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Fig. 5 | Respiratory Research

Fig. 5

From: Neutrophil extracellular traps promote proliferation of pulmonary smooth muscle cells mediated by CCDC25 in pulmonary arterial hypertension

Fig. 5

NETs affect PASMCs proliferation through CCDC25 in vitro. (A) CCK-8 assay to detect the effect of NETs and CCDC25 on PASMCs proliferation. (B-C) EdU to detect the effect of NETs and CCDC25 on PASMCs proliferation. (D-E) Flow cytometry to detect the effect of NETs and CCDC25 on PASMCs cell cycle. (F-G) Western blotting analysis of proliferation related PCNA in shCCDC25 PASMCs. Protein levels were normalized to β-actin. All results were presented as the mean ± SEM (n = 3). The p values were determined by Student’s t-test. NS: p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

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Respiratory Research

ISSN: 1465-993X

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