Fig. 6

Nr2f2 overexpression attenuated bleomycin-induced mouse lung fibrosis. (A) The schematic diagram depicts the timeline for intratracheal instillation of AAV2/9, AAV2/9-Nr2f2, and bleomycin in a mouse model of bleomycin-induced pulmonary fibrosis. (B) Representative axial micro-CT images of the mouse lungs following 21 days of exposure to bleomycin. (C) Hydroxyproline content analysis was performed on the entire right lung of mice infected with AAV2/9 or AAV2/9-Nr2f2 treated with either saline or bleomycin. (D) qRT-PCR analysis the changes in gene expression of Nr2f2, Fn1, Col1a1, and Acta2 in lung tissues of AAV2/9 or AAV2/9-Nr2f2 infected mice treated with either saline or bleomycin. (E) WB analysis the changes in protein expression of Nr2f2, Fibronectin, Col1a1, and α-SMA in lung tissues of AAV2/9 or AAV2/9-Nr2f2 infected mice treated with either saline or bleomycin. (F) Representative photomicrographs of H&E-staining, Masson’s trichrome-staining, and Fibronectin, Col1a1, and α-SMA IHC staining on lung sections from each group of mice. Data are shown as the mean ± SD. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001