Fig. 1

Bhlhe40 is upregulated in lung tissues and macrophages in LPS-induced ALI. (A) Representative Hematoxylin-eosin staining (H&E) of lung tissues in mice stimulated by LPS for different times. Scale bar = 100 μm. (B) Lung injury score based on H&E staining. (C-I) Relative mRNA levels of inflammatory cytokines (Il-1β, Il-6, Tnf-α, Ifn-γ, Cxcl10, Mcp-1, Il-10) in the lungs of mice after LPS induction for different times. (J-L) qRT-PCR and Western blots and statistical analyses of Bhlhe40 in the lungs of mice after LPS induction for different times. (M) Representative images of immunohistochemistry for Bhlhe40 expression in the lungs of mice. Scale bar = 50 μm and 25 μm. (N) Representative images of F4/80 and Bhlhe40 double-immunofluorescence staining of LPS-induced lungs. Scale bar = 50 μm and 20 μm. n = 6. Data are shown as the mean ± SEM. Statistical analysis was performed by one-way ANOVA followed by Bonferroni’s multiple comparisons test or unpaired two-tailed Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001, ^nsp > 0.05