Fig. 1

Inhalation of organic dust extract (ODE), lipopolysaccharide (LPS), and peptidoglycan (PGN) induce lung CCR2⁺ monocyte-macrophages (Mɸ) and monocytes. CCR2^RFP/+ mice were exposed once to ODE (25%), LPS (10 μg), PGN (100 μg), or saline control and euthanized at 48 h. Scatter plots with bars depict mean with SD delineating cells as total (gray), CCR2⁺ (green), and CCR2⁻ (red). A Total lung cells enumerated. B Representative contour plot of the four monocyte (mono)-Mɸ subpopulations across groups based upon CD11c and CD11b expression after removal of neutrophils gated from live CD45⁺ cells after excluding debris and doublets. C RFP ± staining by exposure group and subpopulation. D CD11c⁺CD11b^lo alveolar (Alv) Mɸ, CD11c⁺CD11b^hi activated (Act) Mɸ, CD11c^intCD11b^hi mono-Mɸ, and CD11c⁻CD11b.^hi monocytes determined by multiplying lung cell % population by total lung cells enumerated from lung sample. Statistical analyses were performed with Kruskal–Wallis with Dunn’s test for multiple comparisons (#p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001) vs. respective saline. N = 19 (saline), 9 (ODE), 8 (LPS), 5 (PGN)