Atrial fibrosis in a chronic murine model of obstructive sleep apnea: mechanisms and prevention by mesenchymal stem cells
- Pablo Ramos1,
- Cira Rubies1,
- Marta Torres2,
- Montserrat Batlle1,
- Ramon Farre3,
- Josep Brugada1,
- Josep M Montserrat2,
- Isaac Almendros†2 and
- Lluís Mont†1Email author
© Ramos et al.; licensee BioMed Central Ltd. 2014
Received: 16 October 2013
Accepted: 18 March 2014
Published: 28 April 2014
OSA increases atrial fibrillation (AF) risk and is associated with poor AF treatment outcomes. However, a causal association is not firmly established and the mechanisms involved are poorly understood. The aims of this work were to determine whether chronic obstructive sleep apnea (OSA) induces an atrial pro-arrhythmogenic substrate and to explore whether mesenchymal stem cells (MSC) are able to prevent it in a rat model of OSA.
A custom-made setup was used to mimic recurrent OSA-like airway obstructions in rats. OSA-rats (n = 16) were subjected to 15-second obstructions, 60 apneas/hour, 6 hours/day during 21 consecutive days. Sham rats (n = 14) were placed in the setup but no obstructions were applied. In a second series of rats, MSC were administered to OSA-rats and saline to Sham-rats. Myocardial collagen deposit was evaluated in Picrosirius-red stained samples. mRNA expression of genes involved in collagen turnover, inflammation and oxidative stress were quantified by real time PCR. MMP-2 protein levels were quantified by Western Blot.
A 43% greater interstitial collagen fraction was observed in the atria, but not in the ventricles, of OSA-rats compared to Sham-rats (Sham 8.32 ± 0.46% vs OSA 11.90 ± 0.59%, P < 0.01). Angiotensin-I Converting Enzyme (ACE) and Interleukin 6 (IL-6) expression were significantly increased in both atria, while Matrix Metalloproteinase-2 (MMP-2) expression was decreased. MSC administration blunted OSA-induced atrial fibrosis (Sham + Saline 8.39 ± 0.56% vs OSA + MSC 9.57 ± 0.31%, P = 0.11), as well as changes in MMP-2 and IL-6 expression. Interleukin 1-β (IL-1β) plasma concentration correlated to atrial but not ventricular fibrosis. Notably, a 2.5-fold increase in IL-1β plasma levels was observed in the OSA group, which was prevented in rats receiving MSC.
OSA induces selective atrial fibrosis in a chronic murine model, which can be mediated in part by the systemic and local inflammation and by decreased collagen-degradation. MSCs transplantation prevents atrial fibrosis, suggesting that these stem cells could counterbalance inflammation in OSA.
Patients with obstructive sleep apnea (OSA) show both a high prevalence  and incidence  of atrial fibrillation (AF). In addition, OSA has been associated with a greater risk of AF recurrence after cardioversion  and catheter ablation [4, 5] and a worse response to antiarrhythmic drugs . Despite the clear association between OSA and AF, it is not firmly established whether this association is causal or mediated by other comorbidities often present in OSA-patients, such as obesity or hypertension .
Atrial structural remodeling, particularly fibrosis, is a main component in the substrate predisposing to AF . Atrial fibrosis predicts disease progression and treatment outcomes . It is known from murine models that exposure to recurrent airway obstructions promotes early myocardial inflammation leading to myocardial apoptosis at mid-term . However, it remains unknown whether chronic exposure to recurrent apneas can reach to develop atrial fibrosis, thus explaining the higher prevalence and incidence of AF observed in OSA patients. In addition, cell-based therapies emerge as an attractive alternative to classic pharmacological treatments for the prevention of such remodeling, thereby reducing AF occurrence and progression. Among the options available for cell therapy, bone marrow mesenchymal stem cells (MSC) appear as a promising source of stem cells because of their multi-lineage potential, anti-inflammatory effects [11, 12], ability to escape detection by the host immune system, and a relative ease of expansion in culture [13, 14]. Recent studies have shown that MSCs attenuate cardiac fibrosis in a variety of experimental settings [15–17]. Although the knowledge on the therapeutic role of MSC in OSA models is very limited , there is evidence that stem cells possess anti-inflammatory properties that mitigate the early inflammatory response .
The aim of our study was 1) to describe OSA-induced atrial remodeling in a chronic murine model, 2) to analyze the putative mechanisms involved and 3) to investigate whether MSC have the potential to prevent such remodeling in the same OSA model.
Experimental sleep apnea model
This study conformed to European Community (Directive 86/609/EEC) and Spanish guidelines for the use of experimental animals and was approved by the Animal Research Ethics Committee of the University of Barcelona.
The first part of the study was carried out in 30 Sprague–Dawley male rats (250–300 g and 8 weeks old at the beginning of the experiment) randomized into 2 groups: OSA and Sham. OSA-rats (n = 16) were subjected to 15-second obstructions at a rate of 60 per hour, 6 hours per day during 21 consecutive days. Sham-rats (n = 14) were placed in the setup during the same period of time (21 days, 6 hours per day), but no obstructions were applied. Rats in both groups were progressively adapted to the experimental setting by increasing the time within the OSA/Sham device at a rate of 1 hour each day, up to 6 h at the end of the first week. Rats were housed in a controlled environment (12/12-hour light/dark cycle) and fed rodent chow and tap water ad libitum.
Mesenchymal stem cells infusion
To evaluate the effect of MSC in OSA-induced structural remodeling, 8 rats undergoing the previously described OSA protocol were infused with MSC (OSA + MSC group). Seven Sham rats receiving saline vehicle (Sham + S group) were used as controls in this experiment.
MSC were obtained by culturing well-characterized Lewis rat marrow stromal cells kindly provided by the Tulane Center for Gene Therapy (New Orleans, LA, USA). The cells were cultured in MEM-alpha medium with glutamine and without ribonucleosides or deoxyribonucleosides (GIBCO, Gaithersburg, MD, USA), supplemented with 20% fetal bovine serum (HyClone Cell Culture, Cultek, Madrid, Spain), 1% antibiotic–antimycotic (containing 10,000 U/mL Penicillin G sodium; 10,000 μg/mL Streptomycin sulphate; 25 μg/mL Amphotericin B as Fungizone [GIBCO, Gaithersburg, MD, USA]) and 2% l-glutamine (GIBCO, Gaithersburg, MD, USA). The cells were grown at 37°C, 5% CO2, 100% humidity. Subconfluent cells were dissociated with 0.25% trypsin and 1 mM Ethylene Diamine Tetraacetic Acid (EDTA) in Hanks’ Balanced Salt Solution (GIBCO, Gaithersburg, MD, USA) and subcultured at low density in new culture flasks. To prepare the injection, MSC were trypsinized and 5 × 106 cells were suspended in 500 μL of Phosphate Buffered Saline (GIBCO, Gaithersburg, MD, USA). This cell preparation was slowly delivered (30 seconds) to the rat through the penile vein the first day of apneas application and every 4 days thereafter. Rats were anaesthetized with short-acting inhaled isoflourane (2%) before and during every injection.
All rats in the four experimental groups (Sham, Sham + S, OSA, OSA + MSC) were carefully inspected daily to monitor animal well-being. No rats showed any signs of stress or MSC-related side effects and thus, no rats needed to be excluded from the analysis.
Sample collection and RNA isolation
Once anesthetized with intraperitoneal urethane 10% (1 g/kg), animals were sacrificed by exsanguination through carotid artery cannulation. Blood samples were collected in EDTA tubes and centrifuged for 15 minutes at 3000 rpm. Plasma was collected and frozen at -80°C.
Hearts were quickly removed and weighted, and tissue samples were obtained from the left ventricle (LV) free wall, right ventricle (RV) free wall, and both atrial appendages: right atrium (RA) and left atrium (LA). Samples were snap-frozen in liquid nitrogen for posterior molecular biology analysis. For histological studies, transversal sections from the midventricular and basal (including both atria) regions of the heart were obtained, fixed in formol 3% for 24 hours and embedded in paraffin.
Total RNA was isolated from the 4 cardiac chambers. Myocardial tissue was first homogenized with Trizol® reagent (Life Technologies) and further purified with chloroform (C-2432, Sigma-Aldrich). Final RNA was obtained with silica columns (RNA Aqueous kit, Ambion) according to the manufacturer's protocol. The integrity of the resulting RNA was assessed in formaldehyde-denaturing agarose gels. A reverse transcription protocol with random primers was applied to 1 μg of total RNA for cDNA synthesis with the addition of RNAse inhibitors (High capacity cDNA RT kit, Life Technologies, CA, USA) with a MJ Research PTC 200 thermal cycler (MJ Research, MA, USA).
Real time polymerase chain reaction (real time PCR)
TaqMan one-gene expression assays
Angiotensin-I Converting Enzyme
Transforming Growth Factor-β1
Tissue Inhibitor of Metallopeptidase-1
Tissue Inhibitor of Metallopeptidase-2
Nitric Oxide Synthase III
Protein isolation and analysis by western blot
Myocardial proteins were extracted from each heart cavity in all experimental groups. Samples were submerged in 0.5 mL of ice-cold protein lysis buffer containing: RIPA buffer (R0278, Sigma), 1 mM phenylmethanesulfonyl fluoride (P7626, Sigma), 1 mM sodium orthovanadate (S6508, Sigma), 1 mM Pefabloc (11429868001, Roche) and complete Mini Protease Inhibitor Cocktail (11836153001, Roche). Samples were homogenized with an Omni TH homogenizer (Omni International Inc.). After 1 hour of rotation at 4°C, samples were centrifuged at 10.000 g at 4°C for 30 minutes. The upper phase was collected and the total protein concentration was quantified with the Pierce BCA protein Assay method (23227, Thermo Scientific, Pierce) relative to a BSA standard curve. Forty μg of total protein extract were loaded with a reducing buffer (2.5% of β-mercaptoethanol) to NuPage® 4–12% Bis-Tris Gel (NP0322). A western blot was performed with the Novex® gels methodology (Invitrogen). Proteins were transferred to a nitrocellulose membrane using a blot gel transfer (IB3010-01) and the iBlot® Dry Blotting System. Proper transfer was checked by Ponceau staining. After 1 hour blockade of the membrane with phosphate buffered saline solution (PBS, Fisher Scientific), 0.1% Tween 20 (P1379, Sigma-Aldrich) and 5% of skimmed milk, it was incubated overnight at 4°C with the MMP-2 primary antibody (ab37150, Abcam) diluted 1/500. Afterwards, the membrane was incubated during 1 hour with an HRP-Goat anti rabbit secondary antibody diluted 1/1000 (31460 Thermo Scientific). Final detection of the MMP-2 protein bands was accomplished with the ECL kit Supersignal West Pico Chemioluminescent Substrate (34080, Thermo Scientific).
Bands around 60 KDa for MMP-2 (in antibody and ECL incubated membranes) and around 40 KDa (in Ponceau-stained membranes) were quantified (ImageJ, NIH, Maryland, USA). Loading of each sample was normalized with the Ponceau band around 40 KDa. Results are given in arbitrary units (A.U.) as the ratio between the normalized densities of each sample divided by the normalized density of a standard loaded in each gel.
Systemic inflammation-related cytokines -proinflammatory and profibrotic Interleukin-1 beta (IL-1β), and anti-inflammatory Interleukin-10 (IL-10)- were measured in plasma obtained at sacrifice with Quantikine ELISA assays RLB00 and R1000, respectively (R&D Systems, MN, USA).
A CD90 immunofluorescence assay was performed in myocardial parafine-embedded sections from rats injected with MSC, and in MSC cultured in 30-40% confluent chamber slides (positive control). Cultured MSC were fixed with 4% paraformaldehyde. Antigenic retrieval was achieved with a sodium citrate 10 mM bath at 80°C during 40 min. After blockade of unspecific unions, samples were incubated overnight with Anti-Rat CD90, FITC conjugated antibody (MR5001, Caltag, Invitrogen) diluted 1/200. Sudan Black was used to mask auto-fluorescence. Both myocardial sections and chambers were added a DAPI-containing mounting media and covered. The same staining protocol excluding Anti-CD90 antibody addition was used as a negative control for both groups.
Myocardial fibrosis quantification
Mid-ventricular and atrial sections of paraffin-embedded tissue, 4 microns thick, were stained with Picrosirius-red as previously described . Four random pictures (40×) from RV and LV free wall, and 6 random pictures (100×) from the atria were taken from each sample with an Olympus BX51 microscope and an Olympus DP50 camera (Olympus Corporation, Japan).
To estimate hypertrophy of the cardiac chambers, RV free wall, interventricular septum (IVS) and LV free wall thickness were measured in transversal sections at mid-ventricular level. The interstitial collagen fraction was assessed with a semiautomatic color-threshold detection software (AnalySIS®, Soft Imaging System, Germany). Right and left atrial collagen fraction were quantified together. Epicardial, endocardial, and perivascular fibrosis were excluded from the analysis. All measures were carried out blind by a single investigator.
All variables followed a normal distribution (Shapiro-Wilks test) and are expressed as mean ± standard error of the mean (SEM). Grubbs test was used to exclude extreme outliers (maximum one per group). Comparisons between 2 groups were carried out with a non-paired Student’s t-test. A Pearson product–moment correlation coefficient was computed to assess the relationship between the plasmatic IL-1β levels and the myocardial collagen fraction. Statistical calculations were performed with SPSS v16.0 (SPSS Institute Inc, Cary, NC, USA). A p-value <0.05 was considered for significance.
Obstructive sleep apnea induces atrial fibrosis
The 3-week OSA protocol did not induce global or specific-chamber hypertrophy in rats. No significant differences between both groups were found in heart weight (Sham 1.21 ± 0.02 g. vs OSA 1.14 ± 0.03 g, P = 0.08), RV free wall thickness (Sham 938 ± 55 μm vs OSA 876 ± 65 μm, P = 0.49), IVS thickness (Sham 2234 ± 162 μm vs OSA 2069 ± 190 μm, P = 0.54) or LV free wall thickness (Sham 2427 ± 178 μm vs OSA 2300 ± 190 μm, P = 0.64).
mRNA expression and protein synthesis
As a potential regulator of myocardial fibrosis in our model, protein levels of MMP-2 were analyzed in the four cardiac chambers. Representative blots and quantification are shown in Figure 3E. MMP-2 protein levels were lower in left atria from OSA-rats than in left atria from Sham-rats (p = 0.03), and showed borderline significance for the right atrium (p = 0.06). No differences were found between Sham and OSA groups in the left and right ventricles.
Mesenchymal stem cells prevent sleep apnea-induced atrial fibrosis
mRNA expression and protein synthesis
Results for MMP-2 mRNA expression in the atria were confirmed in protein levels quantification (Figure 8E). No differences were found in MMP-2 protein levels between Sham + S and OSA + MSC rats in the left or right atria. Contrary to mRNA expression, no differences were seen in MMP-2 protein levels in the LV (Sham + S vs OSA + MSC).
Figure 8D shows mean IL-1β levels in the Sham + S and OSA + MSC groups. OSA-induced increase in IL-1β plasma levels was blunted in the OSA group receiving MSC.
In the present work, we have demonstrated for the first time that chronic exposure of rats to recurrent apneas can promote atrial fibrosis, an established substrate for AF. This process seems to be mainly mediated through an increased local and systemic inflammation and a reduced collagen-degradation. Also, our data revealed that the OSA-induced fibrosis can be prevented by the infusion of MSC.
The application of intermittent hypoxia by modifying oxygen concentration in breathed gas, which is the most widely used model in OSA, is able to induce systemic inflammation. However, the setting employed in our work, in addition to intermittent hypoxia, allowed the application of increased negative intrathoracic-pressure swings . These increased inspiratory efforts could aggravate the early local inflammatory response triggered by intermittent hypoxia alone. In addition, both factors could participate in the development of AF by different mechanisms; i.e. intermittent hypoxia can elevate the systemic blood pressure but also, increased intrathoracic pressure swings can increase left ventricular transmural pressure  and may lead to repetitive atrial stretch which chronically could lead to left atrial enlargement and fibrosis.
Previous experimental studies reported enhanced AF-inducibility in acute OSA models, likely mediated by imbalances in the autonomic system [22, 23]. Iwasaki et al.  suggested a role for transient LA distension in AF substrate in an acute OSA model in obese rats. Few experimental works have studied the effects of OSA in cardiac structure at the histological level. Simpson et al.  showed that intermittent respiratory occlusions acutely induce multifocal areas of necrosis in both ventricles, and Chen et al.  described LV myocyte hypertrophy and apoptosis in a chronic intermittent hypoxia model. Neither of these two works studied atrial remodeling. To the best of our knowledge, our work is the first to describe the development of an arrhythmogenic structural substrate in the atria in a chronic OSA model.
Chamber-specific myocardial fibrosis (selective atrial fibrosis while preserving the ventricles) has been observed in our and other experimental settings [20, 27–29]. Various mechanisms explain increased atrial susceptibility to fibrosis. Differential stretch and mechanical-loading properties between the atria and ventricles , differences in atrial- and ventricular-fibroblast reactivity , and increased fibrotic response to myocardial ACE activity in the atria  have been reported and may contribute to atrial fibrotic susceptibility in our model.
The expression of the remaining collagen degradation (MMP-3, MMP-9, MMP-10, TIMP-1, TIMP-2) and collagen cross-linking (LOX) mediators were not affected in our model.
Besides a profibrotic effect, a growing core of evidence points to OSA as a pro-inflammatory disease . Previous work by our group showed that OSA induces a systemic inflammatory response in an acute animal model. This response was characterized by a significant increase in IL-1β plasmatic levels that was prevented by MSC infusion . The results of the present work confirm this finding in a chronic OSA model and show an increase in the pro-inflammatory cytokine IL-6 in both atria and the LV. Inflammation and fibrosis are closely linked, with several interrelated metabolic pathways. For example, IL-6 is involved in the development of myocardial fibrosis in other experimental settings . Moreover, plasma IL-1β selectively correlated to atrial fibrosis intensity, further highlighting higher fibroblast reactivity in the atria than in the LV . Interestingly, both systemic (IL-1β) and local (IL-6) inflammatory responses were prevented by MSC infusion. Notably, a growing core of evidence is suggesting that an anti-inflammatory effect underlies beneficial effects of MSC [12, 36].
Bone marrow MSC properties including pluripotency, avoidance of detection by the host immune system, and ease of expansion in culture make them an attractive option for cell therapy [13, 14]. Several studies have shown that MSC transplantation significantly decreases fibrosis in the heart [16, 17], lung , kidney , skin  and liver . Although the mechanisms whereby MSCs reduce tissue fibrosis remain unclear, previous studies support our finding that MSC-induced MMP-upregulation might be a hallmark of their antifibrotic effect. In mice skin, Wu et al.  demonstrated an antifibrotic effect of bone marrow MSC that was partially mediated by an increased MMP-2 collagen-degradation. Mias et al.  showed that MSC injection reduced ventricular fibrosis in a rat model of myocardial infarction by stimulating MMP-2 and MMP-9 secretion in fibroblasts. Consistent with a central role of MMP-2, MSC collagen-accumulation prevention was lost in MMP-2 knock-out fibroblasts . This effect is probably mediated by secreted factors in a paracrine/endocrine mechanism [15, 41]. Our data evidenced lack of MSC engrafted in the myocardium and support a systemic effect of MSC in this rat OSA-model.
The development of atrial fibrosis in this OSA model might have important clinical implications if confirmed in humans. First, it demonstrates that pro-arrhythmogenic remodeling can be directly caused by OSA and not limited to remodeling induced by other comorbidities such as hypertension, obesity, coronary artery disease, or diabetes, all of which are very prevalent in OSA patients . Accordingly, OSA screening might be important in AF-patients in order to establish a cause for the arrhythmia. Second, it may provide relevant prognostic information, as both OSA and atrial fibrosis predict poor outcomes of AF treatments. Finally, as OSA is a treatable disorder, early detection and treatment by means of CPAP or newer therapies may slow AF substrate progression and improve outcomes of antiarrhythmic drugs, electrical cardioversion, or AF ablation. Our results also suggest that MSC might have the potential to prevent the atrial profibrotic remodeling induced by OSA, but its clinical implication in humans needs to be demonstrated.
Some limitations should be acknowledged and taken into consideration when interpreting our results. First, we lack of electrophysiological and AF inducibility studies and thus, increased inducibility cannot be ensured from our results. However, we show that OSA induces atrial fibrosis, a hallmark of AF and a well-known AF promoter , at similar intensity to other reports showing increased inducibility . Second, we assessed protein levels for some genes, including collagen deposit, inflammatory cytokines and MMP-2, but failed to obtain blots for ACE. Third, we only assessed atrial fibrosis as AF substrate; additional studies focusing on atrial dilation or electrical remodeling are warranted.
In conclusion, our model demonstrates that OSA induces the development of atrial fibrosis, an arrhythmogenic substrate that might explain the association between OSA and atrial fibrillation. Fibrosis might be mediated by increased atrial expression of IL-6 and decreased atrial expression of MMP-2 with the subsequent decline in collagen-degradation. This atrial fibrosis is prevented by the intravenous administration of MSC, which normalizes IL-6 and MMP-2 expression.
The authors want to thank Neus Portella for editing assistance and Nadia Castillo for her excellent technical help.
This work was supported by a grant (PI10/00834) from the Fondo de Investigación Sanitaria and cofinanced by the ISCIII- Subdirección General de Evaluación and the European Regional Development Fund (FEDER); Ministerio de Economía y Competitividad (SAF2011-22576); REDINSCOR [V-2006-RET0308-O] and a grant from Hospital Clínic, Barcelona, Catalonia, Spain (Premi Emili Letang to PR).
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