Fig. 4

LPS-induced transitioning CD11c^intCD11b^hi monocytes/macrophages are reduced with systemic delivery of clodronate liposomes. Mice were pre-treated with vehicle (Veh) or clodronate (Clod) liposomes 24 h prior to a one-time treatment with LPS (10 μg) or saline (Sal) control and euthanized at 48 h. Scatter plot with bars depicting mean with SD. A Total lung cells enumerated. B Representative contour plot of the four monocyte (mono)-Mɸ subpopulations across groups based upon CD11c and CD11b expression after removal of neutrophils gated from live CD45⁺ cells after excluding debris and doublets. C Number of CD11c⁺CD11b^lo alveolar (Alv) Mɸ, CD11c⁺CD11b^hi activated (Act) Mɸ, CD11c^intCD11b^hi mono-Mɸ, and CD11c⁻CD11b.^hi monocytes determined by multiplying lung cell % population by total lung cells enumerated from lung sample. (#p < 0.05 vs. respective saline) and (*p < 0.05 denoted by line with brackets denoting difference between groups). N = 8 (Veh + Sal), 8 (Clod + Sal), 8 (Veh + LPS), 9 (Clod + LPS)