Fig. 2

Characteristics of cultured alveolar basal cells. Illustration of alveolar BC culture (A). Experiments presented in this figure were performed in alveolar BC from 3 to 6 different IPF patients (n-number). RNA expression levels (− ΔCt) of TP63, KRT5, KRT14, KRT17, CDH2 and SCGB1A1 in alveolar BC (n = 6). Data are expressed as mean ± SEM (B). Representative single- or merged ICC/IF images of alveolar BC (n = 4) incubated with antibodies detecting KRT5, p63, KRT17, KRT14, SCGB1A1, or CDH2. Nuclei were counter-stained with DAPI. Automated cell counts of alveolar BC at 0 and 48 h (n = 4) (D). Alveolar BC wound closure between 0 and 24 h (n = 3) (E). Dots within the bar charts represent datapoints generated for alveolar BC from each patient. Data are expressed as mean ± SEM (Mann–Whitney test, * indicates p < 0.05)