- Letter to the Editor
- Open Access
Surfactant protein A mediates pulmonary clearance of Staphylococcus aureus
© Veith et al.; licensee BioMed Central Ltd. 2014
- Received: 20 February 2014
- Accepted: 20 July 2014
- Published: 5 August 2014
Surfactant protein A has been shown to enhance opsonization and clearance of Staphylococcus aureus in vitro. Here, the phagocytosis of alveolar S. aureus was investigated in vivo using intravital microscopy. Fluorescence labelled S. aureus Newman cells were intratracheally administered to anesthetized mice and the alveolar surface was observed for fifteen minutes. Confirming previously reported in vitro data, surfactant protein A-deficient mice showed a significantly reduced uptake of bacteria compared to wild-type mice.
- Alveolar Macrophage
- Surfactant Protein
- Intravital Microscopy
- Growth Phase Cell
The authors thank Ann Soether for editing the language. This study was supported by a grant of the German Ministry for Education and Research (BMBF) #01 KI 070103 and by a grant of the Deutsche Forschungsgemeinschaft (DFG) SFB-TR 84, TP C06.
- LeVine AM, Bruno MD, Huelsman KM, Ross GF, Whitsett JA, Korfhagen TR: Surfactant protein A-deficient mice are susceptible to group B streptococcal infection. J Immunol. 1997, 158: 4336-4340.PubMedGoogle Scholar
- Kudo K, Sano H, Takahashi H, Kuronuma K, Yokota S, Fujii N, Shimada K, Yano I, Kumazawa Y, Voelker DR, Abe S, Kuroki Y: Pulmonary collectins enhance phagocytosis ofMycobacterium aviumthrough increased activity of mannose receptor.J Immunol 2004, 172:7592–7602.,PubMedView ArticleGoogle Scholar
- Kuronuma K, Sano H, Kato K, Kudo K, Hyakushima N, Yokota S, Takahashi H, Fujii N, Suzuki H, Kodama T, Abe S, Kuroki Y: Pulmonary surfactant protein A augments the phagocytosis ofStreptococcus pneumoniaeby alveolar macrophages through a casein kinase 2-dependent increase of cell surface localization of scavenger receptor A.J Biol Chem 2004, 279:21421–21430.,View ArticleGoogle Scholar
- Sever-Chroneos Z, Krupa A, Davis J, Hasan M, Yang CH, Szeliga J, Herrmann M, Hussain M, Geisbrecht BV, Kobzik L, Chroneos ZC: Surfactant Protein A (SP-A)-mediated clearance ofStaphylococcus aureusinvolves binding of SP-A to the staphylococcal adhesion Eap and the macrophage receptors SP-A receptor 210 and scavenger receptor class A.J Biol Chem 2011, 286:4854–4870.,View ArticleGoogle Scholar
- Tschernig T, Veith NT, Schramm R, Laschke MW, Roller J, Rosenbruch M, Theegarten D, Bischoff M, Meier C, Menger MD: Direct visualisation of microparticles in the living lung. Exp Toxicol Pathol. 2013, 65: 883-886. 10.1016/j.etp.2012.12.007.PubMedView ArticleGoogle Scholar
- Hussain M, Haggar A, Heilmann C, Peters G, Flock JI, Herrmann M: Insertional inactivation of Eap inStaphylococcus aureusstrain Newman confers reduced staphylococcal binding to fibroblasts.Infect Immun 2002, 70:2933–2940.,PubMedPubMed CentralView ArticleGoogle Scholar
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