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Fig. 3 | Respiratory Research

Fig. 3

From: The use of cultured human alveolar basal cells to mimic honeycomb formation in idiopathic pulmonary fibrosis

Fig. 3

Alveolar basal cell marker expression is stable over several passages and after cryopreservation. Experiments presented in this figure were performed in alveolar BC from 4–5 different IPF patients (n-number). RNA expression levels (− ΔCt) of KRT5, TP63, CDH1 or CDH2 in alveolar BC (n = 5) at passage (p) 0, 2, 4, and 6 (A). Representative immunoblots (cropped) showing the protein expression of KRT5, p63, CDH1, CDH2 or GAPDH in alveolar BC (n = 4) at p1, p3, and p5 (B) and their densitometry analysis relative to the house keeping gene GAPDH. The corresponding uncropped immunoblots are provided in figure S1. Dots within the bar charts represent datapoints generated for alveolar BC from each patient (C). RNA expression levels (− ΔCt) of KRT5, TP63, CDH1 or CDH2 in alveolar BC (n = 5), before (fresh) and after (thawed) cryopreservation (D). Data are expressed as mean ± SEM (Mann–Whitney test, * indicates p < 0.05)

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Respiratory Research

ISSN: 1465-993X

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