Fig. 2

Airway remodelling was triggered in CS-induced experimental COPD. (A) Schematic diagram of male C57BL6J mice exposed to air or CS treatment. (B) Conducting H&E staining on lung sections, scale bar, 100 μm (up). (C) Conducting Masson staining on lung sections assessed lung morphology. Nuclei: black; collagen: blue; cytoplasm/epithelial: red. Scale bars, 100 μm(up). (D) Quantitative staining of Masson content in control group (n = 3) mice and CS exposed group mice(n = 3). (E) The Penh content in CS-exposed mice (n = 5) increased compared to the control group (n = 5). (F-J) Western blot showed the ZEB1, E-cadherin, N-cadherin, and Vimentin in the lung tissue of CS-exposed mice and the control group (n = 3). The data are the mean ± SD. * P < 0.05, ** P < 0.01,*** P < 0.001