7). Cells were washed twice with ice-cold PBS and extracted with ice-cold lysis buffer (100 mM NaCl; 10 mM Tris-HCl, pH 7.5; 2 mM EDTA; 0.5% w/v deoxycholate; 1% v/v triton X-100; 1 mM phenylmethylsulphonylfluoride; 10 mM MgCl2; 5 μg/ml aprotinin; 100 μM sodium orthovanadate). The cells were scraped, transferred to 1.5 ml plastic tubes, centrifuged (760 × g, 5 min) and the supernatant stored at -20°C. Protein content in supernatant samples was determined using the Bio-Rad protein assay (BioRad, Hercules, CA). The samples (12–15 μg protein per lane) were separated electrophoretically under reducing conditions on an 8% SDS-polyacrylamide gel and proteins were transferred onto nitrocellulose membranes for western blotting. Membranes were blocked with 5% w/v skim milk in Tris Buffered Saline (10 mM Tris HCl, pH8, 150 mM NaCl) and 0.1% v/v Tween-20, then incubated with primary antibodies, diluted in 1% w/v skim milk, to desmin (1:500 dilution) or calponin (1:2,000 dilution). The membranes were developed by subsequent incubation with secondary horseradish-peroxidase-conjugated antibody, then visualized with enhanced chemiluminescence reagents (Amersham, Buckinghamshire, UK). Membranes were re-probed with antibodies for β-actin to normalize for equal loading of all samples. Scanning and quantification of the relative protein abundance was performed using the Epson Perfection 4180 Station and TotalLab TL100 software (Nonlinear Dynamics, Durham, NC). Protein results were expressed as fold increment over basal (Day 0) relative to β-actin.
List of primers for laminin chains used in Real-Time PCR
NCBI accession number
Laminin α1 chain
Forward 5'TGG GTG TGG GAT TTC TTA GC 3'
Reverse 5'CCT GAC CGT CTA CCC AGT GT 3'
Laminin α2 chain
Forward 5'GGC TTA TTC AGC TGG CAG AG 3'
Reverse 5'ATT CTC CCA GGG ACT TTG CT 3'
Laminin β1 chain
Forward 5'AAC GTG GTT GGA AGA ACC TG 3'
Reverse 5'ACA CTC CCT GGA AAC AGT GG 3'
Laminin β2 chain
Forward 5'CCT AGC CCT GTG AGC AAC TC 3'
Reverse 5'GTC TGT CAG GCT CAG GGT GT 3'
Laminin γ1 chain
Forward 5'AAT CCG TAT GGG ACC ATG AA 3'
Reverse 5'TCA CAC CTC TCA CAG CCT TG 3'
Forward 5'CGC CGC TAG AGG TGA AAT TC 3'
Reverse 5'TTG GCA AAT GCT TTC GCT C 3'
All chemicals used were of analytical grade or higher. All compounds [mouse anti-calponin antibody (clone hCP, C2687), YIGSR, laminin-EHS] were purchased from Sigma (St. Luois, MO) unless stated otherwise. GRGDSP and GRADSP peptides were obtained from Calbiochem (EMB Biosciences Inc. La Jolla, CA). Rabbit anti-desmin antibody (H-76, sc14026) was purchased from Santa Cruz Biotechnology Inc (Santa Cruz, CA). Antibodies to laminin chains α2, β1, β2 and γ1 were a gift from Dr. Eva Engvall (The Burnham Institute, La Jolla, CA, USA).
Data are expressed as mean and standard error of the mean (SEM) of observations obtained from ASM cells cultured from at least four different cell lines. All experiments were carried out in duplicate. Data were expressed as fold increment over basal (Day 0) relative to β-actin (protein) or 18s rRNA (mRNA) and results were analysed using one-way ANOVA, with repeated measures, followed by Bonferroni's post hoc t test, where appropriate. A probability value of P < 0.05 was considered significant.
Effect of blocking laminin binding on HASM maturation
Effect of exogenous laminin on HASM maturation
Profile of endogenous laminin chains synthesized by HASM cells
Absolute ΔCT values for laminin chain mRNA expression
Laminin chain mRNA (absolute ΔCT values)
Laminin α1 chain
22.2 ± 0.5
23.5 ± 0.9
Laminin α2 chain
10.8 ± 0.9
8.8 ± 0.4
Laminin β1 chain
9.0 ± 0.5
7.5 ± 0.7
Laminin β2 chain
9.3 ± 0.3
8.8 ± 0.9
Laminin γ1 chain
8.8 ± 0.4
7.9 ± 0.6
This study was completed to extend understanding of the role of laminin in phenotype expression of human ASM cells from the adult lung. There is ample evidence that laminin has both anti-proliferative effects and slows spontaneous phenotype modulation of airway, visceral and vascular smooth muscle cells [22, 45–47]. However, despite a number of elegant studies investigating mesenchyme differentiation in airways of embryonic mouse lung cultures , the direct role of laminin in phenotype maturation of differentiated airway myocytes from the mature lung has not been dissected. Our current studies using soluble peptide inhibitors of laminin binding sites demonstrate for the first time that laminin chains endogenously expressed by HASM cells are required for maturation to a contractile phenotype. This is supported by our observations that each of the laminin competing peptides used, YIGSR and GRGDSP, completely inhibited accumulation of contractile phenotype protein markers (desmin and calponin), in a well established serum-free cell culture system that promotes myocyte maturation [9, 23, 25]. Furthermore, we have characterized the profile of laminin chains expressed by our human airway myocyte cultures, and documented that expression of the constituents of laminin-2 (α2, β1, and γ1 chains) increases concomitantly with myocyte maturation. In addition, we show that in contrast to the potential for exogenous laminin coated onto culture dishes to prevent phenotype modulation, it is not sufficient to augment endogenous laminin-dependent maturation of cultured HASM. Collectively, these findings demonstrate an essential role for endogenous laminin in determining phenotype expression of HASM cells from the adult lung, and thus could represent a mechanism for intrinsic regulation of the contribution of HASM to changes in airway structure in health and disease.
Laminin is a trimer consisting of three polypeptide chains, α, β and γ, which possess a number of potential binding sites for receptors including the integrins [48–55] and other non-integrin subtypes [56–58]. A number of studies have used soluble peptides that compete with known sequences for laminin receptors, and these have generated considerable understanding of the role of laminins in cell biology. For our studies we use two active peptides, YIGSR and GRGDSP, and an inactive control, GRADSP to modulate myocyte interactions with the ECM. As in previous studies using other cell systems, the peptide inhibitors for laminin binding that we used were well suited for in vitro investigation, and thus provided an important tool to determine the role of endogenously expressed laminins in HASM phenotype maturation. None of the peptides induced cell death or detachment. Consistent with published studies [32, 36, 59–61] our preliminary experiments did reveal that both active peptides prevent adhesion of newly plated cells; this observation was a key element for our experimental design, in which we only added peptides in serum free media after re-plated myocytes had fully attached and spread in the presence of 0.5% FBS.
The YIGSR peptide corresponds to amino acids 929–933 of the β chain of laminin  and has been used in numerous experimental conditions in vitro and in vivo [42, 62–66]. For example, YIGSR inhibits tumor growth and tumor cell deposition in the bone, liver, and kidney in a mouse model of B-cell lymphoma . Also, YIGSR reduces the formation of lung colonies in mice injected with melanoma cells and inhibits melanoma cell migration in vitro . We used YIGSR as a laminin-specific inhibitor due to its selectively for a unique sequence in the laminin β1 chain. As laminin β1 chain is a component of a number of laminin isoforms we cannot be entirely certain which isoforms were most affected in our study, however based on our expression studies that show evidence for concomitant increase in laminin α2 chain with serum deprivation, it appears that laminin-2 was likely a principal target.
The GRGDSP peptide mimics sequence present in the α chain of laminin that shares homology with a number of other ECM proteins, such as fibronectin [36, 61]. Hayman and colleagues  showed that the threshold concentration of GRGDSP that induces normal rat kidney cell detachment from fibronectin and vitronectin occurs at concentrations above 1 μM. Though, we used a concentration of 1 μM for all peptides in the current study, the effects of GRGDSP, which were similar to that for YIGSR, cannot be exclusively attributed to the inhibition of laminin binding. Nonetheless, in light of the similarity of the cell responses to YIGSR or GRGDSP in our studies, it is likely that effects of the latter on laminin interactions is a significant element of the responses measured.
Different splice variants of the each of the α, β and γ laminin chains can combine to produce at least 15 functional laminin isoforms. Using immunohistochemical approaches, various laminin chains have been shown to be expressed in the lung and specifically in the airway around smooth muscle bundles [15, 19, 67–72]. However no studies to date have conclusively shown the presence of distinct staining for specific laminin isoforms due to lack of available antibodies that detect the presence of laminin trimers. We used three different laminin preparations to coat culture dishes and assess the capacity of exogenous laminin to promote phenotype maturation of HASM. EHS laminin is widely used and consists primarily of laminin-1 . As a broader spectrum of laminin isoforms is expressed in the lungs we also used laminin affinity purified from human placenta using β1 laminin chain-selective antibodies. Many of the known laminin isoforms possess a β1 chain, including laminin-1, 2, 6, 8, and 10 [14, 69, 73]. Laminins-2 (α2 β1γ1), 4 (α2 β2γ1), 8 (α4 β1γ1) and 10 (α5 β1γ1) are expressed in human placenta [39, 74, 75], thus there is considerable overlap with laminins expressed in the lung. To assess the effects of a more defined lung-relevant laminin we also used an anti-α2 laminin chain affinity purified preparation from human placenta that contained only laminins-2 and 4. Despite using distinct laminin preparations, we did not observe any augmentation of myocyte maturation; the accumulation of desmin and calponin was similar to control cultures. Each laminin preparation induced a web-like HASM cells pattern, which is characteristic of that induced by coating with laminin-1 [22, 44]. Collectively, although our studies with competing peptides indicate endogenous laminin is required for HASM maturation, it appears that addition of exogenous laminin is not sufficient to promote this further.
As our studies with competing peptides established a role for endogenous laminin expression in determining HASM phenotype maturation, we profiled laminin chain expression in cultured myocytes. Under serum-fed conditions, characterised by the presence of HASM cells of the proliferative/synthetic phenotype, the profile of laminin chain mRNA consisted predominately of equal abundance of β1, β2 and γ1 laminin chains, whereas α2 and α1 laminin chains were less abundant. This is not unexpected given that the β and γ laminin chains are ubiquitously expressed  and α laminin chain expression chiefly contributes to the heterogeneity seen for tissue-specific and developmental stage-specific expression of the laminin isoforms [69, 76–79].
Our studies revealed that during HASM maturation, there is a concomitant and significant increase in mRNA and protein for α2, β1 and γ1 laminin chains. These results complement our experiments using α and β1 laminin chain competing peptides that show selective endogenous laminin expression is a determinant of phenotype maturation. That we observed an increased expression of α2, β1, and γ1 laminin with phenotype maturation suggests the involvement of laminin-2 in this process. A number of studies also show that laminin-1 is prominent in the developing lung where it determines mesenchymal cell differentiation, and thereafter laminin-2 accumulates in the adult lung airways [15–17, 80, 81]. Moreover, our focus on laminin chains that comprise laminin-1 and 2 was due to a lack of available antibodies to perform a comprehensive survey of all α, β and γ laminin chains . Our studies provide new insight concerning endogenous laminin expression by adult HASM cells, and how expression of specific laminin chains, such as α2, β1, and γ1, correlates with changes in HASM phenotype. Moreover, these data suggest that the process of HASM cell maturation may be regulated intrinsically through changes in the synthesis of specific laminin chains.
A large number of studies show that dynamic changes in laminin expression is a key factor in normal lung development [16, 80, 81], and also in a number of pathologies, including asthma [19, 68, 82]). In atopic asthmatics whose epithelial integrity is compromised, increased immunoreactivity for γ2 is seen . There are also a number of studies that describe differences in laminin chain composition between allergic asthmatics and non-allergic asthmatics, including evidence for increases in both α2 and β2 laminin chains [19, 67, 68, 83]. Furthermore, Johnson and colleagues  report that primary cultured HASM cells exhibit an altered profile of ECM protein expression, including a decrease in laminin α1 chain, that may act as an intrinsic mechanism to promote myocyte proliferation. Similarly, HASM passively sensitized with serum from atopic asthmatics produce increased amounts of the γ1 chain of laminin . In our current study we observed changes in expression of laminin associated with HASM maturation in non-proliferating myocytes. We have previously shown that phenotype maturation is associated with myocyte hypertrophy . Of note, to date there are limited numbers of studies that directly demonstrate ASM cells proliferation in situ [85, 86], suggesting myocyte hypertrophy contributes significantly to increased muscle mass in airway remodelling. In light of our current study, it is thus tempting to speculate that documented accumulation of ECM around airway smooth muscle [87, 88], and changes in laminin chain expression in the airways of asthmatics could contribute to HASM hypertrophy associated with key features of airway remodelling in chronic asthma.
There are three major new findings from this study: (1) endogenously expressed laminin is both required and sufficient for the maturation of HASM to a contractile phenotype; (2) exogenous laminin (types 1, 2, 3, 4, 6, 8, and 10) are not sufficient to augment the phenotype maturation and accumulation of calponin and desmin that occurs during prolonged serum deprivation of cultured HASM cells; and, (3) HASM cells in culture express a unique profile of laminin chains, and appear to selectively increase expression of α2, β1, and γ1 chains, which comprise laminin-2, in conditions that promote maturation to a contractile phenotype. These results provide strong support for the role of laminin in the maintenance and regulation of ASM phenotype in the adult lung and thus, may be an important mechanism regulating the contribution of myocytes to airways remodelling in disease states such as asthma.
This study was supported by grants to Dr. Halayko from the Canadian Institutes of Health Research (CIHR), Manitoba Institute of Child Health, and Manitoba Health Research Council. Dr. Tran is the recipient of fellowships from GlaxoSmithKline/Canadian Lung Association/Canadian Institutes of Health Research (CIHR) and the National Training program in Allergy and Asthma-CIHR. Dr. Gerthoffer was supported by a grant from the US National Institutes of Health, HL077726. We are grateful to Dr. John Wilkins of the University of Manitoba for affinity purified human placental laminin; Dr. Eva Engvall (The Burnham Institute, La Jolla, CA, USA) for the gift of laminin chain antibodies to α2, β1, β2 and γ1. The authors wish to also thank Mr. Gerald Stelmack of the University of Manitoba, Canada for technical advice; Prof. Alastair Stewart and A/Prof. Daryl Knight for helpful discussion.
- Ebina M, Takahashi T, Chiba T, Motomiya M: Cellular hypertrophy and hyperplasia of airway smooth muscles underlying bronchial asthma. A 3-D morphometric study. Am Rev Respir Dis 1993, 148:720–726.View ArticlePubMedGoogle Scholar
- Brewster CE, Howarth PH, Djukanovic R, Wilson J, Holgate ST, Roche WR: Myofibroblasts and subepithelial fibrosis in bronchial asthma. Am J Respir Cell Mol Biol 1990, 3:507–511.View ArticlePubMedGoogle Scholar
- Jeffery PK, Wardlaw AJ, Nelson FC, Collins JV, Kay AB: Bronchial biopsies in asthma. An ultrastructural, quantitative study and correlation with hyperreactivity. Am Rev Respir Dis 1989, 140:1745–1753.View ArticlePubMedGoogle Scholar
- Halayko AJ, Amrani Y: Mechanisms of inflammation-mediated airway smooth muscle plasticity and airways remodeling in asthma. Respir Physiol Neurobiol 2003, 137:209–222.View ArticlePubMedGoogle Scholar
- Halayko AJ, Solway J: Molecular mechanisms of phenotypic plasticity in smooth muscle cells. J Appl Physiol 2001, 90:358–368.PubMedGoogle Scholar
- Howarth PH, Knox AJ, Amrani Y, Tliba O, Panettieri RAJ, Johnson M: Synthetic responses in airway smooth muscle. J Allergy Clin Immunol 2004, 114:S32–50.View ArticlePubMedGoogle Scholar
- Owens GK: Regulation of differentiation of vascular smooth muscle cells. Physiol Rev 1995, 75:487–517.PubMedGoogle Scholar
- Halayko AJ, Salari H, Ma X, Stephens NL: Markers of airway smooth muscle cell phenotype. Am J Physiol 1996, 270:L1040–51.PubMedGoogle Scholar
- Halayko AJ, Camoretti-Mercado B, Forsythe SM, Vieira JE, Mitchell RW, Wylam ME, Hershenson MB, Solway J: Divergent differentiation paths in airway smooth muscle culture: induction of functionally contractile myocytes. Am J Physiol 1999, 276:L197–206.PubMedGoogle Scholar
- Merrilees MJ, Campbell JH, Spanidis E, Campbell GR: Glycosaminoglycan synthesis by smooth muscle cells of differing phenotype and their response to endothelial cell conditioned medium. Atherosclerosis 1990, 81:245–254.View ArticlePubMedGoogle Scholar
- Ang AH, Tachas G, Campbell JH, Bateman JF, Campbell GR: Collagen synthesis by cultured rabbit aortic smooth-muscle cells. Alteration with phenotype. Biochem J 1990, 265:461–469.View ArticlePubMedPubMed CentralGoogle Scholar
- Timpl R, Rohde H, Robey PG, Rennard SI, Foidart JM, Martin GR: Laminin--a glycoprotein from basement membranes. J Biol Chem 1979, 254:9933–9937.PubMedGoogle Scholar
- Malinda KM, Kleinman HK: The laminins. Int J Biochem Cell Biol 1996, 28:957–959.View ArticlePubMedGoogle Scholar
- Engvall E: Laminin variants: why, where and when? Kidney Int 1993, 43:2–6.View ArticlePubMedGoogle Scholar
- Virtanen I, Laitinen A, Tani T, Paakko P, Laitinen LA, Burgeson RE, Lehto VP: Differential expression of laminins and their integrin receptors in developing and adult human lung. Am J Respir Cell Mol Biol 1996, 15:184–196.View ArticlePubMedGoogle Scholar
- Relan NK, Yang Y, Beqaj S, Miner JH, Schuger L: Cell elongation induces laminin alpha2 chain expression in mouse embryonic mesenchymal cells: role in visceral myogenesis. J Cell Biol 1999, 147:1341–1350.View ArticlePubMedPubMed CentralGoogle Scholar
- Schuger L: Laminins in lung development. Exp Lung Res 1997, 23:119–129.View ArticlePubMedGoogle Scholar
- Schuger L, Skubitz AP, Zhang J, Sorokin L, He L: Laminin alpha1 chain synthesis in the mouse developing lung: requirement for epithelial-mesenchymal contact and possible role in bronchial smooth muscle development. J Cell Biol 1997, 139:553–562.View ArticlePubMedPubMed CentralGoogle Scholar
- Altraja A, Laitinen A, Virtanen I, Kampe M, Simonsson BG, Karlsson SE, Hakansson L, Venge P, Sillastu H, Laitinen LA: Expression of laminins in the airways in various types of asthmatic patients: a morphometric study. Am J Respir Cell Mol Biol 1996, 15:482–488.View ArticlePubMedGoogle Scholar
- Johnson PR, Burgess JK, Underwood PA, Au W, Poniris MH, Tamm M, Ge Q, Roth M, Black JL: Extracellular matrix proteins modulate asthmatic airway smooth muscle cell proliferation via an autocrine mechanism. J Allergy Clin Immunol 2004, 113:690–696.View ArticlePubMedGoogle Scholar
- Johnson PR, Black JL, Carlin S, Ge Q, Underwood PA: The production of extracellular matrix proteins by human passively sensitized airway smooth-muscle cells in culture: the effect of beclomethasone. Am J Respir Crit Care Med 2000, 162:2145–2151.View ArticlePubMedGoogle Scholar
- Hirst SJ, Twort CH, Lee TH: Differential effects of extracellular matrix proteins on human airway smooth muscle cell proliferation and phenotype. Am J Respir Cell Mol Biol 2000, 23:335–344.View ArticlePubMedGoogle Scholar
- Mitchell RW, Halayko AJ, Kahraman S, Solway J, Wylam ME: Selective restoration of calcium coupling to muscarinic M(3) receptors in contractile cultured airway myocytes. Am J Physiol Lung Cell Mol Physiol 2000, 278:L1091–100.PubMedGoogle Scholar
- Ma X, Wang Y, Stephens NL: Serum deprivation induces a unique hypercontractile phenotype of cultured smooth muscle cells. Am J Physiol 1998, 274:C1206–14.PubMedGoogle Scholar
- Gosens R, Stelmack GL, Dueck G, McNeill KD, Yamasaki A, Gerthoffer WT, Unruh H, Soussi-Gounni A, Zaagsma J, Halayko AJ: Role of caveolin-1 in p42/p44 MAP kinase activation and proliferation of human airway smooth muscle. Am J Physiol Lung Cell Mol Physiol 2006.Google Scholar
- Frid MG, Moiseeva EP, Stenmark KR: Multiple phenotypically distinct smooth muscle cell populations exist in the adult and developing bovine pulmonary arterial media in vivo. Circ Res 1994, 75:669–681.View ArticlePubMedGoogle Scholar
- Carlsson L, Thornell LE: Desmin-related myopathies in mice and man. Acta Physiol Scand 2001, 171:341–348.View ArticlePubMedGoogle Scholar
- Paulin D, Li Z: Desmin: a major intermediate filament protein essential for the structural integrity and function of muscle. Exp Cell Res 2004, 301:1–7.View ArticlePubMedGoogle Scholar
- Frid MG, Shekhonin BV, Koteliansky VE, Glukhova MA: Phenotypic changes of human smooth muscle cells during development: late expression of heavy caldesmon and calponin. Dev Biol 1992, 153:185–193.View ArticlePubMedGoogle Scholar
- Naureckas ET, Ndukwu IM, Halayko AJ, Maxwell C, Hershenson MB, Solway J: Bronchoalveolar lavage fluid from asthmatic subjects is mitogenic for human airway smooth muscle. Am J Respir Crit Care Med 1999, 160:2062–2066.View ArticlePubMedGoogle Scholar
- Rahman MS, Yang J, Shan LY, Unruh H, Yang X, Halayko AJ, Gounni AS: IL-17R activation of human airway smooth muscle cells induces CXCL-8 production via a transcriptional-dependent mechanism. Clin Immunol 2005, 115:268–276.View ArticlePubMedGoogle Scholar
- Gloe T, Riedmayr S, Sohn HY, Pohl U: The 67-kDa laminin-binding protein is involved in shear stress-dependent endothelial nitric-oxide synthase expression. J Biol Chem 1999, 274:15996–16002.View ArticlePubMedGoogle Scholar
- Halayko AJ, Stelmack GL, Yamasaki A, McNeill K, Unruh H, Rector E: Distribution of phenotypically disparate myocyte subpopulations in airway smooth muscle. Can J Physiol Pharmacol 2005, 83:104–116.View ArticlePubMedGoogle Scholar
- Halayko AJ, Rector E, Stephens NL: Airway smooth muscle cell proliferation: characterization of subpopulations by sensitivity to heparin inhibition. Am J Physiol 1998, 274:L17–25.PubMedGoogle Scholar
- Graf J, Ogle RC, Robey FA, Sasaki M, Martin GR, Yamada Y, Kleinman HK: A pentapeptide from the laminin B1 chain mediates cell adhesion and binds the 67,000 laminin receptor. Biochemistry 1987, 26:6896–6900.View ArticlePubMedGoogle Scholar
- Pierschbacher MD, Ruoslahti E: Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule. Nature 1984, 309:30–33.View ArticlePubMedGoogle Scholar
- Hayman EG, Pierschbacher MD, Ruoslahti E: Detachment of cells from culture substrate by soluble fibronectin peptides. J Cell Biol 1985, 100:1948–1954.View ArticlePubMedGoogle Scholar
- Haaparanta T, Uitto J, Ruoslahti E, Engvall E: Molecular cloning of the cDNA encoding human laminin A chain. Matrix 1991, 11:151–160.View ArticlePubMedGoogle Scholar
- Wewer U, Albrechtsen R, Manthorpe M, Varon S, Engvall E, Ruoslahti E: Human laminin isolated in a nearly intact, biologically active form from placenta by limited proteolysis. J Biol Chem 1983, 258:12654–12660.PubMedGoogle Scholar
- Engvall E, Davis GE, Dickerson K, Ruoslahti E, Varon S, Manthorpe M: Mapping of domains in human laminin using monoclonal antibodies: localization of the neurite-promoting site. J Cell Biol 1986, 103:2457–2465.View ArticlePubMedGoogle Scholar
- Tran T, Fernandes DJ, Schuliga M, Harris T, Landells L, Stewart AG: Stimulus-dependent glucocorticoid-resistance of GM-CSF production in human cultured airway smooth muscle. Br J Pharmacol 2005, 145:123–131.View ArticlePubMedPubMed CentralGoogle Scholar
- Iwamoto Y, Robey FA, Graf J, Sasaki M, Kleinman HK, Yamada Y, Martin GR: YIGSR, a synthetic laminin pentapeptide, inhibits experimental metastasis formation. Science 1987, 238:1132–1134.View ArticlePubMedGoogle Scholar
- Hoyt DG, Rizzo M, Gerritsen ME, Pitt BR, Lazo JS: Integrin activation protects pulmonary endothelial cells from the genotoxic effects of bleomycin. Am J Physiol 1997, 273:L612–7.PubMedGoogle Scholar
- Behrens P, Meissner C, Hopfer H, Schumann J, Tan MI, Ellerbrake N, Strunck E, Vollmer G: Laminin mediates basement membrane induced differentiation of HEC 1B endometrial adenocarcinoma cells. Biochem Cell Biol 1996, 74:875–886.View ArticlePubMedGoogle Scholar
- Hayashi K, Takahashi M, Kimura K, Nishida W, Saga H, Sobue K: Changes in the balance of phosphoinositide 3-kinase/protein kinase B (Akt) and the mitogen-activated protein kinases (ERK/p38MAPK) determine a phenotype of visceral and vascular smooth muscle cells. J Cell Biol 1999, 145:727–740.View ArticlePubMedPubMed CentralGoogle Scholar
- Hayashi K, Saga H, Chimori Y, Kimura K, Yamanaka Y, Sobue K: Differentiated phenotype of smooth muscle cells depends on signaling pathways through insulin-like growth factors and phosphatidylinositol 3-kinase. J Biol Chem 1998, 273:28860–28867.View ArticlePubMedGoogle Scholar
- Freyer AM, Johnson SR, Hall IP: Effects of growth factors and extracellular matrix on survival of human airway smooth muscle cells. Am J Respir Cell Mol Biol 2001, 25:569–576.View ArticlePubMedGoogle Scholar
- Gehlsen KR, Dickerson K, Argraves WS, Engvall E, Ruoslahti E: Subunit structure of a laminin-binding integrin and localization of its binding site on laminin. J Biol Chem 1989, 264:19034–19038.PubMedGoogle Scholar
- Sonnenberg A, Linders CJ, Modderman PW, Damsky CH, Aumailley M, Timpl R: Integrin recognition of different cell-binding fragments of laminin (P1, E3, E8) and evidence that alpha 6 beta 1 but not alpha 6 beta 4 functions as a major receptor for fragment E8. J Cell Biol 1990, 110:2145–2155.View ArticlePubMedGoogle Scholar
- Sonnenberg A, Linders CJ, Daams JH, Kennel SJ: The alpha 6 beta 1 (VLA-6) and alpha 6 beta 4 protein complexes: tissue distribution and biochemical properties. J Cell Sci 1990, 96 ( Pt 2):207–217.Google Scholar
- Song WK, Wang W, Foster RF, Bielser DA, Kaufman SJ: H36-alpha 7 is a novel integrin alpha chain that is developmentally regulated during skeletal myogenesis. J Cell Biol 1992, 117:643–657.View ArticlePubMedGoogle Scholar
- Collo G, Starr L, Quaranta V: A new isoform of the laminin receptor integrin alpha 7 beta 1 is developmentally regulated in skeletal muscle. J Biol Chem 1993, 268:19019–19024.PubMedGoogle Scholar
- Song WK, Wang W, Sato H, Bielser DA, Kaufman SJ: Expression of alpha 7 integrin cytoplasmic domains during skeletal muscle development: alternate forms, conformational change, and homologies with serine/threonine kinases and tyrosine phosphatases. J Cell Sci 1993, 106 ( Pt 4):1139–1152.Google Scholar
- Ziober BL, Vu MP, Waleh N, Crawford J, Lin CS, Kramer RH: Alternative extracellular and cytoplasmic domains of the integrin alpha 7 subunit are differentially expressed during development. J Biol Chem 1993, 268:26773–26783.PubMedGoogle Scholar
- Yao CC, Breuss J, Pytela R, Kramer RH: Functional expression of the alpha 7 integrin receptor in differentiated smooth muscle cells. J Cell Sci 1997, 110 ( Pt 13):1477–1487.Google Scholar
- Ervasti JM, Campbell KP: A role for the dystrophin-glycoprotein complex as a transmembrane linker between laminin and actin. J Cell Biol 1993, 122:809–823.View ArticlePubMedGoogle Scholar
- Rao CN, Castronovo V, Schmitt MC, Wewer UM, Claysmith AP, Liotta LA, Sobel ME: Evidence for a precursor of the high-affinity metastasis-associated murine laminin receptor. Biochemistry 1989, 28:7476–7486.View ArticlePubMedGoogle Scholar
- Douville PJ, Harvey WJ, Carbonetto S: Isolation and partial characterization of high affinity laminin receptors in neural cells. J Biol Chem 1988, 263:14964–14969.PubMedGoogle Scholar
- Scaffidi AK, Petrovic N, Moodley YP, Fogel-Petrovic M, Kroeger KM, Seeber RM, Eidne KA, Thompson PJ, Knight DA: alpha(v)beta(3) Integrin interacts with the transforming growth factor beta (TGFbeta) type II receptor to potentiate the proliferative effects of TGFbeta1 in living human lung fibroblasts. J Biol Chem 2004, 279:37726–37733.View ArticlePubMedGoogle Scholar
- Scaffidi AK, Moodley YP, Weichselbaum M, Thompson PJ, Knight DA: Regulation of human lung fibroblast phenotype and function by vitronectin and vitronectin integrins. J Cell Sci 2001, 114:3507–3516.PubMedGoogle Scholar
- Pierschbacher MD, Ruoslahti E: Variants of the cell recognition site of fibronectin that retain attachment-promoting activity. Proc Natl Acad Sci U S A 1984, 81:5985–5988.View ArticlePubMedPubMed CentralGoogle Scholar
- Grant DS, Tashiro K, Segui-Real B, Yamada Y, Martin GR, Kleinman HK: Two different laminin domains mediate the differentiation of human endothelial cells into capillary-like structures in vitro. Cell 1989, 58:933–943.View ArticlePubMedGoogle Scholar
- Gloe T, Pohl U: Laminin binding conveys mechanosensing in endothelial cells. News Physiol Sci 2002, 17:166–169.PubMedGoogle Scholar
- Massia SP, Rao SS, Hubbell JA: Covalently immobilized laminin peptide Tyr-Ile-Gly-Ser-Arg (YIGSR) supports cell spreading and co-localization of the 67-kilodalton laminin receptor with alpha-actinin and vinculin. J Biol Chem 1993, 268:8053–8059.PubMedGoogle Scholar
- Sakamoto N, Iwahana M, Tanaka NG, Osada Y: Inhibition of angiogenesis and tumor growth by a synthetic laminin peptide, CDPGYIGSR-NH2. Cancer Res 1991, 51:903–906.PubMedGoogle Scholar
- Michigami T, Nomizu M, Yamada Y, Dunstan C, Williams PJ, Munday GR, Yoneda T: Growth and dissemination of a newly-established murine B-cell lymphoma cell line is inhibited by multimeric YIGSR peptide. Clin Exp Metastasis 1998, 16:645–654.View ArticlePubMedGoogle Scholar
- Amin K, Janson C, Seveus L, Miyazaki K, Virtanen I, Venge P: Uncoordinated production of Laminin-5 chains in airways epithelium of allergic asthmatics. Respir Res 2005, 6:110.View ArticlePubMedPubMed CentralGoogle Scholar
- Amin K, Ludviksdottir D, Janson C, Nettelbladt O, Bjornsson E, Roomans GM, Boman G, Seveus L, Venge P: Inflammation and structural changes in the airways of patients with atopic and nonatopic asthma. BHR Group. Am J Respir Crit Care Med 2000, 162:2295–2301.View ArticlePubMedGoogle Scholar
- Tunggal P, Smyth N, Paulsson M, Ott MC: Laminins: structure and genetic regulation. Microsc Res Tech 2000, 51:214–227.View ArticlePubMedGoogle Scholar
- Virtanen I, Gullberg D, Rissanen J, Kivilaakso E, Kiviluoto T, Laitinen LA, Lehto VP, Ekblom P: Laminin alpha1-chain shows a restricted distribution in epithelial basement membranes of fetal and adult human tissues. Exp Cell Res 2000, 257:298–309.View ArticlePubMedGoogle Scholar
- Glukhova M, Koteliansky V, Fondacci C, Marotte F, Rappaport L: Laminin variants and integrin laminin receptors in developing and adult human smooth muscle. Dev Biol 1993, 157:437–447.View ArticlePubMedGoogle Scholar
- Pierce RA, Griffin GL, Miner JH, Senior RM: Expression patterns of laminin alpha1 and alpha5 in human lung during development. Am J Respir Cell Mol Biol 2000, 23:742–747.View ArticlePubMedGoogle Scholar
- Mercurio AM: Laminin: multiple forms, multiple receptors. Curr Opin Cell Biol 1990, 2:845–849.View ArticlePubMedGoogle Scholar
- Wondimu Z, Gorfu G, Kawataki T, Smirnov S, Yurchenco P, Tryggvason K, Patarroyo M: Characterization of commercial laminin preparations from human placenta in comparison to recombinant laminins 2 (alpha2beta1gamma1), 8 (alpha4beta1gamma1), 10 (alpha5beta1gamma1). Matrix Biol 2006, 25:89–93.View ArticlePubMedGoogle Scholar
- Ehrig K, Leivo I, Argraves WS, Ruoslahti E, Engvall E: Merosin, a tissue-specific basement membrane protein, is a laminin-like protein. Proc Natl Acad Sci U S A 1990, 87:3264–3268.View ArticlePubMedPubMed CentralGoogle Scholar
- Lentz SI, Miner JH, Sanes JR, Snider WD: Distribution of the ten known laminin chains in the pathways and targets of developing sensory axons. J Comp Neurol 1997, 378:547–561.View ArticlePubMedGoogle Scholar
- Sanes JR, Engvall E, Butkowski R, Hunter DD: Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere. J Cell Biol 1990, 111:1685–1699.View ArticlePubMedGoogle Scholar
- Sorokin LM, Pausch F, Frieser M, Kroger S, Ohage E, Deutzmann R: Developmental regulation of the laminin alpha5 chain suggests a role in epithelial and endothelial cell maturation. Dev Biol 1997, 189:285–300.View ArticlePubMedGoogle Scholar
- Miner JH, Patton BL, Lentz SI, Gilbert DJ, Snider WD, Jenkins NA, Copeland NG, Sanes JR: The laminin alpha chains: expression, developmental transitions, and chromosomal locations of alpha1–5, identification of heterotrimeric laminins 8–11, and cloning of a novel alpha3 isoform. J Cell Biol 1997, 137:685–701.View ArticlePubMedPubMed CentralGoogle Scholar
- Sasaki T, Giltay R, Talts U, Timpl R, Talts JF: Expression and distribution of laminin alpha1 and alpha2 chains in embryonic and adult mouse tissues: an immunochemical approach. Exp Cell Res 2002, 275:185–199.View ArticlePubMedGoogle Scholar
- Yang Y, Palmer KC, Relan N, Diglio C, Schuger L: Role of laminin polymerization at the epithelial mesenchymal interface in bronchial myogenesis. Development 1998, 125:2621–2629.PubMedGoogle Scholar
- Christie PE, Jonas M, Tsai CH, Chi EY, Henderson WRJ: Increase in laminin expression in allergic airway remodelling and decrease by dexamethasone. Eur Respir J 2004, 24:107–115.View ArticlePubMedGoogle Scholar
- Amin K, Janson C, Boman G, Venge P: The extracellular deposition of mast cell products is increased in hypertrophic airways smooth muscles in allergic asthma but not in nonallergic asthma. Allergy 2005, 60:1241–1247.View ArticlePubMedGoogle Scholar
- Halayko AJ, Kartha S, Stelmack GL, McConville J, Tam J, Camoretti-Mercado B, Forsythe SM, Hershenson MB, Solway J: Phophatidylinositol-3 kinase/mammalian target of rapamycin/p70S6K regulates contractile protein accumulation in airway myocyte differentiation. Am J Respir Cell Mol Biol 2004, 31:266–275.View ArticlePubMedGoogle Scholar
- Benayoun L, Druilhe A, Dombret MC, Aubier M, Pretolani M: Airway structural alterations selectively associated with severe asthma. Am J Respir Crit Care Med 2003, 167:1360–1368.View ArticlePubMedGoogle Scholar
- Stewart AG: Emigration and immigration of mesenchymal cells: a multicultural airway wall. Eur Respir J 2004, 24:515–517.View ArticlePubMedGoogle Scholar
- Thomson RJ, Bramley AM, Schellenberg RR: Airway muscle stereology: implications for increased shortening in asthma. Am J Respir Crit Care Med 1996, 154:749–757.View ArticlePubMedGoogle Scholar
- Bramley AM, Roberts CR, Schellenberg RR: Collagenase increases shortening of human bronchial smooth muscle in vitro. Am J Respir Crit Care Med 1995, 152:1513–1517.View ArticlePubMedGoogle Scholar
- Gutala RV, Reddy PH: The use of real-time PCR analysis in a gene expression study of Alzheimer's disease post-mortem brains. J Neurosci Methods 2004, 132:101–107.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.