Fig. 1

A 16-week model of HDM-induced asthma results in airway inflammation, airway remodeling and hyperresponsiveness. (A) Schematic diagram of established asthma model and subsequent scRNA-seq analysis. (B) Body weight of asthma group was markedly reduced. (C) Ratio of lung/body weight was significantly reduced in asthma group. (D) sRaw of asthma group was increased compared to controls. (E) The number of total cells, eosinophils, lymphocytes and neutrophils in BALF were compared between and asthma group and controls. (F) Comparison of H&E, PAS and Masson staining in mice lung section of asthma group and controls. (G) The expression level of α-SMA was significantly increased in lung tissues of asthma group. (H) Inflammation score in asthma group was significantly increased. (I) PAS score in asthma group was significantly increased compared with controls. (J) Masson’s score of asthma group was markedly increased. (K) Thickness of airway wall in asthma group was significantly increased compared to controls. (L) α-SMA positive areas of asthma group were remarkably increased. The values are shown as the mean ± SD (n = 6). ##P < 0.01 vs. and #P < 0.05. Scale bar, 50 μm. The values are shown as the mean ± SD (n = 6). ^***P < 0.001νs., ^**P < 0.01 νs. and ^*P < 0.05νs. Control group