Fig. 3
From: Detection of early-stage lung cancer in sputum using automated flow cytometry and machine learning
Heuristics-guided Viability Gate Setting. A Set a temporary flowClust gate on Non-debris events in FSC-H vs SSC-H to retain mostly live cells for eventual FVS510 tail gating (“core viable gate”). B For samples with < 10% events in the core viable gate, rerun flowClust more inclusively by increasing “quantile” parameter to 0.99. C Set a temporary singlets gate on core viable events, forcing the capture of the upper diagonal by setting the top right point to 2.5 × 105 on both axes. D A tail gate with 10% tolerance is set automatically on the core viable singlets (black histogram). Shown in blue is the full Non-debris FVS510 profile for comparison. The red bar indicates the viability gate cutoff. Viable events are to the left of the threshold. All temporary gates are removed once the threshold is determined