Fig. 3
From: Airway disease decreases the therapeutic potential of epithelial stem cells
Identification of squamous basal cells. A Normal tracheal basal cell colonies under 10x magnification form discrete colonies with distinct edges. Representative immunofluorescence staining demonstrates high levels of K5 (green) and K14 (red), markers associated with airway epithelial stem cells, at low B and high C magnification. Representative double staining with epithelial cell markers CD151 (α-CD151-APC) and CD49f (α-CD49f-eFlour-450) in normal D and stenotic E regions. F Squamous metaplasia cells under 10x magnification exhibit a diffuse growth pattern and no discrete edges.Representative immunofluorescence staining demonstrates high levels of K5 (green) and K14 (red), markers associated with airway epithelial stem cells, at low H and high I magnification. Representative double staining with epithelial cell markers CD151 (α-CD151-APC) and CD49f (α-CD49f-eFlour-450) in normal I and stenotic J regions