Fig. 3

Cellchat analysis illustrating cell-cell interaction specific to asthma. (A) Circle plot showing the number of ligand-receptor interactions between pairwise cell populations among the six major cell populations in NC and AS groups. The strength of ligand-receptor (L-R) interactions between cell population pairs was visualized with the number of L-R pairs labeled, and edge width was proportional to the number of L-R pairs. (B) The heatmap depicted the different number of pairwise cell interactions between AS and NC groups where top colored bar plot denoted the sum of incoming signaling and right colored bar plot represented the sum of outgoing signaling in each cell cluster. The color bars in red and blue respectively denoted an increase and decrease in the number of interactions in AS compared to NC group. (C) Wordcloud visualizing the up-regulated and down-regulated ligands in asthma. Word size indicates the extent of enrichment in asthma or control group. (D) Chord diagram illustrated ligand-receptor pairs and their weight contributing to the signaling from fibroblast subpopulations and macrophage subpopulations to the selected signaling receiving cells. (E) Comparison of the significant ligand-receptor pairs between AS and NC groups, which contribute to the signaling pathways from fibroblast subpopulations to the selected cells described in Fig D. Dot color represented communication probability and dot size denoted p-value. (F) Hierarchical plot deciphering cell communication network of CCL signaling in AS group. Left and right panels showed autocrine and paracrine signaling to selected signaling receiving cells and sender cells. The width of edge reflected the communication probability. (G). Circle plot showing inferred CCL8-CCR2 pair in NC (left) and AS group (right). (H). Violin plots displaying expression level of selected genes in major senders and receivers in NC (grey) and AS group (red)