Fig. 2

NETs was involved in the development of PAH. (A-C) Concentrations of cf-DNA, MPO and NE in human peripheral blood serum. (D-F) Correlation between NETs concentration and mPAP. (G) CCK-8 assay to detect the effect of NETs on PASMCs proliferation. (H) EdU to detect the effect of NETs on PASMCs proliferation. (I) Flow cytometry to detect the effect of NETs on PASMCs cell cycle. (J) Western blotting analysis of proliferation related PCNA in PASMCs. Protein levels were normalized to β-actin. All results were presented as the mean ± SEM (n = 3). The p values were determined by Student’s t-test. NS: p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001