Fig. 3

GLUT3 increased in mice exposed to CS and BEAS-2B cells treated with CSE. (A) and (B) Western blot showed the expression levels of GLUT3 and GAPDH in the lungs of mice exposed to air (n = 3) and CS (n = 3). (C) and (D) Representative GLUT3 immunostaining (brown staining) was performed in the airways of mice exposed to air (n = 3) and CS (n = 3), Scale bars, 100 μm(up). (E) and (F) Western blot detected the time course of GLUT3 expression in the 5% CSE response. (G) Immunofluorescence results showed that after 48 h of treatment with 5% CSE in BEAS-2B cells, GLUT3 protein expression was enhanced, scale bar, 100 μm. The data are the mean ± SD. * P < 0.05, ** P < 0.01,*** P < 0.001