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Fig. 1 | Respiratory Research

Fig. 1

From: A WNT mimetic with broad spectrum FZD-specificity decreases fibrosis and improves function in a pulmonary damage model

Fig. 1

(A) Strategy to start AT2 organoid outgrowth from a purified primary cell population. (B) Gene expression profile of epithelial lung cell type markers in a population of purified cells used for organoid culture. (C) Representative image of mouse AT2 protein expression. Scale bar, 20 μm. (D) Mouse AT2 organoids expanded with CHIR99021 or L-F12578 + RSPO2 as a Wnt source. (E) Outgrowth efficiency assay of mouse AT2 organoids with various treatments. Statistical analysis: one-way ANOVA, displayed p-value is comparison to Base + C59. **** P value ≤ 0.0001, *** ≤ 0.001, ** ≤ 0.01. (F) Representative images of two treatment conditions. (G) Representative images of human AT2 organoid morphology and protein expression level after expansion. (H) Outgrowth efficiency assay of human AT2 organoids. Wnt mimetic concentration, 5 nM; RSPO2 concentration, 20 nM. (I) Representative images at day 12

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Respiratory Research

ISSN: 1465-993X

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