Fig. 3
From: Bhlhe40 deficiency attenuates LPS-induced acute lung injury through preventing macrophage pyroptosis
Bhlhe40 deficiency alleviates GSDMD-mediated pyroptosis and caspase-1 and caspase-11 pathways in LPS-induced ALI mice. (A) Representative Western blot of GSDMDFL and GSDMDNT in the lung tissues of mice. (B) Representative Western blot of cleaved IL-1β in the lung tissues of mice. (C) Representative images of F4/80 and GSDMDNT double-immunofluorescence staining of LPS-induced lungs. Scale bar = 50 μm and 20 μm. (D) Representative Western blot of pro-caspase-1, cleaved caspase-1, pro-caspase-11, cleaved caspase-11, NLRP3, ASC, TLR4 and MYD88 in the lung tissues of mice. n = 6