Fig. 5
From: Bhlhe40 deficiency attenuates LPS-induced acute lung injury through preventing macrophage pyroptosis
Bhlhe40 deficiency suppresses LPS-induced inflammatory cytokine production in BMDMs and in RAW264.7 cell line. (A) Il-1β levels in the culture supernatant of BMDMs. (B) Il-1β levels in the culture supernatant of RAW264.7 cells. (C) Relative mRNA levels of Il-6, Tnf-α, Cxcl10 and iNOS in BMDMs were assessed by qRT-PCR. (D) Relative mRNA levels of Il-6, Tnf-α, Cxcl10 and iNOS in RAW264.7 cells were assessed by qRT-PCR. n = 3. Data are shown as the mean ± SEM. Statistical analysis was performed by two-way ANOVA followed by Bonferroni’s multiple comparisons test or unpaired two-tailed Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001