Fig. 1

Expression of ZNF451 is decreased in PF lungs and is negatively associated with PF. A Scatter plots of ZNF451 expression in lung tissues of patients with IPF and normal controls from a public microarray dataset (GSE24206) (normal, n = 6; early IPF, n = 8; advanced IPF, n = 9). B Scatter plots of ZNF451 expression in lung tissues of patients with SSc-PF and normal controls from a public microarray dataset (GSE76808) (normal, n = 4; SSc-PF, n = 14). C RT‒PCR of ZNF451 mRNA in lung tissues of patients with PF and controls (control, n = 4; PF, n = 4). D Immunoblots of ZNF451 in lung tissues from PBS- or BLM-challenged mice (PBS mice, n = 4; BLM mice, n = 4). E Representative images showing ZNF451 IHC in lung tissues from control mice and BLM-induced PF mice. Scale bars, 50 μm. F RT‒PCR of Znf451 mRNA in lung tissues from PBS- or BLM-challenged mice (PBS mice, n = 6; BLM mice, n = 6). G Correlation analysis between Znf451 expression in the lungs and static compliance of the respirable system (Cst) in mice. Each point represents the value of one mouse. Spearman’s rank correlation test was employed to determine statistical significance (BLM, n = 15). H Immunoblots of ZNF451 in primary lung fibroblasts from PBS- or BLM-challenged mice (n = 4 per group)