Fig. 2
From: PI3Kγδ inhibition suppresses key disease features in a rat model of asthma
Activation of the PI3K, JAK/STAT and NF-κB pathways in lung is associated with increased cellular inflammation and the late asthmatic response (LAR). Akt phosphorylation, a downstream marker of PI3K pathway activation, was assessed after antigen challenge (OVA) in whole lung homogenate (A and B) pSTAT3 (C), pSTAT5a, b (D) and NF-κB (E) levels were assessed in whole lung homogenate and nuclear extract, respectively. Bronchoalveolar lavage (BAL) was taken to assess total leukocytes (F), neutrophils (G) & eosinophils (H) numbers. Whole body plethysmography in unrestrained rats was used to monitor the late asthmatic response (LAR; I). Data are presented as boxes (Q2 to Q3 with the median) and whiskers (min to max). Statistically significant differences between time matched, saline and OVA challenge groups is indicated with **p < 0.01 and ****P < 0.0001. Two experiments (n = 6 or 8)