Fig. 2

Hsa_circ_0046264 overexpression down-regulated miR-1245 and up-regulated BRCA2. a The expression level of miR-1245 was higher in advanced lung cancer tissues (stage III and stage IV) than that in early-stage cancer tissues (stage I and stage II). ^*P < 0.05, compared with I + II (N = 9) group with lung cancer tissues in Stage I + II. b The expression level of BRCA2 was lower in early-stage lung cancer tissues than that in advanced cancer tissues. ^*P < 0.05, compared with I + II (N = 9) group with lung cancer tissues in Stage I + II. c The dual luciferase activity decreased in HEK293 cells co-transfected with hsa_circ_0046264 WT and miR-1245 mimics, but almost did not change in cells co-transfected with hsa_circ_0046264 MUT and miR-1245 mimics. ^*P < 0.05, compared with miR-con group. WT: wild type; MUT1: deletion mutation; MUT2: point mutation. d The dual luciferase activity decreased in HEK293 cells co-transfected with BRCA2 3’UTR WT and miR-1245 mimics, but almost not changed in cells co-transfected with BRCA2 3’UTR MUT and miR-1245 mimics. ^*P < 0.05 compared with miR-con group. (E, H) The relative expression of hsa_circ_0046264 increased in lung cancer cells transfected with the circ-vector pCD2.1-ciR (circ264 group). Control group was transfected with empty pLO-ciR vector. ^***P < 0.001, compared with control group. ^##P < 0.01, compared with circ264 group. f, i The relative expression of miR-1245 in lung cancer cells decreased in circ264 group and antimir1245 group but increased in sicirc groups and miR-1245 group. ^***P < 0.001, compared with control group. ^##P < 0.01, compared with circ264 group. g, j The expression of BRCA2 mRNA increased in circ-vector group. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, compared with control group. ^#P < 0.05, compared with circ264 group