Fig. 1

Airway and parenchyma RNA-seq from HDM- and PBS-exposed mice reveals compartment- and exposure-specific gene expression. A HDM (25 µg/30 µL) or PBS (30 µL) was administered intranasally 5 days/week for 3 weeks. B Principal component analysis plot of HDM and PBS airway and parenchyma samples HDM (n = 10) and PBS (n = 12). Volcano plots of upregulated (red) and downregulated (blue) gene transcripts in airway (C) and parenchyma (D), dotted line represents P = 0.05. E Venn diagram of up- and down-regulated genes in the airways and parenchyma. F Gene enrichment analysis in the airways and parenchyma