Fig. 4

The effect of anti-CD147 in OVA-induced recruitment of circulating fibroblasts in asthmatic mice

Asthmatic mice were induced by OVA, and treated with anti-CD147 or Isotype antibody. Mice treated only with PBS were used as the control. (A) Representative flow cytometry images revealed the percentage of CD45⁺ and COL-I⁺ cells. (B) Quantification of CD45⁺COL-I⁺ fibrocytes in BALF of mice (n = 3). (C) Representative immunohistofluorescence images stained with antibodies to CD45 and COL-I revealed the presence of CD45⁺COL-I⁺ fibrocytes in lung tissue sections. COL-I appears green, CD45 appears red, and nuclei appear blue. Magnification ×200, scale bar = 100 μm. (D) Quantification of the number of CD45⁺COL-I⁺ fibrocytes in lung tissue sections (n = 3). (E) Representative western blotting images of COL-I, Fibronectin and TGF-β1 and quantification of their protein expression normalized to β-Tubulin (n = 3). Data were presented as mean ± SD, and analyzed by one-way ANOVA with Bonferroni correction. *P < 0.05, vs. control; ^#P < 0.05, vs. OVA