Fig. 6

Ectopic expression of HMGCS2 in mice AECIIs mitigated Bleomycin induced lung fibrosis and lipid accumulation. A, Scheme of the establishment of mice with AECIIs overexpressing HMGCS2, wild-type C57BL/6 J mice were administered HBAAV2/6-SPC-T2A-ZsGreen and HBAAV2/6-SPC-mHMGCS2-T2A-ZsGreen virus and Bleomycin was intratracheally delivered 3 weeks later to establish lung fibrosis. B,C, Body weight curve and hydroxproline content after Bleomycin treatment. D, HMGCS2 expression and Collagen1 content were determined by western blot. E, Masson’s Trichrome and Oil red staining of lung sections to visualize collagen deposition and lipid accumulation, scale bar = 50 μm. Data are expressed as mean ± SD, *: p < 0.05; **: p < 0.01