Fig. 1

Decreased NR2F2 expression in fibrotic lungs and bleomycin-induced lung epithelial cells. (A) The expression levels of p21 and p16 in lung tissues from mice treated with saline or bleomycin were quantified using WB analysis. (B) Representative images of SA-β-Gal staining in lung tissues from mice treated with saline or bleomycin. (C) Representative IHC staining images of NR2F2 in lung sections obtained from control subjects and IPF patients (n = 3). (D) The transcriptional change in Nr2f2 expression was analyzed using qRT-PCR in mice induced with saline or bleomycin. (E) Representative images of IHC staining using anti-NR2F2 on lung sections obtained from mice induced with saline or bleomycin. (F) The changes in protein levels of NR2F2, α-SMA, and Fibronectin were analyzed using WB in mice induced with saline or bleomycin. (G-I) WB analysis of NR2F2 and p21 protein expression in A549 cells (G), MLE-12 cells (H), and BEAS2B cells (I) treated with saline or bleomycin for 3 days. (J) The transcriptional changes in NR2F2 were analyzed using qRT-PCR in A549 cells, MLE-12 cells, and BEAS2B cells induced with saline or bleomycin for 3 days. (K) WB analysis was performed to examine the changes in NR2F2 protein expression in primary AECs isolated from mice treated with either saline or bleomycin. Data are shown as the mean ± SD. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001