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Optimizing molecular testing of lung cancer needle biopsy specimens: potential solutions from an interdisciplinary qualitative study

Respiratory Research volume 24, Article number: 17 (2023) Cite this article

Abstract

Background

Molecular testing can detect actionable genomic alterations and tumor cell surface proteins in patients with non–small cell lung cancer (NSCLC). However, utilization remains suboptimal, representing missed treatment opportunities. This study aimed to identify challenges and potential solutions to obtaining percutaneous lung needle biopsy specimens for successful molecular testing in patients with advanced NSCLC.

Methods

This interdisciplinary qualitative study included ten radiologists and four pathologists from academic and community settings across the United States who routinely perform and analyze percutaneous lung needle biopsies. Participants underwent semi-structured one-on-one interviews (Phase 1). Interview questionnaires were constructed based on a literature review of key lines of inquiry and conducted by professional market researchers using the theoretical domains framework. Primary barriers to molecular testing were identified using thematic analysis. Subsequently, multidisciplinary focus groups were convened to identify potential solutions (Phase 2).

Results

Four themes emerged as barriers to molecular testing and were matched to the clinical workflow: (1) biopsy request, (2) biopsy procedure, (3) specimen analysis, and (4) communication. The nineteen potential solutions included adding a “checkbox” to indicate molecular testing in the biopsy request, leveraging pre-procedural imaging to guide biopsies, conserving tissue through appropriate allocation strategies and next generation sequencing panels instead of sequential single-gene assays, instituting reflex-molecular testing upon NSCLC diagnosis, tracking and communicating biopsy outcomes at multidisciplinary tumor boards, and improving integration of radiologists and pathologists into oncology care teams.

Conclusions

Potential solutions exist to increase successful molecular testing of lung needle biopsy specimens in patients with advanced NSCLC.

Introduction

Nearly three in four patients with non–small cell lung cancer (NSCLC) present with advanced disease at the time of diagnosis [1,2,3]. Sixty to 80% of those patients (Western versus Asian populations, respectively) harbor genomic alterations amenable to targeted therapy [4, 5]. Separately or in conjunction, patients may express tumor cell surface proteins rendering them eligible for immunotherapy [6, 7]. Patients with advanced NSCLC who receive first-line targeted therapy or immunotherapy have improved objective response rates [8, 9], longer progression-free survival, and longer overall survival [10,11,12,13,14,15]. Molecular testing (also referred to as biomarker testing, genomic analysis, molecular profiling, or molecular analysis) of lung needle biopsy specimens is crucial for identifying subsets of patients who are candidates for targeted therapy or immunotherapy. Despite recommendations by the National Comprehensive Cancer Network (NCCN) for molecular testing in all cases of advanced NSCLC [6], testing levels and results remain suboptimal [2, 16, 17], which translates to missed treatment opportunities [18].

While percutaneous lung needle biopsy is safe in the setting of advanced NSCLC [19, 20], confusion persists among radiologists and pathologists regarding how much tissue is needed [2], as well as the optimal preparation of tissue for histopathology and molecular testing [20, 21]. Insufficient material for molecular testing is procured approximately 40% of the time [8]. While efforts are underway to speed up molecular testing, the need for repeat biopsy can lengthen a patient’s wait to initiate therapy by an average of 57 days (range 31–90 days) [22, 23].

This study sought to identify real-world challenges and potential solutions to obtaining percutaneous lung needle biopsy specimens for successful molecular testing in patients with advanced NSCLC.

Methods

Study design

An interdisciplinary qualitative study consisting of semi-structured interviews and focus group discussions with radiologists and pathologists was conducted. Participants voluntarily agreed to have their interviews and focus group discussions transcribed and analyzed for the purposes of this study. The study was exempt from institutional review board approval [45 Code of Federal Regulations 46.104 (d) (2)].

Participants

The authors identified participants in an iterative process, targeting interventional and thoracic radiologists and pathologists across the United States who routinely perform and analyze percutaneous lung needle biopsies. Participants worked in academic or community-based clinical settings, and four were involved in developing the latest College of American Pathologists (CAP) thoracic biopsy guidelines [24]. Of 11 invited physicians, six agreed to participate. Eight additional radiologists and pathologists from community and academic sites in the southern and southeastern United States were invited to increase geographic and practice setting diversity. Seven out of eight agreed to participate. In total, 19 physicians were invited, and the final panel consisted of ten radiologists (six academic, four community) and four pathologists (two academic, two community). The median number of years in practice was 12 (range 3–32 years) (Figs. 1, 2).

Fig. 1
figure 1

Overview of Study Design and Participants Included

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Fig. 2
figure 2

Geographical overview of participants based on years of experience, type of practice, and speciality

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Data collection

Data collection occurred in two phases. In phase 1, trained moderators from the market research firm (Edge Research, Arlington, VA) conducted 14 semi-structured one-on-one 60-min interviews via videoconference from February to March 2021. Researchers developed lines of inquiry which included the following: goals of lung needle biopsy, awareness of molecular testing, typical lung biopsy scenarios and procedures, perceived and experienced challenges to obtaining tissue samples of sufficient quantity and quality for molecular testing, workflow, and communications between requesting providers and other team members, the role of liquid biopsy, and suggestions for improvement. The theoretical domains framework for the interview focused on knowledge, skills, intentions, context, resources, and consequences [25]. Questions are listed in Table 1. Some lines of questioning were directed only to radiologists; others were specifically directed to pathologists. An independent professional online transcription service recorded and transcribed all interviews.

Table 1 Guide for semi-structured one-on-one interviews (phase 1)
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In phase 2, two 90-min multidisciplinary focus groups were convened via videoconference in April and May 2021. The first group was comprised of six participants (four radiologists, two pathologists), and the second comprised of seven participants (six radiologists, one pathologist), all of whom had participated in the phase 1 interviews. Discussions were led by a trained moderator from the market research firm who presented findings from the semi-structured interviews and facilitated discussions to elicit divergent and convergent views regarding the interview findings and potential solutions (Table 2). One pathologist was unable to attend the focus groups and instead conducted a one-on-one discussion with the moderator (Table 2).

Table 2 Guide for focus group discussion (phase 2)
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Data analysis

The market researchers analyzed the transcripts of the recorded one-on-one interviews and focus groups to identify patterns for each discussion topic, applying tags to identify responses as a radiologist or pathologist perspective. Interview responses were sorted by question topic and further stratified by sub-topic. Researchers applied a thematic analysis to sort narrative text data into themes based on their relative frequency of appearance [26]. Each topic was examined for convergence or divergence with regard to their importance and impact on molecular testing among the panel. Based on the emerging themes, barriers and solutions were extracted and summarized directly as transcribed from the interviews and focus groups.

Results

Four themes emerged as barriers to obtaining percutaneous lung needle biopsy specimens for successful molecular testing in patients with advanced NSCLC and were matched to the clinical workflow as follows: (1) biopsy request, (2) biopsy procedure, (3) specimen analysis, and (4) communication (Fig. 3). Potential solutions to increase successful molecular testing of lung needle biopsy specimens were also matched to the clinical workflow (Table 3).

Fig. 3
figure 3

Overview of key barriers and potential solutions to obtaining percutaneous lung needle biopsy specimens for successful molecular testing. CT computed tomography, FNA fine needle aspiration, NGS next generation sequencing, NSCLC non-small cell lung cancer, ROSE rapid on-site evaluation

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Table 3 Potential solutions to increase comprehensive molecular testing of lung needle biopsy specimens in patients with advanced non-small cell lung cancer
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Biopsy request

Barriers

Radiologists reported lack of clarity regarding the goal of percutaneous lung needle biopsies. Most radiologists stated that they viewed lung biopsies primarily as a tool to confirm the diagnosis, histology, or staging of lung cancer. Some radiologists noted that the goal of a needle biopsy was to obtain tissue samples sufficient in both quantity and quality, and to avoid repeat biopsies. Although radiologists acknowledged the NCCN recommendations for molecular testing as part of the diagnostic workup of advanced NSCLC, molecular testing was still perceived as a secondary goal. Additionally, interviewees noted that biopsy requests were inconsistent in indicating the need for molecular testing.

Potential solutions

  • Educate referring clinicians that NSCLC workup requires comprehensive molecular testing through designated training modules or workshops.

  • Add a “checkbox” to the biopsy request in the electronic health record to indicate the request for molecular testing. Have the requesting provider check this box in all suspected and confirmed cases of advanced NSCLC.

  • Pre-screen lung needle biopsy requests and call the referring provider for clarification if the need for molecular testing is unclear.

Biopsy procedure

Barriers

Participants noted numerous barriers to obtaining tissue that is both high quality and of sufficient quantity: (1) limited pre-procedural imaging due to insurance coverage, (2) radiologist skill and comfort with image-guided lung needle biopsies, (3) lesion size, location, and density, (4) patient tolerance for the procedure, (5) lack of standardization for intraprocedural tissue evaluation, and (6) differing lab instructions for sample preparation. Participants cited being unsure of how much tissue was “enough.”

Participants differed in their opinions regarding the best approach for judging sample adequacy. While rapid on-site evaluation (ROSE) by a pathologist was described as the best practice in theory, radiologists noted that accurate assessment of a biopsy sample with minimal processing during ROSE requires extensive experience, lengthens the needle dwell time, increases the patient’s risk for complications, may not change the procedural steps for the radiologist, and does not appear to affect patient callback rate. Some pathologists noted that ROSE was not always necessary, may not be feasible in all cases, and may be more helpful for inexperienced radiologists. ROSE seemed more prevalent in academic settings due to pathologist availability. Most radiologists did not perform fine needle aspirations (FNA) and performed core biopsies only. Needle size was a matter of personal preference, and there was no consensus that larger needles were better for molecular testing.

Potential solutions

  • Review available imaging, including computed tomography (CT) and positron emission tomography (PET), prior to the procedure and stratify targets by imaging characteristics. For example, absence of 18-fluorodeoxyglucose uptake and intravenous contrast enhancement can identify necrotic tissue.

  • Utilize real-time (i.e., fluoroscopic) CT guidance during the procedure.

  • Obtain multiple tissue cores using coaxial technique while angling the biopsy device in all four quadrants

  • Target the periphery of large tumors to avoid central necrosis.

  • During ROSE, have the cytotechnologist verify that the specimen contains viable tumor cells.

  • Collect at least four tissue cores once ROSE confirms viable tumor cells.

  • Consider a higher tolerance for potential risk and obtain additional tissue cores if molecular testing is likely to affect survival, especially in suspected advanced NSCLC.

Specimen analysis

Barriers

Participants noted (1) a lack of awareness of current guidelines on molecular testing for patients with advanced NSCLC, (2) varying testing methods by lab, (3) varying sample requirements between multiple available testing platforms, and (4) rapidly changing best practices. Although most study participants were aware of the 2020 CAP thoracic biopsy guidelines, awareness of current NCCN guidelines for molecular testing was low. Some participants mentioned relying on institutional or departmental guidelines.

Potential solutions

  • Clarify how to allocate the specimens depending on institutional laboratory requirements.

  • Perform next generation sequencing (NGS) assays instead of sequential single-gene assays to conserve tissue and provide more comprehensive molecular testing.

  • If feasible, bank tissue for future molecular testing.

  • Institute “reflex-molecular testing” following histologic diagnosis of NSCLC to reduce turnaround time.

Communication

Barriers

Participants reported the absence of formal, consistent communication feedback loops between radiologists, pathologists, and clinical care teams regarding molecular testing results of lung needle biopsy specimens. Feedback was lacking on amount of viable tumor cells and molecular testing success unless a repeat biopsy was requested. Hospital-based radiologists can access biopsy results through the electronic medical record, while radiologists outside of hospital systems may not have access at all to the results.

Potential solutions

  • Track frequency of insufficient tissue, reasons for insufficiency, molecular testing results (especially true negatives versus insufficient samples), and complications from the biopsy procedure.

  • Integrate radiologists who perform lung needle biopsies more closely into the oncology care team and foster a culture of continuous feedback and follow-up (Fig. 4).

  • Include data-driven discussions of biopsy outcomes into multidisciplinary tumor boards.

  • Report amount of viable tumor cells in uniquely identified fields (not free-form comments) in the molecular testing report [27].

  • Institute patient navigators to facilitate follow-up on molecular testing outcomes.

Fig. 4
figure 4

Communication feedback loops to optimize molecular testing. ROSE rapid on-site evaluation

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Liquid biopsy

Panelists agreed that liquid biopsy does not obviate the need for molecular testing of tissue samples and is not currently sensitive enough to replace tissue sampling. However, panelists agreed that liquid biopsy would likely play a role in the future, such as in the setting of treatment failure. The panel was uncertain whether insurance companies would reimburse molecular testing of both tissue and liquid biopsies.

Discussion

This interdisciplinary qualitative study sought to identify challenges and potential solutions to obtaining percutaneous lung needle biopsy specimens for successful molecular testing in patients with advanced NSCLC. Four themes emerged as barriers and were mapped to the clinical workflow: (1) biopsy request, (2) biopsy procedure, (3) specimen analysis, and (4) communication. Nineteen potential solutions were identified and seem intuitive. This study is important because it identifies barriers and potential solutions to enhance molecular testing in patients with advanced NSCLC, a prerequisite to translate the discovery of novel targeted therapies and immunotherapy into potential cures [15].

Potential solutions identified in this study (Table 3) are in line with prior literature. A prior multidisciplinary panel of pathologists, interventional radiologists, oncologists and radiation oncologists concluded that pathologist-instituted reflex testing upon confirmation of non-squamous NSCLC can improve the timeliness of molecular testing [28]. The panel also highlighted the importance of collaboration and communication between physicians involved in diagnosing patients with NSCLC [28]. Gregg et al. offered similar strategies to improve molecular testing, including ordering molecular tests as soon as advanced NSCLC is suspected, conducting liquid biopsies early in the diagnostic pathway, and instituting pathology-directed reflex testing [29]. A recent report based on data from the Diaceutics Data Repository investigated implementation barriers to molecular testing in advanced NSCLC and identified clinical practice gaps at the stage of biopsy referral, biospecimen collection, biospecimen evaluation, biomarker test ordering, biomarker test performance, test result reporting, and treatment decisions. Specifically, the authors highlight that 136 out of 934 (14.5%) late stage NSCLC patients in the study are “lost” during the patient progression from diagnosis to treatment selection due to poor biospecimen collection [30].

Three potential solutions may be of interest for future implementation studies based on the projected low implementation cost. First, improving the biopsy request with the addition of a “checkbox” in the electronic health record to indicate the need for molecular testing would facilitate coordination between radiologists, pathologists, and referring providers. Knowing that molecular testing is the goal of the biopsy would affect the biopsy procedure and specimen processing in that radiologists would be prompted to collect more tissue and pathologists could optimize tissue processing. Second, as already studied by Lim et al. educating referring clinicians on the importance of molecular testing may increase testing rates [28]. Specialty-specific education programs reviewing data and guidelines on molecular testing previously achieved a 12% increase in molecular testing among diagnostic specialists in Ontario [10]. Third, creating feedback loops that present biopsy results (including true negatives versus insufficient samples) to radiologists and pathologists would help inform data-driven discussions of biopsy results and patient outcomes at multidisciplinary tumor boards.

Potential solutions in our study were geared towards crafting a framework that promotes successful molecular testing, including strategies to increase the yield of the biopsy procedure itself, which could be disseminated via workshops at specialty society meetings in addition to the literature. Biopsies should be performed using coaxial technique and target the periphery of large tumors to avoid central necrosis [31]. Review of pre-procedural imaging, such as fluorodeoxyglucose-PET and contrast-enhanced CT, facilitates the identification of necrotic areas [31]. The shorter procedure time afforded by real-time CT, often referred to as “CT fluoroscopy,” creates time for ROSE and the collection of additional samples [32]. A minimum of at least four tissue cores was recommended by the panel, exceeding the minimum of three recommended by the CAP guideline since research has demonstrated that sensitivity for histopathologic diagnosis and molecular testing increases significantly between the second and fourth samples [24, 33,34,35]. ROSE remains best practice according to the literature [36], despite the limitations noted by the panel. FNA was generally not performed but may have a role in facilitating ROSE [37]. However, touch preparations of tissue cores offer an alternative to ROSE based on fine needle aspirates, and use of touch preparations is supported by the CAP guidelines on handling lung needle biopsy specimens [24]. Limiting the need for re-biopsy was a valid concern among radiologists. However, it should be noted that repeat lung needle biopsies are generally safe and may be required to realize the potential offered by novel systemic therapies [19, 20, 38].

Potential solutions to optimize specimen analysis relate to tissue allocation techniques and testing methods. Herbst et al. advocate using minimal tissue for histology to conserve tissue for molecular testing and developing a histology protocol that allocates sections up front for molecular and immunohistochemistry analyses [39]. The National Cancer Institute Molecular Analysis for Therapy Choice trial similarly recommends splitting each core into two specimens, one for diagnosis and one for ancillary studies such as molecular testing [40]. During ROSE, pathologists and cytotechnologists should use specific terminology to distinguish between “diagnostic adequacy” versus “ancillary testing adequacy” [33]. Optical coherence tomography may, one day, be used to perioperatively quantify viable tumor cells versus scar in tissue cores [41]. NGS panels offer many advantages over sequential single-gene or “hotspot” assays (e.g., tests for only EGFR, ALK, and ROS1) [8]. Multiple “hotspot” assays can quickly exhaust available tissue and lead to false negatives due to tissue depletion [21, 29, 39, 42,43,44]. A recent study of advanced NSCLC found that NGS panels had a 39% lower rate of unsuccessful genotyping, resulting in 30% fewer missed treatment opportunities compared to non-NGS assays [18]. Although NGS may take several weeks to process, rapid EGFR testing workflows have been reported and allow treatment with EGFR-directed therapies within days, all without compromising NGS workflows [23]. Archiving tissue for future molecular testing was proposed as another potential solution; an important limitation to this approach is that tissues may lose antigenicity over time [2, 45], and characteristics of advanced tumors may change after treatment [46].

The limitations of this study should be interpreted within the context of its design and the absence of oncologists and pulmonologists. The questionnaires were designed by professional market researchers; this may have led to biases in the questionnaires influencing participant responses. However, by outsourcing the research, design, and execution of the study, inherent biases held by participants were avoided. Participation was voluntary; thus, the panel was susceptible to self-selection bias. The intentional selection of physicians who had helped develop the current CAP guidelines may have skewed responses, but the inclusion of academic and community practices provided a diverse perspective. Although the sample size was limited, participants from across the United States ensured geographic diversity.

Increased molecular testing is required to harness the benefits of novel targeted therapies and immunotherapy for patients with advanced NSCLC. Barriers and potential solutions to obtaining percutaneous lung needle biopsy specimens for successful molecular testing in patients with advanced NSCLC relate to the biopsy request, biopsy procedure, specimen analysis, and communication. As the number of therapies continues to grow, the need to overcome these barriers to performing molecular testing requires a coordinated effort among radiologists, pathologists, and referring clinicians. Implementation studies should aim to investigate the efficacy of the proposed potential solutions.

Availability of data and materials

Anonymized transcripts of participant interviews from EDGE Research is available upon request.

Abbreviations

CAP:

College of American Pathologists

CT:

Computed-tomography

NCCN:

National Comprehensive Cancer Network

FNA:

Fine needle aspiration

NSCLC:

Non-small cell lung cancer

PET:

Positron emission tomography

ROSE:

Rapid on-site evaluation

References

  1. Chen L, Smith DA, Somarouthu B, Gupta A, Gilani KA, Ramaiya NH. A radiologist’s guide to the changing treatment paradigm of advanced non-small cell lung cancer: the ASCO 2018 molecular testing guidelines and targeted therapies. Am J Roentgenol. 2019;213(5):1047–58.

    Article  Google Scholar 

  2. Conde E, Rojo F, Gómez J, et al. Molecular diagnosis in non-small-cell lung cancer: expert opinion on ALK and ROS1 testing. J Clin Pathol. 2022;75(3):145–53.

    Article  CAS  Google Scholar 

  3. American Lung Association. State of Lung Cancer 2020 report. Chicago IAJ, 2022. https://www.lung.org/getmedia/381ca407-a4e9-4069-b24b-195811f29a00/solc-2020-report-final.pdf.

  4. Hanna NH, Robinson AG, Temin S, et al. Therapy for stage IV non–small-cell lung cancer with driver alterations: ASCO and OH (CCO) joint guideline update. J Clin Oncol. 2021;39(9):1040–91.

    Article  CAS  Google Scholar 

  5. Tan AC, Tan DS. Targeted therapies for lung cancer patients with oncogenic driver molecular alterations. J Clin Oncol. 2022;40(6):611–25.

    Article  CAS  Google Scholar 

  6. Ettinger DS, Wood DE, Aisner DL, et al. Non-small cell lung cancer, version 3.2022, NCCN clinical practice guidelines in oncology. J Natl Compr Cancer Netw. 2022;20(5):497–530.

    Article  Google Scholar 

  7. Montero MA, Aricak O, Kis L, et al. Clinicopathological significance of the expression of PD-L1 in non-small cell lung cancer. Ann Diagn Pathol. 2021;51: 151701.

    Article  Google Scholar 

  8. Page RD, Drusbosky LM, Dada H, et al. Clinical outcomes for plasma-based comprehensive genomic profiling versus standard-of-care tissue testing in advanced non-small cell lung cancer. Clin Lung Cancer. 2022;23(1):72–81.

    Article  CAS  Google Scholar 

  9. Hellmann MD, Rizvi NA, Goldman JW, et al. Nivolumab plus ipilimumab as first-line treatment for advanced non-small-cell lung cancer (CheckMate 012): results of an open-label, phase 1, multicohort study. Lancet Oncol. 2017;18(1):31–41.

    Article  CAS  Google Scholar 

  10. Zer A, Cutz JC, Sekhon H, et al. Translation of knowledge to practice—improving awareness in NSCLC molecular testing. J Thorac Oncol. 2018;13(7):1004–11.

    Article  Google Scholar 

  11. John A, Yang B, Shah R. Clinical impact of adherence to NCCN guidelines for biomarker testing and first-line treatment in advanced non-small cell lung cancer (aNSCLC) using real-world electronic health record data. Adv Ther. 2021;38(3):1552–66.

    Article  Google Scholar 

  12. Hellmann MD, Paz-Ares L, Bernabe Caro R, et al. Nivolumab plus ipilimumab in advanced non–small-cell lung cancer. N Engl J Med. 2019;381(21):2020–31.

    Article  CAS  Google Scholar 

  13. Reck M, Rodríguez-Abreu D, Robinson AG, et al. Updated analysis of KEYNOTE-024: pembrolizumab versus platinum-based chemotherapy for advanced non–small-cell lung cancer with PD-L1 tumor proportion score of 50% or greater. J Clin Oncol. 2019;37(7):537–46.

    Article  CAS  Google Scholar 

  14. Arbour KC, Riely GJ. Systemic therapy for locally advanced and metastatic non–small cell lung cancer: a review. JAMA. 2019;322(8):764–74.

    Article  CAS  Google Scholar 

  15. Camidge DR, Doebele RC, Kerr KM. Comparing and contrasting predictive biomarkers for immunotherapy and targeted therapy of NSCLC. Nat Rev Clin Oncol. 2019;16(6):341–55.

    Article  CAS  Google Scholar 

  16. Makarem M, Ezeife DA, Smith AC, et al. Reflex ROS1 IHC screening with FISH confirmation for advanced non-small cell lung cancer—a cost-efficient strategy in a public healthcare system. Curr Oncol. 2021;28(5):3268–79.

    Article  Google Scholar 

  17. Steeghs EMP, Groen HJM, Schuuring E, et al. Mutation-tailored treatment selection in non-small cell lung cancer patients in daily clinical practice. Lung Cancer. 2022;167:87–97.

    Article  CAS  Google Scholar 

  18. Paz-Ares L, Gondos A, Saldana D, et al. Genomic testing among patients with newly diagnosed advanced non-small cell lung cancer in the United States: a contemporary clinical practice patterns study. Lung Cancer. 2022;167:41–8.

    Article  CAS  Google Scholar 

  19. Tongbai T, McDermott S, Fintelmann FJ, Price MC, Sharma A, Gilman MD. Complications and accuracy of computed tomography-guided transthoracic needle biopsy in patients over 80 years of age. J Thorac Imaging. 2019;34(3):187–91.

    Article  Google Scholar 

  20. Fintelmann FJ, Troschel FM, Kuklinski MW, et al. Safety and success of repeat lung needle biopsies in patients with epidermal growth factor receptor-mutant lung cancer. Oncologist. 2019;24(12):1570–6.

    Article  CAS  Google Scholar 

  21. Yu TM, Morrison C, Gold EJ, Tradonsky A, Layton AJ. Multiple biomarker testing tissue consumption and completion rates with single-gene tests and investigational use of oncomine dx target test for advanced non–small-cell lung cancer: a single-center analysis. Clin Lung Cancer. 2019;20(1):20-29.e28.

    Article  Google Scholar 

  22. Zhang Y, Shi L, Simoff MJ, Wagner OJ, Lavin J. Biopsy frequency and complications among lung cancer patients in the United States. Lung Cancer Manag. 2020;9(4):Lmt40.

    Article  CAS  Google Scholar 

  23. Dagogo-Jack I, Azzolli CG, Fintelmann F, et al. Clinical utility of rapid EGFR genotyping in advanced lung cancer. JCO Precis Oncol. 2018;2018:1.

    Google Scholar 

  24. Roy-Chowdhuri S, Dacic S, Ghofrani M, et al. Collection and Handling of Thoracic Small Biopsy and Cytology Specimens for Ancillary Studies: Guideline From the College of American Pathologists in Collaboration With the American College of Chest Physicians, Association for Molecular Pathology, America. Arch Pathol Lab Med. 2020;144(8):933–58.

    Article  CAS  Google Scholar 

  25. Atkins L, Francis J, Islam R, et al. A guide to using the Theoretical Domains Framework of behaviour change to investigate implementation problems. Implement Sci. 2017;12(1):77.

    Article  Google Scholar 

  26. Vaismoradi M, Turunen H, Bondas T. Content analysis and thematic analysis: implications for conducting a qualitative descriptive study. Nurs Health Sci. 2013;15(3):398–405.

    Article  Google Scholar 

  27. Wells C, Loshak H. CADTH Rapid Response Reports. Standardized Hospital Order Sets in Acute Care: A Review of Clinical Evidence, Cost-Effectiveness, and Guidelines. Ottawa (ON): Canadian Agency for Drugs and Technologies in Health

  28. Copyright © 2019 Canadian Agency for Drugs and Technologies in Health.; 2019.

  29. Lim C, Sekhon HS, Cutz JC, et al. Improving molecular testing and personalized medicine in non-small-cell lung cancer in Ontario. Curr Oncol. 2017;24(2):103–10.

    Article  CAS  Google Scholar 

  30. Gregg JP, Li T, Yoneda KY. Molecular testing strategies in non-small cell lung cancer: optimizing the diagnostic journey. Transl Lung Cancer Res. 2019;8(3):286–301.

    Article  CAS  Google Scholar 

  31. Sadik H, Pritchard D, Keeling D-M, et al. Impact of clinical practice gaps on the implementation of personalized medicine in advanced non–small-cell lung cancer. JCO Precis Oncol. 2022(6).

  32. Bourgouin PP, Rodriguez KJ, Fintelmann FJ. Image-guided percutaneous lung needle biopsy: how we do it. Tech Vasc Interv Radiol. 2021;24(3): 100770.

    Article  Google Scholar 

  33. Goiffon RJ, Best TD, Wrobel MM, et al. Reducing time and patient radiation of computed tomography-guided thoracic needle biopsies with single-rotation axial acquisitions: an alternative to “CT Fluoroscopy.” J Thorac Imaging. 2021;36(6):389–96.

    Article  Google Scholar 

  34. Beck KS, Kim T-J, Lee KY, Kim YK, Kang JH, Han DH. CT-guided coaxial biopsy of malignant lung lesions: are cores from 20-gauge needle adequate for histologic diagnosis and molecular analysis? J Thorac Dis. 2019;11(3):753–65.

    Article  Google Scholar 

  35. Jamshidi N, Huang D, Abtin FG, et al. Genomic adequacy from solid tumor core needle biopsies of ex vivo tissue and in vivo lung masses: prospective study. Radiology. 2016;282(3):903–12.

    Article  Google Scholar 

  36. Papadimitrakopoulou V, Lee JJ, Wistuba II, et al. The BATTLE-2 study: a biomarker-integrated targeted therapy study in previously treated patients with advanced non–small-cell lung cancer. J Clin Oncol. 2016;34(30):3638–47.

    Article  CAS  Google Scholar 

  37. Fassina A, Corradin M, Zardo D, Cappellesso R, Corbetti F, Fassan M. Role and accuracy of rapid on-site evaluation of CT-guided fine needle aspiration cytology of lung nodules. Cytopathology. 2011;22(5):306–12.

    Article  CAS  Google Scholar 

  38. VanderLaan PA. Percutaneous lung biopsy: point—fine-needle aspiration first. Am J Roentgenol. 2021;218(5):794–5.

    Article  Google Scholar 

  39. Yoon HJ, Lee HY, Lee KS, et al. Repeat biopsy for mutational analysis of non-small cell lung cancers resistant to previous chemotherapy: adequacy and complications. Radiology. 2012;265(3):939–48.

    Article  Google Scholar 

  40. Herbst RS, Aisner DL, Sonett JR, Turk AT, Weintraub JL, Lindeman NI. Practical considerations relating to routine clinical biomarker testing for non–small cell lung cancer: focus on testing for RET fusions. Front Med. 2021;7.

  41. Levy EB, Fiel MI, Hamilton SR, et al. State of the art: toward improving outcomes of lung and liver tumor biopsies in clinical trials—a multidisciplinary approach. J Clin Oncol. 2020;38(14):1633–40.

    Article  Google Scholar 

  42. Nandy S, Helland TL, Roop BW, et al. Rapid non-destructive volumetric tumor yield assessment in fresh lung core needle biopsies using polarization sensitive optical coherence tomography. Biomed Opt Express. 2021;12(9):5597–613.

    Article  CAS  Google Scholar 

  43. Sung S, Heymann JJ, Crapanzano JP, et al. Lung cancer cytology and small biopsy specimens: diagnosis, predictive biomarker testing, acquisition, triage, and management. J Am Soc Cytopathol. 2020;9(5):332–45.

    Article  Google Scholar 

  44. Waterhouse DM, Tseng W-Y, Espirito JL, Robert NJ. Understanding contemporary molecular biomarker testing rates and trends for metastatic NSCLC among community oncologists. Clin Lung Cancer. 2021;22(6):e901–10.

    Article  Google Scholar 

  45. Pennell NA, Arcila ME, Gandara DR, West H. Biomarker testing for patients with advanced non-small cell lung cancer: real-world issues and tough choices. Am Soc Clin Oncol Educ Book. 2019;39:531–42.

    Article  Google Scholar 

  46. Govindan R, Aggarwal C, Antonia SJ, et al. Society for Immunotherapy of Cancer (SITC) clinical practice guideline on immunotherapy for the treatment of lung cancer and mesothelioma. J Immunother Cancer. 2022;10(5): e003956.

    Article  Google Scholar 

  47. Yatabe Y, Dacic S, Borczuk AC, et al. Best practices recommendations for diagnostic immunohistochemistry in lung cancer. J Thorac Oncol. 2019;14(3):377–407.

    Article  CAS  Google Scholar 

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Acknowledgements

The study was supported by LUNGevity Foundation. The authors thank Speaking of Science for their support in preparing the manuscript. This project could not have been conducted without the research expertise of Lisa Dropkin, Mariel Molina, and Lydia Redway at Edge Research.

Guarantor statement

The authors (Fintelmann et al.) declare that they had full access to all the data in this study and the authors take complete responsibility for the integrity of the data and the accuracy of the data analysis.

Funding

The authors declare that they received support from LUNGevity Foundation for this study, which receives funding from pharmaceutical and diagnostic companies for its precision medicine programs, including Blueprint Medicines.

Author information

Authors and Affiliations

  1. Department of Radiology, Division of Thoracic Imaging and Intervention, Massachusetts General Hospital, 55 Fruit Street, Boston, MA, 02114, USA

    Florian J. Fintelmann & Ismail Tahir

  2. LUNGevity Foundation, Bethesda, MD, USA

    Nikki A. Martin

  3. Blueprint Medicines, Boston, MA, USA

    Elissa M. Quinn

  4. Beaumont Hospital, Royal Oak, MI, USA

    Timothy C. Allen

  5. Lowell General Hospital, Lowell, MA, USA

    Lija Joseph

  6. Wyoming Valley Radiology Associates, Wilkes-Barre General Hospital, Wilkes-Barre, PA, USA

    Boris Nikolic

  7. Department of Imaging, Cedars-Sinai Medical Center, Los Angeles, CA, USA

    Christopher Lee

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  1. Florian J. Fintelmann
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  2. Nikki A. Martin
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  3. Ismail Tahir
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  4. Elissa M. Quinn
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  5. Timothy C. Allen
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  6. Lija Joseph
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  7. Boris Nikolic
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  8. Christopher Lee
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Contributions

FJF, NAM, CL—Conceptualization, design, interpretation, writing, and editing. IT, EMQ, TCA, LJ, BN—Writing and editing. FJF, IT—Designing tables and figures. All authors were involved with revising the final manuscript prior to submission. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Florian J. Fintelmann.

Ethics declarations

Ethics approval and consent to participate

All participants consented to having their data included in the present study. Given the qualitative design, the study was exempt from formal institutional review board approval [45 Code of Federal Regulations 46.104 (d) (2)].

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Not applicable.

Competing interests

Lija Joseph is a consultant for Leica Biosystems. Nikki Martin owns shares in Roche. All other authors declare no relevant conflicts of interest.

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Fintelmann, F.J., Martin, N.A., Tahir, I. et al. Optimizing molecular testing of lung cancer needle biopsy specimens: potential solutions from an interdisciplinary qualitative study. Respir Res 24, 17 (2023). https://doi.org/10.1186/s12931-023-02321-9

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  • DOI: https://doi.org/10.1186/s12931-023-02321-9

Keywords

Respiratory Research

ISSN: 1465-993X

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