Iron homeostasis and oxidative stress in idiopathic pulmonary alveolar proteinosis: a case-control study
© Ghio et al. 2008
Received: 04 September 2007
Accepted: 23 January 2008
Published: 23 January 2008
Lung injury caused by both inhaled dusts and infectious agents depends on increased availability of iron and metal-catalyzed oxidative stress. Because inhaled particles, such as silica, and certain infections can cause secondary pulmonary alveolar proteinosis (PAP), we tested the hypothesis that idiopathic PAP is associated with an altered iron homeostasis in the human lung.
Healthy volunteers (n = 20) and patients with idiopathic PAP (n = 20) underwent bronchoalveolar lavage and measurements were made of total protein, iron, tranferrin, transferrin receptor, lactoferrin, and ferritin. Histochemical staining for iron and ferritin was done in the cell pellets from control subjects and PAP patients, and in lung specimens of patients without cardiopulmonary disease and with PAP. Lavage concentrations of urate, glutathione, and ascorbate were also measured as indices of oxidative stress.
Lavage concentrations of iron, transferrin, transferrin receptor, lactoferrin, and ferritin were significantly elevated in PAP patients relative to healthy volunteers. The cells of PAP patients had accumulated significant iron and ferritin, as well as considerable amounts of extracellular ferritin. Immunohistochemistry for ferritin in lung tissue revealed comparable amounts of this metal-storage protein in the lower respiratory tract of PAP patients both intracellularly and extracellularly. Lavage concentrations of ascorbate, glutathione, and urate were significantly lower in the lavage fluid of the PAP patients.
Iron homeostasis is altered in the lungs of patients with idiopathic PAP, as large amounts of catalytically-active iron and low molecular weight anti-oxidant depletion are present. These findings suggest a metal-catalyzed oxidative stress in the maintenance of this disease.
Pulmonary alveolar proteinosis (PAP; also alveolar proteinosis and alveolar lipoproteinosis) has been classified as a disease entity since 1958 . PAP is characterized by the massive accumulation of surfactant in the airspaces of the lower respiratory tract [2, 3]. Pulmonary surfactant is a mixture of phospholipids and proteins essential for lung homeostasis and alveolar stability. The accumulation of surfactant in alveolar proteinosis appears to result from impaired phospholipid clearance rather than over-production by type II pneumocytes . PAP is also characterized by inflammation with accumulation of abnormal alveolar macrophages containing intracellular surfactant-like material, and these phagocytes demonstrate impaired function [5, 6]. Hyperplasia of type II pneumocytes, infiltration of lymphocytes and fibroblasts, and fibrosis can also be observed in PAP [7–9].
In most cases, PAP is idiopathic or primary, but secondary PAP can accompany inhalational dust exposures (e.g. silica), infections (especially Mycobacteria, pneumocystis carinii, nocardia, and cryptococci), malignancies, and immunosuppressive disorders [10, 11]. The etiological factors in primary PAP remain unknown, but the development of antibodies to GM-CSF has led to the postulate that it is an auto-immune disorder . Lung damage from infectious agents and inhaled dusts is promoted by the availability of iron and metal-catalyzed oxidative stress [12, 13]. We tested the hypothesis that primary PAP can be associated with altered iron homeostasis in the lung by measuring concentrations of iron and iron-related proteins in lavage from both patients with idiopathic PAP and healthy control subjects.
Bronchoalveolar lavage in healthy subjects and patients with PAP
Alveolar lavage fluid was obtained from healthy volunteers by bronchoscopy with bronchoalveolar lavage (BAL) at the U.S. Environmental Protection Agency in Chapel Hill, NC under a protocol approved by the University of North Carolina . Topical 2% lidocaine was used to anesthetize the upper airway and larynx. A fiberoptic bronchoscope was passed into the trachea, where 1% lidocaine was used for topical anesthesia. The lingula of the left lung was accessed, where the bronchoscope was wedged into a subsegment. BAL was performed with 250 mL of 0.9% saline injected serially in 50 mL aliquots. This procedure was repeated in the right middle lobe. Indices of iron homeostasis were measured in pooled samples of lavage fluid from the third, fourth, and fifth aliquots.
Lavage fluid was collected from patients with idiopathic PAP undergoing whole lung lavage for symptomatic treatment at the Duke University Medical Center [6, 11]. The study protocol was approved by the Duke University Institutional Review Board. There was no clinical evidence of infection, including fever, chills, myalgias, elevated temperature, and leukocytosis, in any patient. Procedures were performed under general anesthesia using independent lung ventilation with a dual-lumen endotracheal tube. While the left lung was ventilated with 100% O2, the right lung was filled with warm 0.9% NaCl at 37°C. After drainage of 500 mL of lavage fluid, serial exchanges of 500 mL each were made using gravity augmented by chest physical therapy. Lavage was continued until the fluid was clear, which usually occurred after 15 liters. Samples used in this investigation were obtained from the second 1.5 L aliquot of collected fluid.
Lavage iron, tranferrin, transferrin receptor, lactoferrin, ferritin, and protein
Since iron is taken up and released by all lung cells , iron homeostasis is maintained by an array of iron handling proteins, redox reactions, and storage mechanisms. Isolated parameters of iron content are not definitive; therefore a battery of measurements of uptake, storage and release proteins is needed to assess iron homeostasis. We measured lavage concentrations of iron, as well as levels of transferrin, transferrin receptor, lactoferrin, and ferritin as indices of the metal's homeostasis. In addition, iron and ferritin stains were performed in both lavage cells and lung tissue.
Iron concentration was measured with a standard colorimetric assay (Sigma, St. Louis, MO). Transferrin concentrations in lavage supernatants were analyzed using a commercially available kit, controls, and standards from INCSTAR Corporation (Stillwater, MN). TfR and ferritin were measured with commercial ELISA kits (R & D Systems, Minneapolis, MN) as were lactoferrin concentrations (Calbiochem, La Jolla, CA). Lavage protein was determined with Coomassie Plus Protein Assay Reagent (Pierce, Rockford, IL) using bovine serum albumin as the standard.
Iron staining and ferritin immunohistochemistry
Lavage cells (at 1.0 × 106/1.0 mL) were cytocentrifuged (0.2 mL) onto slides and stained for both iron and ferritin using Perl's Prussian Blue for iron (n = 4 for healthy subjects and 4 for PAP patients; all PAP cytospins were collected from non-smokers). Immunohistochemistry for ferritin was performed as reported with rabbit anti-human ferritin primary antibody (Dako, Carpinteria, CA) .
Human lung specimens were obtained from autopsy archives at Duke University Medical Center (fixed in 10% formalin). Immunohistochemistry was done on specimens from patients with primary PAP and specimens from patients with no lung disease listed as a diagnosis on the autopsy (n = 4 for no lung disease and 4 for PAP; all blocks were collected from non-smokers). Tissue sections were cut, floated on a protein-free water bath, mounted on silane treated slides (Fisher, Raleigh, NC), and air-dried overnight. Sections were stained for iron using Perl's Prussian Blue. Immunohistochemistry for ferritin was performed using an human anti-ferritin from Dako .
Measurement of ascorbate, glutathione, and urate
Lavage samples were acidified by adding 35 μL of 60% perchloric acid to 1.0 mL of lavage and 1.5 mL of 4% percholoric acid to 0.5 mL of plasma. After 30 min centrifugation at 20,000 g, the supernatant was assayed for ascorbate and urate using high performance liquid chromatography (Waters RCM μ BondaPak C18 column, Millipore Corporation, Marlborough, MA) with electrochemical detection (BAS model LC-4B, Bioanalytical Systems, W. Lafayette, IN) . Total glutathione was measured in the supernatant by quantifying nonprotein sulfhydryls, .
Data are expressed as mean values ± standard deviations. Differences between two groups were compared using T-tests of independent means. Significance was assumed at p < .05.
The healthy subjects that underwent a research bronchoscopy and bronchoalveolar lavage included 14 male and 6 female with a mean age of 26.5 +/- 3.3 years; all were non-smokers. In the group of patients with idiopathic PAP undergoing therapeutic whole lung lavage, there were 17 males and 3 females. The mean age in this cohort was 41.6 +/- 8.3 years; significantly older than the normal subjects. Among the PAP patients, there were 13 smokers/ex-smokers and 7 non-smokers.
The pooled BAL fluid from healthy volunteers was clear while that collected from patients with PAP was cloudy and ranged in color from beige to light pink. The amount of protein in the lavage collected from PAP patients was nearly ten-fold higher than that of the healthy volunteers (1,017 +/- 281 vs. 101 +/- 13 μgrams per mL).
We also measured ferritin, which stores iron in a non catalytically-reactive form. Iron sequestration by ferritin limits its capacity to generate free radicals and confers an antioxidant function to the protein [25, 26]. Ferritin concentration in the normal lung is high, reflecting the direct interface of the organ with iron particulates in the external environment . We found measurable concentrations of ferritin in BAL fluid from normal subjects, while ferritin levels in lavage fluid of PAP patients were unusually high (Figure 3B). Moreover, the ferritin levels measured in BAL fluid in PAP greatly exceed the normal serum levels of ferritin in humans (20 to 200 ng/mL).
In analyses of results from PAP patients only, there were no significant differences between smokers/ex-smokers, and non-smokers in lavage concentrations of iron, transferrin, transferrin receptor, lactoferrin, and ferritin.
This investigation describes a prominent disorder of iron homeostasis in the lower respiratory tract of individuals with primary PAP. Alterations in iron metabolism were evidenced by elevations in both concentrations of iron and levels of transferrin, transferrin receptor, lactoferrin, and ferritin in all patients with PAP. Histology and immunohistology also confirmed excess accumulation of iron and ferritin in the lungs of these patients. Corresponding to this iron accumulation, levels of ascorbate, urate, and glutathione were depressed consistent with the presence of oxidative stress. These changes in iron homeostasis and oxidative stress are not attributed to an increase in vascular permeability among patients with PAP; aberrant transport through a damaged blood-air barrier has been demonstrated not to occur in alveolar proteinosis .
Lung injury following exposures to both infectious agents and particles/fibers are iron-dependent [12, 13]. Notably, surfactant accumulation in PAP is associated with certain infections and with inhalation of some particulates. This association suggested that iron homeostasis might be abnormal in PAP. A relationship between iron homeostasis, oxidative stress, and altered accumulation and function of surfactant has been suggested by prior investigations. For example, iron-loaded transferrin inhibits surfactant function in the airways of rabbits with acute respiratory failure, while apotransferrin has no effect on surfactant function . In addition, iron-loaded transferrin incubated with H2O2 and a reducing agent leads to oxidative inactivation of surfactant, while transferrin without iron has the opposite, antioxidant effect . Finally, administration of holotransferrin enhances acute respiratory failure in surfactant-depleted rabbits  while administration of apotransferrin is beneficial in the same animal model . As the only difference between the exposures to holotransferrin and apotransferrin is the presence or absence of bound iron, it can be concluded that the metal presents an oxidative stress that leads to decrements in surfactant function and worsens respiratory failure.
Surfactant accumulation in rats after silica exposure also correlates with the concentration of iron complexed to the surface of the mineral oxide , supporting the association of metal-catalyzed oxidative stress with surfactant metabolism. Other mineral oxide dusts, including cristobalite, chrysotile, and kaolinite, have a comparable capacity to coordinate iron and present an oxidative stress to the lower respiratory tract. Similarly, these particles can be associated with elevated levels of extracellular surfactant . Surfactant-enriched material appears to have a capacity to function as an in vitro target for oxidants catalyzed by Fe3+ complexed to the surface of a particle .
Accumulation of surfactant in the lower respiratory tract occurs following exposure to other forms of oxidative stress which are also associated with an altered iron homeostasis. For example, environmental oxidants such as ozone and nitrogen dioxide increase the alveolar surfactant concentration [31, 32]. In addition, chelates and metals other than iron can either redox cycle or disrupt iron metabolism, leading to oxidative stress and elevated surfactant levels in the lung [33–35]. Certain respiratory infections, such as tuberculosis and nocardiosis, are also causes of localized alveolar proteinosis, and our group has previously reported that a substantial fraction of patients with primary PAP have positive BAL cultures for Mycobacterium avium complex (11).
An alteration of iron homeostasis and oxidative stress are found in the lung during certain infections and particle/fiber exposures, and for the first time we report idiopathic PAP among the lung disorders with disturbed iron handling. That both oxidative stress and altered iron homeostasis are present in the lungs of patients with PAP is associative; however, a causal link is strongly suggested by the fact that PAP is closely associated with stimuli that create significant oxidative stress in the lower respiratory tract. Oxidative injury following the exposure of the lung to microorganisms or particulates could contribute to disease pathogenesis in primary PAP by causing surfactant oxidation and dysfunction, and its subsequent accumulation .
An accumulation of iron in lung tissue with aging has been demonstrated . Some increase in lavage iron is certainly predicted with aging but this has not been confirmed in any study. Based on these reported changes in tissue non-heme iron concentrations, the differences in age observed in this study between the healthy subjects and PAP patients cannot account for the observed disparities in iron and iron-related proteins. Relative to aging, smoking will have a greater impact on lavage concentrations of iron and iron-related proteins. Iron concentrations also increase in the lavage from cigarette smokers . Concentrations of specific iron-related proteins in lavage fluid have not been reported except for ferritin and this storage protein increased in the lavage fluid of smokers [37, 38]. Smoking may contribute to some portion of the differences in iron and iron-related proteins observed in this study between the healthy subjects and PAP patients. However, smoking fails to explain disparities between these two cohorts as there were no significant differences in lavage concentrations of iron and iron-related proteins between smokers/ex-smokers and non-smokers among the PAP patients. In addition, the magnitude of changes observed reported in previous studies among smokers does not approach those observed in this investigation among PAP patients.
Lavage iron and ferritin concentrations are similarly elevated among patients with acute respiratory distress syndrome  and pneumoconiosis, and following transplantation . However, the disturbance in iron homeostasis among PAP patients reported in this investigation is exceptional in its magnitude with 5- to 20-fold elevations in concentrations of the metal and specific iron-related proteins.
Since reactive oxygen species (ROS) generated by excess iron damages biological molecules, optimal homeostasis requires extremely low concentrations of free iron. The transport and storage of iron is with all coordination sites complexed to prevent excess ROS generation. The host responds to elevated concentrations of available iron by sequestering it in the storage protein ferritin. This requires transporting the metal intracellularly, usually by the glycoprotein transferrin, which uses a membrane-bound receptor TfR. Lactoferrin-mediated metal transport is an alternative mechanism for metal transfer after exposures to microbes and particles/fibers, and is important for transport of iron to ferritin in monocytes and macrophages during inflammation. A novel aspect of our data is the finding that TfR in PAP lavage fluid is increased despite elevated soluble iron levels in the lung. With exposure to iron, many cell types rapidly down regulate TfR expression to limit iron uptake. However, with elevations in available iron observed during inflammation, monocytes and macrophages actually increase TfR expression [42, 43]. Increased TfR in the BAL of PAP patients may reflect a defensive attempt to transport and sequester elevated concentrations of metal that occur in inflammatory injuries.
We conclude that primary PAP is associated with an altered iron homeostasis in the lung. Our findings suggest the presence of metal-catalyzed oxidative stress in this rare lung disease. They also imply that, rather than being a uniform disease with a single etiology, PAP can be a pattern of injury associated with loss of iron homeostasis in the lower respiratory tract and a resultant oxidative stress.
- Rosen SH, Castleman B, Liebow AA: Pulmonary alveolar proteinosis. N Engl J Med 1958, 258:1123–1143.View ArticlePubMedGoogle Scholar
- Seymour JF, Presneill JJ: Pulmonary alveolar proteinosis. Progress in the first 44 years. Am J Respir Crit Care Med 2002, 166:215–235.View ArticlePubMedGoogle Scholar
- Singh G, Katyal SL, Bedrossian CW, Rogers RM: Pulmonary alveolar proteinosis: staining for surfactant apoprotein in alveolar proteinosis and in conditions simulating it. Chest 1983, 83:82–86.View ArticlePubMedGoogle Scholar
- Ramirez J, Harlan WR Jr: Pulmonary alveolar proteinosis. Nature and origin of alveolar lipid. Am J Med 1968, 45:502–512.View ArticlePubMedGoogle Scholar
- Harris JO: Pulmonary alveolar proteinosis: abnormal in vitro function of alveolar macrophages. Chest 1979, 76:156–159.View ArticlePubMedGoogle Scholar
- Carraway MS, Ghio AJ, Carter JD, Piantadosi CA: Detection of granulocyte-macrophage colony-stimulating factor in patients with pulmonary alveolar proteinosis. Am J Respir Crit Care Med 2000, 161:1294–1299.View ArticlePubMedGoogle Scholar
- Wang BM, Stern EJ, Schmidt RA, Pierson DJ: Diagnosing pulmonary alveolar proteinosis. A review and an update. Chest 1997, 111:460–466.View ArticlePubMedGoogle Scholar
- Shah PL, Hansell D, Lawson PR, Reid KBM, Morgan C: Pulmonary alveolar proteinosis: clinical aspects and current concepts on pathogenesis. Thorax 2000, 55:67–77.View ArticlePubMedPubMed CentralGoogle Scholar
- Trapnell BC, Whitsee JA, Nakata K: Pulmonary alveolar proteinosis. N Engl J Med 2003, 349:2527–2539.View ArticlePubMedGoogle Scholar
- Matulionis DH, Kimmel E, Diamond L: Morphologic and physiologic response of lungs to steroid and cigarette smoke: an animal model. Environ Res 1985, 36:298–313.View ArticlePubMedGoogle Scholar
- Witty LA, Tapson VF, Piantadosi CA: Isolation of mycobacteria in patients with pulmonary alveolar proteinosis. Medicine 1994, 73:103–109.View ArticlePubMedGoogle Scholar
- Ghio AJ, Jaskot RH, Hatch GE: Lung injury after silica instillation is associated with an accumulation of iron in rats. Am J Physiol 1994, 267:L686-L692.PubMedGoogle Scholar
- Weinberg ED: Iron and infection. Microbiol Rev 1978, 42:45–66.PubMedPubMed CentralGoogle Scholar
- Ghio AJ, Bassett M, Chall AN, Levin DG, Bromberg PA: Bronchoscopy in healthy volunteers. J Bronchol 1998, 5:185–194.View ArticleGoogle Scholar
- Turi JL, Yang F, Garrick MD, Piantadosi CA, Ghio AJ: The iron cycle and oxidative stress in the lung. Free Radic Biol Med 2004, 36:850–7.View ArticlePubMedGoogle Scholar
- Ghio AJ, Carter JD, Samet JM, Quay J, Wortman IA, Richards JH, Kennedy TP, Devlin RB: Post-transcriptional regulation of ferritin expression after in vitro exposures of human alveolar macrophages to silica is iron-dependent. Am J Respir Cell Mol Biol 1997, 17:533–540.View ArticlePubMedGoogle Scholar
- Ghio AJ, Carter JD, Richards JH, Crissman KM, Bobb HH, Yang F: Diminished injury in hypotransferrinemic mice after exposure to a metal-rich particle. Am J Physiol 2000, 278:L1051–1061.Google Scholar
- Kutnink MA, Skala JH, Sauberlich HE, Omaye ST: Simultaneous determination of ascorbic acid, isoascorbic acid (erythorbic acid), and uric acid in human plasma by high performance liquid chromatography with amperometric detection. J Liq Chromatogr 1985, 8:31–46.View ArticleGoogle Scholar
- Anderson ME: Determination of glutathione and glutathione disulfide in biological samples. Methods Enzymol 1985, 113:548–555.View ArticlePubMedGoogle Scholar
- Gutteridge JM, Mumby S, Quinlan GJ, Chung KF, Evans TW: Pro-oxidant iron is present in human pulmonary epithelial lining fluid: implications for oxidative stress in the lung. Biochem Biophys Res Comm 1996, 220:1024–1027.View ArticlePubMedGoogle Scholar
- Ghio AJ, Carter JD, Richards JH, Brighton LE, Lay JC, Devlin RB: Disruption of normal iron homeostasis after bronchial instillation of an iron-containing particle. Am J Physiol 1998, 274:L396-L403.PubMedGoogle Scholar
- Yang F, Friedrichs WE, Coalson JJ: Regulations of transferrin gene expression during lung development and injury. Am J Physiol 1997, 273:L417-L426.PubMedGoogle Scholar
- Pacht ER, Davis WB: Role of transferrin and ceruloplasmin in antioxidant activity of lung epithelial lining fluid. J Appl Physiol 1988, 64:2092–2099.View ArticlePubMedGoogle Scholar
- Van Snick JL, Markowetz B, Masson PL: The ingestion and digestion of human lactoferrin by mouse peritoneal macrophages and the transfer of its iron into ferritin. J Exp Med 1977, 146:817–827.View ArticlePubMedGoogle Scholar
- Cozzi A, Santambrogio P, Levi S, Arosio P: Iron detoxifying activity of ferritin. Effects of H and L human apoferritins on lipid peroxidation in vitro. FEBS Lett 1990, 277:119–22.View ArticlePubMedGoogle Scholar
- Balla G, Jacob HS, Balla J, Rosenberg M, Nath K, Apple F, Eaton JW, Vercellotti GM: Ferritin: a cytoprotective antioxidant strategem of endothelium. J Biol Chem 1992, 267:18148–53.PubMedGoogle Scholar
- Bell DY, Hook GE: Pulmonary alveolar proteinosis: analysis of airway and alveolar proteins. Am Rev Respir Dis 1979, 119:979–990.PubMedGoogle Scholar
- Hallman M, Sarnesto A, Bry K: Interaction of transferrin saturated with iron with lung surfactant in respiratory failure. J Appl Physiol 1994, 77:757–66.PubMedGoogle Scholar
- Ghio AJ, Hatch GE: Lavage phospholipids concentration after silica instillation in the rat is associated with complexed [Fe3+] on the dust surface. Am J Respir Cell Mol Biol 1993, 8:403–407.View ArticlePubMedGoogle Scholar
- Governa M, Valentino M, Tosi P, Luzi P, Miracco C, Sartorelli E, Loi F, Franzinelli A, Gori R: Pulmonary alveolar lipoproteinosis in rats following intratracheal injection of pyrite particles. J Toxicol Environ Health 1986, 19:403–12.View ArticlePubMedGoogle Scholar
- Balis JU, Paterson JF, Haller EM, Shelley SA, Montgomery MR: Ozone-induced lamellar body responses in a rat model for alveolar injury and repair. Am J Pathol 1988, 132:330–344.PubMedPubMed CentralGoogle Scholar
- Blank ML, Dalbey W, Nettesheim P, Price J, Creasia D, Snyder F: Sequential changes in phospholipid composition and synthesis in lungs exposed to nitrogen dioxide. Am Rev Respir Dis 1978, 117:273–280.PubMedGoogle Scholar
- Aso Y, Yoneda K, Kikkawa Y: Morphologic and biochemical pulmonary changes induced by bleomycin in mice. Lab Invest 1976, 35:558–568.PubMedGoogle Scholar
- Hayes JA, Snider GL, Wyatt : The evolution of biochemical damage in the rat lung after acute cadmium exposure. Am Rev Respir Dis 1976, 113:121–130.PubMedGoogle Scholar
- Hirano S, Kodama N, Shibata K, Suzuki KT: Distribution, localization and pulmonary effects of yttrium chloride following intratracheal instillation into the rat. Toxicol Appl Pharmacol 1990, 104:301–311.View ArticlePubMedGoogle Scholar
- Ghio AJ, Pritchard RJ, Dittrich K, Samet JM: Non-heme [Fe3+] in the lung increases with age in both the rat and man. J Lab Clin Med 1997, 129:53–61.View ArticlePubMedGoogle Scholar
- Thompson AB, Bohling T, Heires A, Linder J, Rennard SI: Lower respiratory tract iron burden is increased in association with cigarette smoking. J Lab Clin Med 1991, 117:493–499.PubMedGoogle Scholar
- Nelson ME, O'Brien-Ladner AR, Wesselius LJ: Regional variation in iron and iron-binding proteins within the lungs of smokers. Am J Respir Crit Care Med 1996, 153:1353–1358.View ArticlePubMedGoogle Scholar
- Ghio AJ, Carter JD, Richards JH, Richer LD, Grissom CK, Elstad MR: Iron and iron-related proteins in the lower respiratory tract of patients with acute respiratory distress syndrome. Crit Care Med 2003, 31:395–400.View ArticlePubMedGoogle Scholar
- Ghio AJ, Stonehuerner J, Richards J, Devlin RB: Iron homeostasis in the lung following asbestos exposure. Antioxidants & Redox Signaling 2007, in press.Google Scholar
- Pugh C, Hathwar V, Richards JH, Stonehuerner J, Ghio AJ: Disruption of iron homeostasis in the lungs of transplant patients. J Heart Lung Transplant 2005, 24:1821–1827.View ArticlePubMedGoogle Scholar
- Howe D, Mallavia LP: Coxiella burnetii infection increases transferring receptors on J774A.1 cells. Infect Immun 1999, 67:3236–3241.PubMedPubMed CentralGoogle Scholar
- Kasvosve I, Gomo ZA, Nathoo KJ, Matibe P, Mudenge B, Loyevsky M, Nekhai S, Gordeuk VR: Association of serum transferrin receptor concentration with markers of inflammation in Zimbabwean children. Clin Chim Acta 2006, 371:130–6.View ArticlePubMedGoogle Scholar
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