Fig. 4

GLUT3 played a crucial role in airway remodelling in experimental COPD (A) The Schematic chart of Male C57BL6J mice treatment (B) The efficiency of AAV infection in mouse lung tissues is shown in immunofluorescence images, scale bars, 50 μm. (C-D) Western blot showed the expression of GLUT3 and GAPDH in the lungs of mice exposed to air and CS (n = 3 per group). (E) Pulmonary function was represented as Penh (n = 3 per group) (F) Performing Masson staining on lung tissue assessed lung morphology. Nuclei: black; collagen: blue; cytoplasm/epithelial: red. Scale bars, 200 μm. (G) Quantitative staining of Masson content in mice (n = 3 per group). (H-L) Western blot showed ZEB1, E-cadherin, N-cadherin, and Vimentin in the lungs of mice (n = 3 per group). The data are the mean ± SD. * P < 0.05, ** P < 0.01,*** P < 0.001