Fig. 3
From: Loss of ZNF451 mediates fibroblast activation and promotes lung fibrosis
ZNF451 overexpression aggravates BLM-induced PF. A Schematic overview of the experimental design for (C-F). B Immunofluorescence staining of ZNF451 in lung tissue sections from Lenti-Ctrl- or Lenti-Znf451-treated mice. Scale bars, 50 μm. C Masson staining was performed to evaluate fibrotic changes in the indicated mice (Group 1: PBS + Lenti-Ctrl, n = 6; Group 2: PBS + Lenti-Znf451, n = 6; Group 3: BLM + Lenti-Ctrl, n = 7; Group 44: BLM + Lenti-Znf451; n = 6). Scale bars, 100 μm. D Cst was measured to evaluate the lung function of BLM-exposed mice after Znf451 overexpression. E Hydroxyproline content in lungs from BLM-exposed mice after Znf451 overexpression. F RT‒PCR of mRNA expression of fibrosis genes in lungs from BLM-exposed mice after Znf451 overexpression