Fig. 1

GLUT3 expression was increased in airway epithelium, and airway remodelling occurred in patients with COPD (A) and (C) The heat maps of GSE20257 and GSE10006. (B) and (D) The box plots of GSE20257 and GSE10006. (E) Representative airway GLUT3 immunohistochemical staining on lung sections of Con-NS, nonsmokers without COPD (n = 5), Con-S, smokers without COPD (n = 5), and COPD, COPD patients (n = 5). Scale bar, 100 μm (up). (F) Quantitative analysis of GLUT3 protein levels in airway epithelial cells. (G) and (H) The relative protein level of GLUT3 in the lung sections of Con-NS (n = 5), Con-S (n = 5), and COPD (n = 5) were determined by Western blot. (I) Western blots (J) The relative protein level of ZEB1, E-cadherin, N-cadherin, and Vimentin in the lungs of Con-NS (n = 8), Con-S (n = 8), and COPD (n = 8). (K) Perform Masson staining on lung sections to evaluate lung morphology. Scale bar, 100 μm. Collagen: blue, nucleus: black, cytoplasm/epithelial cells: red. (L) Quantification of Masson staining for collagen content from Con-NS (n = 3), Con-S (n = 3), and COPD patients (n = 3). The data are the mean ± SD. * P < 0.05, ** P < 0.01,*** P < 0.001