Fig. 3

Pharmacological targeting of SPI1 affects weight loss, airway inflammation, and airway remodeling. A Spi1 mRNA expression in PBS- and HDM-exposed mice. B Representative models of PBS (30 µL), HDM (25 µg/30 µL), HDM + DB1976 (1 mg/kg or 2.5 mg/kg) or HDM + DB2313 (1 mg/kg or 8 mg/kg) exposure intranasally 5 days/week for 3 weeks. C Percentage body weight of mice at the end of the model. D Total leukocytes, E macrophages, F neutrophils, G eosinophils and H lymphocytes in BALF. I AB-PAS staining for airway-associated mucus-secreting cells that were enumerated per µm of basement membrane. J Small airway collagen deposition measured using Sirius Red/Fast Green staining of lung sections and quantified per µm of basement membrane. All scale bars = 100 µm. Data expressed as mean ± SEM (n = 6–8 for each group). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, A: unpaired t-test, C-J one-way ANOVA, Sidak’s post hoc test