Alcohol ingestion disrupts alveolar epithelial barrier function by activation of macrophage-derived transforming growth factor beta1
© Curry-McCoy et al.; licensee BioMed Central Ltd. 2013
Received: 30 October 2012
Accepted: 12 March 2013
Published: 2 April 2013
Chronic alcohol abuse causes oxidative stress and impairs alveolar epithelial barrier integrity, thereby rendering the lung susceptible to acute edematous injury. Experimentally, alcohol-induced oxidative stress increases the expression of transforming growth factor β1 (TGFβ1) in the lung; however, we do not know the precise contribution of various alveolar cells in this process. In the present study, we focused on cell-cell interactions between alveolar macrophages and epithelial cells and the potential mechanisms by which TGFβ1 may become activated in the alveolar space of the alcoholic lung.
Primary alveolar macrophages and epithelial cells were isolated from control- and alcohol-fed Sprague–Dawley rats. Expression of TGFβ1 and the epithelial integrin αvβ6 were examined by real time PCR and either immunocytochemistry or flow cytometry. Alveolar epithelial cells were cultured on transwell supports in the presence of macrophage cell lysate from control- or alcohol-fed rats or in the presence of viable macrophages ± alcohol. Epithelial barrier function was assessed by transepithelial resistance (TER) and paracellular flux of Texas Red dextran.
TGFβ1 expression was increased in alveolar macrophages from alcohol-fed rats, and TGFβ1 protein was predominantly membrane-bound. Importantly, alveolar macrophage cellular lysate from alcohol-fed rats decreased TER and increased paracellular dextran flux in primary alveolar epithelial cell monolayers as compared to the lysates from control-fed rats. Alcohol-induced epithelial barrier dysfunction was prevented by anti-TGFβ1 antibody treatment, indicating the presence of bioactive TGFβ1 in the macrophage lysate. In addition, co-culturing macrophages and epithelial cells in the presence of alcohol decreased epithelial barrier function, which also was prevented by anti-TGFβ1 and anti-αvβ6 treatment. In parallel, chronic alcohol ingestion in vivo, or direct treatment with active TGFβ1 in vitro, increased the expression of αvβ6 integrin, which is known to activate TGFβ1, in alveolar epithelial cells.
Taken together, these data suggest that interactions between alveolar epithelial cells and macrophages contribute to the alcohol-mediated disruption of epithelial barrier function via the expression and activation of TGFβ1 at points of cell-cell contact.
Alcohol abuse impairs pulmonary innate immunity and renders individuals susceptible to pneumonia and lung injury . One of the important features of lung injury is disruption of alveolar epithelial barrier function . We have previously shown that chronic alcohol ingestion in rats increases oxidative stress  and transforming growth factor 1 (TGFβ1) expression in the lung , and inflammatory insults such as sepsis release activated TGFβ1 into the alveolar space, which can intensify lung injury by further disrupting alveolar barrier function. Ethanol ingestion decreases antioxidant glutathione in the alveolar space [5, 6], and TGFβ1 is a potent inhibitor of glutathione synthesis in lung epithelial cells . We have previously shown that ethanol ingestion also decreases another antioxidant, micronutrient zinc, in the lower airway  and adversely affects zinc importers through down-regulation of Kruppel-like transcription factor 4 by active TGFβ1 [9, 10]. Thus, TGFβ1 alters these important antioxidants in the lung and impairs epithelial barrier integrity. In addition, TGFβ1 is immunosuppressant [11, 12], and decreases immune function of alveolar macrophages by dampening GM-CSF receptors on these cells .
TGFβ1 protein has diverse and often contradicting biological activities regulating cell proliferation, differentiation, and function. TGFβ1 is synthesized as a latent complex and is present at the cell surface as latency-associated peptide (LAP) [14, 15]. LAP is non-covalently linked to TGFβ and prevents binding of active TGFβ1 to its receptors. The cell-associated TGFβ1 is activated by a variety of stimuli like plasmin or thrombospondin. In addition, TGFβ1 can be activated by integrin receptors on epithelial cells. Integrins are heterodimers composed of α and β subunits and mediate cell interactions with other cells and with the extracellular matrix. The integrin αvβ6, initially identified as fibronectin receptor, is predominantly expressed by the epithelium lining of airways and alveoli  and is up regulated in response to injury. It is one of the LAP binding receptors, which binds to LAP resulting in conformational changes in LAP and exposes TGFβ1 residing within LAP to the TGFβ1 receptors on the adjacent cells. This initial binding of TGFβ1 to its receptor is a key step in TGFβ1 signaling and its multiple effects on various cells.
Cells often use signaling molecules such as hormones, cytokines, growth factors, and chemotactic factors to transmit signals and communicate with other cells. In endocrine system hormones, secreted by cells at one site, travel and bind to a receptor on a cell at a distant site. Within any organ, including the lung, signaling molecules such as cytokines/growth factors are released in the local environment and bind to signal-transducing receptor on the adjacent cell (paracrine signaling) or bind to receptors on itself (autocrine signaling), thus activating cells. In juxtacrine signaling, cytokine/growth factor is membrane-bound and activates adjacent cells after binding to receptors . Thus, cell-cell communication in the alveolar compartment takes place in a paracrine, autocrine, or juxtacrine manner rather than in an endocrine manner. These types of cellular communications are important when examining the in vivo effects of a toxicant such as alcohol on the lung function.
The present study focuses on cell-cell interactions between alveolar macrophages and epithelial cells and extends our previous findings regarding TGFβ1’s role in disrupting epithelial barrier function. We evaluated the contribution of alveolar macrophages in alcohol-mediated impairment of alveolar epithelial barrier function. We report here that chronic alcohol ingestion increased TGFβ1 expression on alveolar macrophages. In parallel, alcohol ingestion increased integrin αvβ6 expression on epithelial cells. Importantly, co-culture of these cells in the presence of alcohol or culturing epithelial cells with macrophage lysates from alcohol-fed animals disrupted epithelial barrier function in a TGFβ1-dependent manner.
Materials and methods
Animals and alcohol feeding
Adult Male Sprague–Dawley rats (initial weights 150–200 g; Charles River Laboratory, Wilmington, MA) were fed the Lieber-DeCarli liquid diet (Research Diets, New Brunswick, NJ) containing either alcohol (ethanol; 36% of total calories) or an isocaloric substitution with maltose-dextrin ad lib for 6 weeks as previously published [17, 18]. All work was performed with the approval of the Institutional Use and Care of Animals Committee at the Emory University.
Brochoalveolar lavage and isolation of alveolar macrophages
Rats were anesthetized with 0.8 ml Euthasol containing penotobarbital sodium and phenytoin sodium (Vibac AH Inc, Fort Worth, TX). After pulmonary arterial perfusion, bronchial lavage was performed using 10mls of PBS 4 times and fluid was centrifuged at 405 g for 7 min to obtain alveolar macrophages. Cells were re-suspended in sterile F12-K complete medium containing antibiotics and 10% FBS for functional studies. This procedure routinely yields cells that are >98% viable by Trypan blue exclusion test .
Isolation of primary alveolar type II epithelial cells
Alveolar epithelial cells from control- and alcohol-fed rats were isolated using our established protocol . Briefly, lungs and trachea were removed as one unit and flushed with 40 ml of solution containing 16 mg of elastase. Lung lobes were cut and minced in a solution containing DNase I and newborn calf serum. The lung tissue suspension was shaken at 37°C for 10 minutes and filtered through 100 μm and 20 μm nylon mesh. The filtered lung suspension was then centrifuged at 405 g for 7 minutes, resuspended in 30 ml of complete medium containing DMEM/F12, antibiotics and fungicide, and plated on IgG coated dishes. Cells were incubated at 37°C 5% CO2 for 1 hour, and non-adherent cells were gently removed. Non-adherent type II cells were resuspended in complete medium and counted using a hemocytometer. Cell viability as determined by Trypan blue exclusion test was always >96%.
Alveolar epithelial barrier function
Epithelial barrier function was examined by measuring transepithelial electrical resistance (TER) and determining paracellular permeability to Texas Red dextran (Invitrogen). Rat alveolar type II epithelial cells were plated at 50,000 per well in a 24 wells transwell plate. Cells were cultured in DMEM/F12 complete medium and treated the next day with alcohol (60 mM), anti-TGFβ1 Ab (1 μg/ml), anti-αvβ6 Abs (1 μg/ml), or IgG. Alcohol, antibodies or IgG were added with the replacement of fresh medium every other day to all the transwells. Transepithelial resistance was measured after 6 days using an epithelial voltohmmeter (World Precision Instruments, Sarasota, FL) as described before . For paracellular permeability, sample transwells were placed in a plate containing 1 ml of 0.25 M-MgCl, 0.1 M-CaCl, PBS solution. Texas Red dextran (0.1 mg/ml) solution was added to the apical side of the monolayers in the transwell and basolateral samples were taken after 2 h incubation. The intensity of the dye was measured in a plate reader.
In some experiments, rat lung epithelial cell line L2 (ATCC CCL-149, Manassas, VA) and rat alveolar macrophage cell line NR8383 (ATCC CCL-2192) were used. Cells were cultured in F12K with 10% FBS and an antibiotic-antimycotic reagent (Sigma-Aldrich, St Louis, MO) at 37°C in 5% CO2. No TGFβ1 was detected in this culture medium containing FBS as measured by ELISA.
RNA isolation and Real-time PCR
RNA was extracted from cells using Qiagen RNeasy Mini Kit (Valencia, CA). Reverse transcription was performed using 1 μg RNA using iScript cDNA synthesis kit from Bio Rad (Hercules, CA), and real time polymerase chain reaction was performed using primers for rat TGFβ1 (5′-TGAGTGGCTG-TCTTTTGACG-3′ and 5′-TGGGACTGATCCCATTGATT-3′), rat integrin chains αv (5′-GGGCATTTCAGGACTTGTGT-3′ and 5′-AGGTGACGGGACTCAAC-AAC-3′) & β6 (5′-AGGCCTGCTCTGTGGAGATA-3′ and 5′-CCATCTGC-AGACAGGTAGCA-3′) that were designed in our laboratory and obtained from Invitrogen (Carlsbad, CA). 18S Quantum RNA classic II primers were purchased from Ambion (Austin, TX). All samples were run in triplicate. Messenger RNA expression for each gene of interest was normalized to 18S housekeeping gene and then expressed as the change relative to the control group.
Flow cytometric analysis
Flow cytometric analysis of membrane protein expression was performed using an established protocol in our laboratory . Briefly, cells were not permeabilized and incubated with a primary polyclonal antibody or IgG (Santa Cruz biotechnology, Santa Cruz, CA) for one hour. Cells were washed with PBS, and stained with a PE-conjugated secondary antibody. The labelled cells were washed again with PBS and analyzed by FACScan flow cytometer (BD Bioscience, San Diego, CA). Data are expressed as percentage of cells positive for the protein.
To a portion of the stained macrophages from the above protocol, Hoechst nuclear stain (Molecular Probes, Eugene, OR) was added. Cells were washed and put on slides to obtain images using a microscope equipped with epifluorescence (Olympus Corporation, Center Valley, PA).
TGFβ1 protein in the bronchoalveolar lavage and cells was measured using ELISA kit (BD biosciences).
Data are presented as mean ± SEM. Data analysis was performed by unpaired t test for two treatment groups and ANOVA with Student-Newman-Keuls test for group comparison for three or more treatment groups and was considered statistically significant at a value of p < 0.05.
Chronic alcohol ingestion increased expression of TGFβ1 in alveolar macrophages
Bioactive TGFβ1 was present in the alveolar macrophage lysates from alcohol-fed rats
Alcohol exposure decreased barrier function in alveolar epithelial cells co-cultured with alveolar macrophages via a TGFβ1-dependent mechanism
The effect of alcohol on the expression of integrin chains αvβ6 on alveolar epithelial cells
We previously reported that chronic alcohol ingestion in an experimental rat model increased TGFβ1 and that this is associated with alcohol-mediated epithelial dysfunction . Further, although there was no evidence of TGFβ1 release into the alveolar space during baseline or ‘unstressed’ conditions, there was a marked increase in the activation and release of TGFβ1 into the alveolar space in response to acute endotoxemia, further enhancing alveolar epithelial barrier disruption . These experimental findings suggested that this could be a contributing mechanism underlying the strong association between alcohol abuse and an increased risk of acute lung injury . In the present study, although we again determined that TGFβ1 is not activated and released into the extracellular fluid spontaneously, it is activated in situ in the non-septic alcoholic lung. This mechanism could explain why even in the absence of an acute stress such as sepsis there is nevertheless alveolar epithelial barrier dysfunction even in the otherwise healthy experimental animals during chronic alcohol ingestion as reflected by increased paracellular leak of radiolabeled albumin . We show here that (1) alveolar macrophages from alcohol-fed rats have increased expression of TGFβ1 and that the TGFβ1protein is membrane-bound, (2) co-culture of alcohol-primed macrophages and epithelial cells disrupted alveolar epithelial barrier function in a αvβ6- and TGFβ-dependent manner, (3) treating alveolar epithelial cells with the lysates of alveolar macrophages from alcohol-fed rats decreased their barrier function and this effect was antagonized by co-treating with an anti-TGFβ1 antibody, (4) alveolar epithelial cells from alcohol-fed rats had increased expression of the integrin chains αv and β6, and (5) treating alveolar epithelial cells with active TGFβ1 in vitro also increased the expression of these integrin chains, suggesting a forward feedback in which TGFβ1 induces the expression of the integrin that activates it.
The lung is comprised of multiple cell types including alveolar epithelial cells and macrophages in close vicinity, and cells communicate with each other, either in a paracrine manner through locally secreted cytokines/growth factors, or in a juxtacrine manner via cell-associated cytokines or growth factors. We identified membrane-bound TGFβ1 on alveolar macrophages suggesting a juxtacrine interaction with adjacent epithelial cells. Whether TGβ1 is stored in the extracellular matrix or on the surface of alveolar macrophages, it is present as a latent complex within a prodomain that shields it from binding to its receptors. The binding of the αv chain to an RGD sequence in the prodomain and exertion of force on this domain changes its conformation and activates TGFβ1, which can then bind to TGFβ receptors and initiate a wide range of intracellular signals.
Cell surface integrins regulate cell growth, migration, and survival. The αvβ6 integrin is a transmembrane glycoprotein that is mainly expressed by injured epithelium . Integrins participate in activation of growth factors and initiate intracellular signaling cascades in response to receptor binding . The integrin αvβ6 binds to the latency-associated peptide leading to activation of TGFβ1 . Studies in αvβ6 knockout mice showed a deficiency in TGFβ1 activation by the epithelium and increased inflammation in response to injury and infection. Further, transgenic mice with a targeted deletion of the β6-integrin developed exaggerated lung inflammation  that was prevented by restoring β6 expression. Interestingly, bleomycin treatment leads to lung fibrosis due to increased activation of TGFβ1 . In vivo, the αvβ6 integrin is an activator of TGFβ1, which stimulates fibroblast proliferation and collagen production and has been implicated in fibrosis .
TGFβ1 is known to regulate many biological processes. Cells produce TGFβ1 as a latent complex and the active peptide must be released from this complex in order to be activated and bind its receptors. Activation of TGFβ1 within the epithelium by the αvβ6 integrin plays a role in many diseases , including airway hyperresponsiveness in allergic asthma . Other integrins such as αvβ5 are implicated in TGFβ1 activation in myofibroblast differentiation in fibrotic lungs . In contrast to stress fibers used by fibroblasts and other contractile cells, epithelial cells exert force on latent TGFβ using actin/myosin . Nevertheless, in both cell types mechanotransducers are involved in TGFβ1 activation. In idiopathic pulmonary fibrosis, the lung epithelium plays a key role in the fibrotic response and integrin-mediated activation of TGFβ1 has been implicated as a primary driver of this pathophysiology . In fact, the activation of TGFβ1 by αvβ6 has been proposed as a potential therapeutic target for fibrotic lung diseases. In experimental model of airway fibrosis Mitchell et al. showed that chronic alcohol ingestion was associated with amplification of airway fibrosis through increase in IL-13 signalling . Interestingly, IL-13 modulates TGFβ1 signalling during airway fibrosis, and alcohol’s priming effect for increased IL-13 signalling may play a role in lung transplantation related injury. Integrin αvβ6 plays a role in acute lung injury induced by Pseudomonas aeruginosa and deletion of this integrin provides protection in experimental models of lung injury due to bleomycin or high tidal volume ventilation. Lung biopsies from patients with a diagnosis of IPF show staining for integrin αvβ6 within pneumocytes , and partial inhibition of TGFβ using integrin αvβ6 antibodies was effective in blocking murine pulmonary fibrosis without inducing an inflammatory response. Beta 6 integrin expression increased within the alveolar epithelium in radiation induced fibrosis model  and anti-αvβ6 therapy prevented fibrosis. Many αv integrins play a role in preventing inappropriate vascular growth and controlling vascular permeability, and studies in mice lacking the beta 6 subunit found a role for integrin-mediated TGFβ1 activation in pulmonary and renal fibrosis, acute lung injury, and pulmonary emphysema . These studies elucidate the important potential contributions of αvβ6-mediated activation of TGFβ1 in many diseases.
Cytokines such as TGFβ1 have multiple and often diverse functions on different cell types. Activated TGFβ1 in situ can disrupt epithelial cell function and barrier integrity causing edema. In addition, active TGFβ1 can favor proliferation of fibroblasts and this has potential to lead to fibrosis or can decrease immune function of macrophage . Thus, alcohol’s damaging effects on the lung involve multiple cell types and interactions between them. One of the mechanisms by which chronic alcohol abuse leads to oxidative stress includes activation of renin-angiotensin system in the lung. Alcohol-induced amplification of the renin-angiotensin system appears to be the major cause of the alveolar epithelial oxidant stress and TGFβ1-mediated barrier disruption . Examining cell-cell interactions in the lung such as done in the current study may discover, in future, interconnected pathophysiological targets for alcohol abuse.
Authors would like to thank Robert Raynor and S. Todd Mills for technical assistance.
Supported by NIAAA (T32AA013528), P50 AA013528, RO1AA017627.
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