Figure 2

Cldn6 was immunolocalized during periods of murine lung morphogenesis. Cldn6 was initially detected in primitive respiratory epithelium at E12.5 (A) and expression persisted in differentiating epithelial cells at E13.5 (B), E14.4 (C), E15.5 (D), E16.5 (E), and E17.5 (F); however, expression diminished as development proceeded. Cldn6 immunoflorescence was revealed by Alexa Fluor® 488 secondary antibodies and DAPI staining was performed for cellular perspective. No immunoreactivity was observed in lung sections incubated without primary antibodies (not shown) and all images are at 40X original magnification. Scale bars represent 20 μm.