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Fig. 2 | Respiratory Research

Fig. 2

From: d-Limonene inhibits the occurrence and progression of LUAD through suppressing lipid droplet accumulation induced by PM2.5 exposure in vivo and in vitro

Fig. 2

Transcriptomic analysis showed that lipogenic genes were upregulated and lipid droplets were clearly detected after PM2.5 treatment of A549 and H1975 cells. A Heat map showing the differentially expressed genes between A549 cells treated with and without PM2.5 exposure. B KEGG results showing enrichment of the DEGs in the lipid metabolism pathway. C Results of transcriptional regulatory relationships according to sentence-based text mining. D Verification of the target genes by RT-qPCR. SBEBF1, FASN and ACACA were upregulated after PM2.5 exposure. E Compared to the untreated cells (blank), lipid droplets were substantially increased in A549 and H1975 after the cells were treated with PM2.5, as shown by electron microscope (Scale bar = 500 nm) and oil red O staining (Scale bar = 50 μm). AP A549-blank group, AB A549-PM2.5 exposure group. ***P < 0.001

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Respiratory Research

ISSN: 1465-993X

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