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Fig. 4 | Respiratory Research

Fig. 4

From: d-Limonene inhibits the occurrence and progression of LUAD through suppressing lipid droplet accumulation induced by PM2.5 exposure in vivo and in vitro

Fig. 4

d-Limonene inhibited lipid droplets accumulation caused by PM2.5 exposure in vivo and in vitro. A Oil red O staining showed that PM2.5 exposure-induced lipid droplet formation was reduced after d-limonene treatment in LUAD cells. B Electron microscopic views of lipid droplets in normal lung epithelial cells (Beas-2b) and normal mouse lung tissue after long-term exposure to PM2.5. The number of lipid droplets decreased after treatment with d-limonene. C RNA-Seq analysis showed that PM2.5-induced upregulation of Srebf1, Fasn and Acaca was suppressed after treatment with d-limonene in mice. D, E PM2.5-induced upregulation of SREBF1, ACACA and FASN was inhibited by d-limonene intervention in A549 cell and C57BL/6 mice according to RT-qPCR. F Western blot analysis showed that PM2.5-induced upregulation of SREBF1, ACACA, and FASN was attenuated by treatment with D-limonene in Beas-2b cells and normal mouse lung tissue (N = 4, 5, 4). Scale bar = 500 nm. **P < 0.01; ***P < 0.001. CPM: C57BL/6 − PM2.5 exposure group; CPD: C57BL/6 − PM2.5 exposure + d-limonene group

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Respiratory Research

ISSN: 1465-993X

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