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Table 2 Absolute ΔCT values for laminin chain mRNA expression

From: Endogenous laminin is required for human airway smooth muscle cell maturation

Gene product Laminin chain mRNA (absolute ΔCT values)
  Day 0 Day 7
Laminin α1 chain 22.2 ± 0.5 23.5 ± 0.9
Laminin α2 chain 10.8 ± 0.9 8.8 ± 0.4
Laminin β1 chain 9.0 ± 0.5 7.5 ± 0.7
Laminin β2 chain 9.3 ± 0.3 8.8 ± 0.9
Laminin γ1 chain 8.8 ± 0.4 7.9 ± 0.6
  1. CT = threshold cycle/amplification cycle number. ΔCT = difference between CT value for laminin chain gene of interest versus matched 18s rRNA CT value. The smaller the ΔCT value the greater the mRNA expression. These absolute ΔCT values were used to calculate fold differences in mRNA levels presented in Figure 5 using the comparative CT method.