Fig. 5

Impact of carboplatin-paclitaxel treatment on complex MCTS viability and structure. Lung cancer cells, ADCA117, fibroblasts (HFF-2) and monocytes were cultured as MCTS. MCTS were treated with a combination of carboplatin 50µM and paclitaxel 150nM (CaPa) or gemcitabine 100nM (CaGe) repeated 3 times. (A) MCTS were observed using optical microscopy (upper panel) or histology using hematoxylin, phloxin, safran staining (lower panel) and (B) cell viability was measured. Results are expressed as the mean +/- SEM of three independent experiments. ***, p < 0.001 (Mann-Whitney t-test). (C) MCTS were observed using confocal microscopy. Blue: nuclei, green: ADCA117-GFP and red: HFF-2 ruby. (D) MCTS were dissociated and analyzed using flow cytometry. Dot plot shows CD14 and CD163 expression on HLA-DR + and CD14 + cells. (E) mRNA were extracted and both CD14 and CD163 expressions were measured using real-time PCR. Results are expressed as the mean +/- SEM of the three independent experiments