Table 1 Parameters used in Qupath script
Parameters | Values |
|---|---|
Probability (detection) threshold | 0.5 |
Selected detection channel | DAPI |
Percentile normalization | 1, 99 |
Resolution for detection (pixel size) | 0.275 |
Cell expansion | 1.0 |
Cell constrains scale | 1.5 |
Probability | True |
Intensity threshold parameters (localization) | |
SP-C (cytosol) | 800 |
KRT17 (cytosol) | 400 |
KRT5 (cytosol) | 400 |
KRT14 (cytosol) | 700 |
p63 (nucleus) | 1000 |
AcTub (membrane) | 400 |
SCGB1A1 (cytosol) | 400 |
MUC5AC (cytosol) | 300 |
MUC5B (cytosol) | 300 |
MMP7 (cytosol) | 600 |