Fig. 7
From: Use of non-small cell lung cancer multicellular tumor spheroids to study the impact of chemotherapy
CaPa and CaGe treatment effect on complex MCTS. Lung cancer cells, H1975 or H1437, fibroblasts (HFF-2) and monocytes were grown as MCTS. MCTS were treated with a combination of carboplatin 50µM and paclitaxel 150nM (CaPa) or gemcitabine 100nM (CaGe) repeated 3 times. (A) Cells viability was measured. (B) mRNA from cells were extracted and CD274 expression was measured using real-time PCR. Results are expressed as the mean +/- SEM. n = 5. *, p < 0.05 (Mann-Whitney t-test)