Anti-cytokine therapy in fibrosing alveolitis: where are we now?
© Current Science Ltd 2000
Received: 21 April 2000
Accepted: 12 June 2000
Published: 20 June 2000
Idiopathic pulmonary fibrosis (IPF) is a condition that has a poor prognosis, with a median survival of 4-5 years irrespective of treatment. Ziescheet al (N Engl J Med 1999,341: 1264-1269) describe an open randomised trial of 18 patients with IPF, unresponsive to corticosteroid treatment at high dose. Nine patients were treated with continued corticosteroid and nine with prednisolone plus interferon-γ 1b (IFN-γ). Significant benefits in physiological parameters are reported in the IFN-γ-treated group. An analysis of lung tissue by reverse-transcriptase-mediated polymerase chain reaction showed corresponding decreases in the transcription of transforming growth factor-β1 and connective tissue growth factor. This is the first report of treatment showing efficacy in this disease, albeit in a very preliminary study, but the data should be viewed with caution. This study is discussed in the context of other published studies of treatment for IPF and the scientific rationale on which it was based.
Idiopathic pulmonary fibrosis (IPF) is a chronic debilitating illness that leads inexorably to respiratory failure and death in most patients . The 5-year mortality approaches 50% in most studies . The efficacy of any treatment is questionable and this can lead to a rather nihilistic approach. Received wisdom suggests that this is a rare disease occurring mainly in elderly men and for which currently available treatment is often ineffective and can induce side effects that are worse than the condition itself. The symptoms of gradually increasing breathlessness are non-specific and often attributed to `old age', leading to presentation at a point that can be late in the natural history of the condition. Current therapy is usually corticosteroids with or without some form of immunosuppressant. However, the data upon which this is based are limited. The fact that these treatments are still widely used reflects the difficulty of a palliative approach for clinicians and patients in such a distressing condition.
The available studies of treatment for IPF are in small numbers of patients and are rarely controlled trials [3–14]. On reviewing these studies, it is apparent that some of the patients had collagen vascular disease and therefore did not have IPF. A significant number of the responders were less than 50 years old and were female, which is atypical. Histological data on these subjects were limited. The recent reclassification of interstitial lung diseases by histological features has shown a clear association between subtypes and response to treatment. Usual interstitial pneumonia (UIP) is the histological pattern that identifies IPF with little response to treatment [15,16]. Overall, these data suggest that those patients who responded to treatment in a previous trial might have had non-specific interstitial pneumonia (NSIP) rather than UIP. This exemplifies problems in the design and practice of appropriate trials in IPF owing to the relative rarity of the disease, the difficulties of including placebo controls, and the numbers of patients required to detect objective improvements.
Treatment with corticosteroids and immunosuppresive therapy is based on the concept that IPF is due to abnormalities in the immunoregulatory response, leading to progressive fibrosis rather than healing and resolution. These therapies have not been targeted at fibrogenic factors. There is a significant literature on the potential role of the fibrogenic factors transforming growth factor-β (TGF-β) and connective tissue growth factor (CTGF) in IPF [17,18]. It has also been demonstratedin vitro that several agents, including pirfenidone (5-methyl-1-phenyl-2-(1H)-pyridone) and IFN-γ, decreases their production [19,20]. The use of these agents in therapeutic trials in IPF is an exciting prospect because potentially they are affecting fibrogenesis, which can be considered a downstream aspect in the pathogenesis of IPF, a common point for clinical presentation.
One study has previously reported on the anti-fibrotic agent pirfenidone in patients with IPF . This showed some limited improvement and was performed in patients with advanced diseases. Ziescheet al  have reported an open clinical trial of IFN-γ in patients with IPF. Patients were excluded if they had a total lung capacity [TLC] of less than 45% of predicted and were regarded as end stage. Patients were considered eligible if they showed no response to corticosteroid at high dose (50 mg daily for 4 weeks), with response defined as an improvement in TLC of 10%. Eighteen subjects were recruited, nine were treated with prednisolone at low dose (7.5 mg/day) and compared with nine treated with IFN-γ (200 μg, three times daily) and prednisolone (7.5 mg/week). Significant changes were reported in the IFN-γ-treated group after 12 months. Three physiological endpoints were described. Total lung capacity had decreased by 4% in the control group (from 66 ± 8% to 62 ± 6% of predicted) compared with an increase of 9% in the IFN-γ group (70 ± 10% to 79 ± 12% of predicted). The resting partial pressure of arterial oxygen (PaO2) decreased in the control group from 65 ± 6 to 62 ± 4 mmHg compared with an increase in the IFN-γ group from 64 ± 9 to 76 ± 8 mmHg, with comparable improvements in post-exercise PaO2. No data were reported on cigarette smoking, which is known to have an effect on outcome .
Transcriptional changes in TGF-β1 and CTGF, measured by semiquantitative RT-PCR (reverse-transcriptase-mediated polymerase chain reaction), were also demonstrated in the IFN-γ group. These data permit speculation on the mechanisms responsible for these documented improvements. These data are promising but must be treated with caution . The patients studied were identified by clinical, radiographic (including high-resolution computed tomography), physiological parameters and histological data. Fifteen had undergone open lung biopsy and three had undergone transbronchial biopsy; IPF was diagnosed on the basis of the presence of subpleural and periacinar fibrotic lesions with minor cellular infiltration. The recent histological reclassification of IPF shows a very significant relationship between subtype and both mortality and response to treatment, as mentioned previously . The data would be significantly strengthened if the authors now had a histological review by an independent pathological expert. In addition to these histological criteria, the patients had to have shown a deterioration of a minimum of 10% decrease in lung function in the preceding 12 months despite treatment with corticosteroid and/or immunosuppressive agents for at least 6 of the preceding 12 months. The patients had a mean age of 61 years, which is young for this disease. At the start of the study the mean values of TLC were 66 ± 6% and 70 ± 10% of predicted in the two groups, which is a level that is rarely seen in the outpatient setting in the UK. An increase of 10% or 200 ml in TLC is required for an improvement to be considered significant. The authors have replied to comments on this issue, pointing out that in view of the relatively well preserved TLC in their subjects there was a mean increase of approximately 50 ml in their subjects . The figures for gas transfer are not given, which is an important physiological measure that is regarded by some authorities as the most crucial in monitoring. PaO2 values before and after maximal exercise were used as surrogate markers of gas exchange; however, no details of the exercise performed are given, so corrections for work performed cannot be made. Furthermore the 5-year mortality for this disease is in the region of 50%, but there were no deaths in the 18 patients despite a total of seven patients requiring oxygen therapy. The patients described in this study are unusual in their `physiological health' at the entry to the study and lack of deterioration in the course of the study. This leads to a question of whether their apparent response to IFN-γ can be considered representative of IPF, in particular of the UIP subset, by contrast with those with the more responsive NSIP subset. Further studies of this agent are warranted but the problem of patient selection for studies of IPF remains unresolved: on the one hand, the stricter the criteria the more difficult recruitment becomes; on the other hand is the large number required to detect significant clinical outcomes.
This study gives us a glimmer of hope, firstly to raise the profile of IPF as a disease requiring more and very well designed clinical trials, and secondly as the second study to look at agents that are potentially antifibrotic, targeting the process that leads to the disability of IPF.
- The American Thoroacic Society and the European Thoracic Society :Idiopathic pulmonary fibrosis: diagnosis and treatment: international consensus statement. Am J Respir Crit Care Med 2000,161:646–664.View ArticleGoogle Scholar
- Panos RJ, Mortenson R, Niccoli SA, King Jr TE:Clinical deterioration in patients with idiopathic pulmonary fibrosis: causes and assessment. Am J Med 1990,88:396–404.View ArticlePubMedGoogle Scholar
- Turner-Warwick M, Burrows B, Johnson A:Cryptogenic fibrosing alveolitis: response to corticosteroid treatment and its effect on survival. Thorax 1980,35:593–599.View ArticlePubMedPubMed CentralGoogle Scholar
- Turner-Warwick M, Burrows B, Johnson A:Cryptogenic fibrosing alveolitis: clinical features and their influence in survival. Thorax 1980,35:171–180.View ArticlePubMedPubMed CentralGoogle Scholar
- Rudd RM, Haslam PL, Turner-Warwick M:Cryptogenic fibrosing alveolitis: relationship of pulmonary physiology and bronchoalveolar lavage to response to treatment and prognosis. Am Rev Respir Dis 1981,124:1–8.PubMedGoogle Scholar
- Johnson MA, Kwan S, Snell NJC, Nunn AJ, Darbyshire JH, Turner-Warwick M:Randomised controlled trial comparing prednisolone alone with cyclophosphamide and low dose prednisolone in combination in cryptogenic fibrosing alveolitis. Thorax 1989,44:280–288.View ArticlePubMedPubMed CentralGoogle Scholar
- Winterbauer RH, Hammar SP, Hallman KO, Hays JE, Pardee NE, Morgan EH, Allen JD, Moores KD, Bush W, Walker JH:Diffuse interstitial pneumonitis: clinicopathologic correlations of 20 patients treated with prednisone/azathioprine. Am J Med 1989,65:661–672.View ArticleGoogle Scholar
- Raghu G, Depaso WJ, Cain K, Hammar SP, Wetzel CE, Dreis DF, Hutchinson J, Pardee NE, Winterbauer RH:Azathioprine combined with prednisone in the treatment of idiopathic pulmonary fibrosis: a prospective double-blind, randomised, placebo-controlled trial. Am Rev Respir Dis 1991,144:291–296.View ArticlePubMedGoogle Scholar
- Douglas WW, Ryu JH, Swensen SJ, Offord KP, Schroeder DR, Caron GM, DeRemee RA:Colchicine versus prednisone in the treatment of idiopathic pulmonary fibrosis: a randomised prospective study. Am J Respir Crit Care Med 1998,158:220–225.View ArticlePubMedGoogle Scholar
- Dayton CS, Schwartz DA, Helmers RA, Pueringer RJ, Gilbert SR, Merchant RK, Hunninghake GW:Outcome of subjects with idiopathic pulmonary fibrosis who fail corticosteroid therapy: implications for future studies. Chest 1993,103:69–73.View ArticlePubMedGoogle Scholar
- Schwartz DA, Helmers RA, Galvin JR, Van Fossen DS, Frees KL, Dayton CS, Burmeister LF, Hunninghake GW:Determinants for survival in idiopathic pulmonary fibrosis. Am J Respir Crit Care Med 1994,149:450–455.View ArticlePubMedGoogle Scholar
- Baughman RP, Lower EE:Use of intermittent, intravenous cyclophosphamide for idiopathic pulmonary fibrosis. Chest 1992,102:1090–1094.View ArticlePubMedGoogle Scholar
- Mapel DW, Samet JM, Coultas DB:Corticosteroids and the treatment of idiopathic pulmonary fibrosis: past, present and future. Chest 1996,110:1058–1067.View ArticlePubMedGoogle Scholar
- Selman M, Carrillo G, Salas J, Padilla RP, Perez-Chavira R, Sansores R, Chapela R:Colchicine, d-penicillamine, and prednisone in the treatment of idiopathic pulmonary fibrosis: a controlled clinical trial. Chest 1998,114:507–512.View ArticlePubMedGoogle Scholar
- Katzenstein AA, Meyers JL:Idiopathic pulmonary fibrosis: clinical relevance of pathologic classification. State of the art. Am J Respir Crit Care Med 1998,157:1301–1315.View ArticlePubMedGoogle Scholar
- Bjoraker JA, Ryu JH, Edwin MK, Myers JL, Tazelaar HD, Schroeder DR, Offord KP:Prognostic significance of histopathologic subjects of idiopathic pulmonary fibrosis. Am J Respir Crit Care Med 1998,157:199–203.View ArticlePubMedGoogle Scholar
- Broekelmann TJ, Limper AH, Colby TV, McDonald JA:Transforming growth factor beta-1 is present at sites of extracellular matrix gene expression in human pulmonary fibrosis. Proc Natl Acad Sci USA 1991,88:6642–6646.View ArticlePubMedPubMed CentralGoogle Scholar
- Grotendorst GR:Connective tissue growth factor: a mediator of TGF-beta action on fibroblasts. Cytokine Growth Rev 1997,8:171–179.View ArticleGoogle Scholar
- Lurton JM, Trejo T, Narayaman AS, Raghu G:Pirfenidone inhibits the stimulatory effects of profibrotic cytokines on human lung fibroblasts in-vitro [abstract]. Am J Respir Crit Care Med 1996,153:A403.Google Scholar
- Kasama T, Strieter RM, Lukacs NW, Lincoln PM, Burdick MD, Kunkel SL:Interferon gamma modulates the expression of neutrophil-derived chemokines. J Invest Med 1995,43:58–67.Google Scholar
- Raghu G, Johnson G, Lockhart D, Mageto Y:Treatment of idiopathic fibrosis with a new anti-fibrotic agent, pirfenidone: results of a prospective, open label phase II study. Am J Respir Crit Care Med 1999,159:1061–1080.View ArticlePubMedGoogle Scholar
- Ziesche R, Hofbauer E, Wittmann K, Petkov V, Block LH:A preliminary study of long-term treatment with interferon gamma-1b and low-dose prednisolone in patients with idiopathic pulmonary fibrosis. N Engl J Med 1999,341:1264–1269.View ArticlePubMedGoogle Scholar
- Baumgartner KB, Samet JM, Stidley CA, Colby TV, Waldron JA:Cigarette smoking: a risk factor for idiopathic pulmonary fibrosis. Am J Respir Crit Care Med 1997,155:242–248.View ArticlePubMedGoogle Scholar
- King TE:Interferon gamma-1b for the treatment of idiopathic pulmonary fibrosis [letter]. N Engl J Med 2000,342:974.View ArticlePubMedGoogle Scholar
- Ziesche R, Block LH:Interferon gamma-1b for the treatment of idiopathic pulmonary fibrosis [letter]. N Engl J Med 2000,342:975.View ArticleGoogle Scholar